2002 Fiscal Year Final Research Report Summary
Study on the Pathogenesis of Periodontal Disease assessed from the Standpoint of Aging, especially from the Telomere Length
| Project/Area Number |
13672195
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Meikai University |
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Principal Investigator |
SHIN Kitetu Meikai University, School of Dentistry, Professor, 歯学部, 教授 (40187555)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Keiso Meikai University, School of Dentistry, Lecturer, 歯学部, 講師 (70243475)
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| Project Period (FY) |
2001 – 2002
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| Keywords | telomere length / periodontitis / aging / risk factor / early-onset periodontitis |
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| Research Abstract |
Various host and genetic risk factors may help in explaining the progression of early-onset periodontitis (EOP). We have hypothesized that premature aging could be implicated in the progression of EOP. The aim of this study was therefore to investigate the telomere length of white blood cells (WBC) and/or the change ratio of telomere length in human gingival fibroblasts (HGF) from EOP patients. WBC was obtained from 21 EOP patients and 50 periodontally healthy age-matched subjects (HS). HGF obtained from 6 EOP patients and 7 HS were cultured until those cells became senescent. Genomic DNA from WBC and HGF were isolated and analyzed for telomere length by Southern blot analysis. EOP patients showed no significant shorter telomere length compared with HS. The telomere length from WBC negatively correlated with donor age and no significant difference was found between the 2 groups. Wide inter-individual variation of telomere length from WBC was observed. No significant difference was found in the change of ratio of telomere length of HGF between the 2 groups. Telomere length negatively correlated with the percentages of β-galactosidase positive cells from HGF. These results suggest that this study does not support the hypthesis that premature aging is implicated in the progression of EOP both at general and local level, however, there was an EOP patient who extremely showed short telomere length from both WBC and HGF. Further studies are required to assess the association of cellular function of a subgroup of EOP patients who showed short telomere length.
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