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2002 Fiscal Year Final Research Report Summary

Fundamental examination of repeat structure of the Y chromosome STR locus

Research Project

Project/Area Number 13672177
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 矯正・小児・社会系歯学
Research InstitutionKanagawa Dental College

Principal Investigator

YAMADA Yoshihiro  Kanagawa Dental College, Assistant Professor, 歯学部, 助教授 (60277902)

Project Period (FY) 2001 – 2002
Keywordsforensic medicine / personal identification / Paternity / Y-STR / repeat structure / direct sequencing
Research Abstract

Recently described Y short tandem repeat (Y-STR) polymorphisms can be analyzed as informative haplotypes, which are useful in the forensic field. In order to include these systems in our forensic routine, sequence analysis was conducted about the 7 kinds of range (the locus) of DYS19, DYS389 I, DYS389 II, DYS390, DYS391, DYS392 and DYS393.
DYS391 showed the repetition of unique, simple unit. DYS389 I, DYS389 II, DYS390 and DYS393 showed the repetition that irregularly, 2 kinds of different units, were mixed each other. Furthermore, there was the insertion of a special arrangement in the font or middle of the unit which DYS19 and DYS392 were decide.
We have investigated a case of disputed paternity where the alleged father was dead, using data from the surviving children for eight Y-STR loci (DYS19, DYS389 I, DYS389 II, DYS390, DYS391, DYS392, DYS393 and DYS385: two loci). Various alleles for each system (except DYS385) were sequenced. In the eight Y-STRs both the two legitimate children and a further additional child of the deceased putative father carried the same allele. The present study demonstrates the structure of Y-STRs and the power of Y-STR typing as a tool for solving this kind of case.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Yoshihiro Yamada: "Possibility of paternity testing using RFLP analysis on a very small amount of material"J Forens Odonto-Stomatol. 19. 1-4 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshihiro Yamada: "Expression of paraoxonase isoform did not confer protection from acute sarin poisoning in the Tokyo subway terrorist attack"Int J Legal Med. 115. 82-84 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshihiro Yamada: "Dental information (postmortem dental roentgenoloey, the typing of DNA from a tooth) plays a conclusive role in dental identification"Proceedings of the 16^<th> IAFS. 153-156 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 武市早苗: "DNA多型9"東洋書店. 362 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 武市早苗: "DNA多型10"東洋書店. 290 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshihiro Yamada.: "Possibility of paternity testing using RFLP analysis on a very small amount of material"J Forens Odonto-stomatol. 19. 1-4 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshihiro Yamada.: "Expression of paraoxonase isoform did not confer protection from acute sarin poisoning in the Tokyo subway terrorist attack"Int J Legal Med.. 115. 82-84 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroshi Ohira.: "Identification applying mitochondrial DNA analysis from teeth of a drowned body that existed in water for along term"DNA Polymorphism. 9. 132-134 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroyoshi Koyama.: "DNA analysis of dismembered and aged corpse discovered in a port"DNA Polymorphism. 9. 339-342 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroshi Ohira.: "DNA extract from root canal filled tooth, and investigation of possibility of application to individual iden tification"DNA Polymorphism. 10. 262-263 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshihiro Yamada.: "Dental information (postmortem dental roentgenology, the typing of DNA from a tooth) plays a conclusive role in dental identification"Proceedings of the 16^<th> Iafs. 153-156 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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