2002 Fiscal Year Final Research Report Summary
ANALYSIS OF THE SIGNAL TRANSDUCTION OF CELL MOTILITY INDUCED BY EGF STIMULATION IN HUMAN ORAL CANCER CELLS
| Project/Area Number |
13672115
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | GIFU UNIVERSITY |
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Principal Investigator |
SHIBATA Tohiyuki GIFU UNIV., SCHOOL OF MED., PROFESSOR, 医学部, 教授 (50226172)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Yukihiro GIFU UNIV., SCHOOL OF MED., STAFF, 医学部, 助手 (30293567)
TOIDA Makoto GIFU UNIV., SCHOOL OF MED., ASSOCIATE PROFESSOR, 医学部, 助教授 (90313890)
NAGAYASU Hiroki HEALTH SCL. UNIV. OF HOKKAIDO, SCHOOL OF DENT., ASSOCIATE PROFESSOR, 歯学部, 助教授 (90265075)
YAMASHITA Tomomi GIFU UNIV., SCHOOL OF MED., STAFF, 医学部附属病院, 助手 (80345793)
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| Project Period (FY) |
2001 – 2002
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| Keywords | HUMAN / SCC / ORAL CANCER / EGF / INVASION / MATASTASIS |
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| Research Abstract |
In order to clear the effects of Epidermal Growth Factor (EGF) on the invasion abilities of human oral squamous cell carcinoma, five cell lines were studied. EGF enhanced the random motility of all cell lines in a dose-dependent fashion and exposure to EGF let to an increased production of urokinase type plasminogen activator and matrix metalloproteniase-9 by the same cells. These results strongly suggest the EGF may promote the invasion and metastasis of human oral squamous cell carcinomas. In this project, to reveal the signal pathway, we established s-1 clone cells, which was most sensitive clone against the EGF stimulation and also we established i-1 clone cells, which was most insensitive clone. Further studies were done using s-1 clone cells. When EGF bind to the EGF receptor, tyrosine phosphorelation is firstly occurred and subsequently PKC is activated through the activation of PLCr arising from erbB homodimer and/or activation of PI3-kinase arising from erbB3 heterodimer. Furthermore, erbB homodimer activates the nPKCd through the activation of PLC and also erbB/erbB-3 heterodimer activates aPKCe through the activation of PI3K. These results suggest that inhibition of aPKCe or nPKCd can reduce the invasion and metastasis of human oral cancer cells and that the useful model in the investigations of molecular target medicines is possibly established.
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[Publications] Hamada, J., Nakata, D,.Nakae, D., Kobayashi, Y., Akai, H., Konishi, Y., Okada, F., Shibata, T., et al.-2: "Increased oxidative DNA damage, 8-hydroxydeoxyguanosine, in rat ER-1 mammary tumor cells by continuous EGF-stimulation"J. Natl. Cancer Inst. 93. 214-219 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] T. Kobayashi, F. Okuda, N. Fuji, N. Tomita, S. Ito, H, Tazawa, T. Aoyama, Sung Ki Choi, T. Shibata, et al.-3: "Thymosin-β 4 Regulates Motility and Metastasis of Malignant Monse Fibrosarcoma Cells"American J. of Pathology. 160, (3). 869-875 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hamada, J., Nakata, D., Nakae, D., Kobayashi, Y., Akai, H., Konishi, Y., Okada, F., Shibata, T., et al.-2: "Increased oxidative DNA damage, 8-hydroxydeoxygnanosine, in rat ER-1 mammary tumor cells by continuous EGF-stimulation"J. Nati. Cancer Inst. 93. 214-9 (2001)
Description
「研究成果報告書概要(欧文)」より
