2003 Fiscal Year Final Research Report Summary
Development of a Method for Testing the Salivary Ability against the Formation of Calcium Phosphate Precipitates
| Project/Area Number |
13672182
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | Fukuoka College of Health Sciences |
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Principal Investigator |
HIDAKA Saburo Fukuoka College of Health Sciences, Dept.of Dental Hygiene, Prof., 歯科衛生学科, 教授 (00084252)
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| Co-Investigator(Kenkyū-buntansha) |
KATSUKI Mariko Fukuoka Coll.Health Sci., Dental Hygiene, Assistant Prof., 歯科衛生学科, 助手 (70300465)
MATSUO Tadayuki Fukuoka Coll.Health Sci., Dental Hygiene, Lecturer, 歯科衛生学科, 講師 (00258584)
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| Project Period (FY) |
2001 – 2003
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| Keywords | Calcium phosphate precipitation / Bromothymol blue / pH drop method / Human whole saliva |
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| Research Abstract |
Comparing the pH drop method using a pH meter for human salivary ability against the formation of calcium phosphate precipitates, we studied a convenient method using a pH indicator, bromothymol blue (BTB). The addition of 250 μM NaF widened the range of color change of BTB. The addition of 2.5, 5.9 and 7.5 % (v/v) of whole saliva in the reaction mixture containing 250 μM NaF showed yellow, blue green, and blue, respectively, after incubation at 37℃ for 40 min. This indicated that the grade of inhibition with whole saliva of the formation of calcium phosphate precipitates was semi-quantitatively assessed by the BTB colorization. In practice, the evaluation of inhibitory ability against the formation of calcium phosphate precipitates using 10 individuals' whole saliva was carried out. The correlation coefficient(r) between the induction time (from pH drop method) and the pH value (from BTB method using the color standard) was higher (0.79〜0.86). The result strongly suggests that the inhibitory capability of human whole saliva on oral calcification (re-mineralization and calculus formation) may be easily evaluated by the method using BTB.
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