2001 Fiscal Year Annual Research Report

一般配列での3本鎖DNA形成のための新しい認識分子の設計

Research Project

Project/Area Number	13672218
Research Institution	Kyushu University
Principal Investigator	佐々木茂貴九州大学, 薬学研究院, 助教授 (10170672)
Keywords	3本鎖DNA / アンチジーン法 / 2本鎖DNA認識
Research Abstract	平成13年度には非天然型3本鎖形成を目指して設計した認識分子(WNA)をD-riboseから約10ステップで合成した。認識構造にguanosineを組み込んだ4種の異性体を分離した(WNA-7,9,a, b-G)。引き続きWNA-7,9b-Gの2種をホモプリンTFO鎖に組み込み3本鎖形成能を調べた。その結果、WNA-7b-GがT-A塩基対を選択的に安定化することを見出した(TL,42,6915(2001),特許出願)。さらに少量の異性体WNA-7,9a-GをTFO鎖に組み込み3本鎖形成能を調べたところ、WNA-9a-Gはさらに安定な3本鎖をTA塩基対と生成し、WNA-7a-GはGCおよびCGを安定化することが分かった。本年度の研究により従来達成できていなかったTA塩基対の認識を達成できたばかりでなく、CG塩基対に親和性の高い認識構造を明らかにすることができた。平成14年度中には一般配列の認識を達成する予定である。

Research Products
(7 results)

All Other

All Publications (7 results)

[Publications] Sasaki, Shigeki: "Active Oligonucleotides Incorporating Alkylating Agent as Potential Sequence-and Base Selective Modifier of Gene Expression,"Eur. J. Phar. Sci.. 13(1). 43-51 (2001)
[Publications] Sasaki, Shigeki, Shibata, T., Torigoe, H., Shibata, Y., Minoru, M.: "Novel Class of DNA Binding Motifs Based on Bistetrahydrofuran and Bisfuran Skeleton with Long Alkyl Chains"Nucleoside, Nucleotides and Nucleic Acids,. 20(4-7). 551-558 (2001)
[Publications] Nagatsugi, F., Kawasaki, T., Tokuda, N., Naeda, M., Sasaki, S.: "Site-Directed Alkylation to Cytidine within Duplex by the Oligonucleotides Containing Functional Nucleobases, Bistetrahydrofuran and Bisfuran Skeleton with Long Alkyl Chains,"Nucleoside, Nucleotides and Nucleic Acids,. 20(4-7). 519-521 (2001)
[Publications] Sasaki, S., Yamauchi, H., Nagatsugi, F., Takahashi, R., Taniguchi, Y., Minoru M.: "W-Shape Nucleic Acid (WNA) for Selective Formation of Non-Natural Anti-Parallel Triplex Including a TA Interrupting Site"Tetrahedron Letters. 42(39). 6915-6918 (2001)
[Publications] Sasaki, S., Ehara, T., Alam, Md Rowshon., et al.: "Solid-Phase Synthesis of a Library Constructed of Aromatic Phosphate, Long Alkyl Chains and Tryptophane Components, and Identification of Potent Dipeptide Telomerase Inhibitors"Bioorg & Med. Chem Lett. 11(19). 2581-2584 (2001)
[Publications] Nagatsugi, Fumi., Tokuda, Natsuko., Maeda, Minoru., Sasaki, Shigeki: "A New Reactive Nucleoside Analog for Highly Reactive and Selective Cross-linking Reaction to Cytidine Under Neutral Conditions"Bioorg & Med. Chem Lett. 11(19). 2577-2579 (2001)
[Publications] 佐々木茂貴, 永次史: "多比良和誠編集「遺伝子機能破壊実験法」、第1章-8"羊土社. 83-89 (2001)

2001 Fiscal Year Annual Research Report

一般配列での3本鎖DNA形成のための新しい認識分子の設計

Principal Investigator

佐々木 茂貴 九州大学, 薬学研究院, 助教授 (10170672)

Research Products

[Publications] Sasaki, Shigeki: "Active Oligonucleotides Incorporating Alkylating Agent as Potential Sequence-and Base Selective Modifier of Gene Expression,"Eur. J. Phar. Sci.. 13(1). 43-51 (2001)

[Publications] Sasaki, Shigeki, Shibata, T., Torigoe, H., Shibata, Y., Minoru, M.: "Novel Class of DNA Binding Motifs Based on Bistetrahydrofuran and Bisfuran Skeleton with Long Alkyl Chains"Nucleoside, Nucleotides and Nucleic Acids,. 20(4-7). 551-558 (2001)

[Publications] Sasaki, S., Yamauchi, H., Nagatsugi, F., Takahashi, R., Taniguchi, Y., Minoru M.: "W-Shape Nucleic Acid (WNA) for Selective Formation of Non-Natural Anti-Parallel Triplex Including a TA Interrupting Site"Tetrahedron Letters. 42(39). 6915-6918 (2001)

[Publications] Sasaki, S., Ehara, T., Alam, Md Rowshon., et al.: "Solid-Phase Synthesis of a Library Constructed of Aromatic Phosphate, Long Alkyl Chains and Tryptophane Components, and Identification of Potent Dipeptide Telomerase Inhibitors"Bioorg & Med. Chem Lett. 11(19). 2581-2584 (2001)

[Publications] Nagatsugi, Fumi., Tokuda, Natsuko., Maeda, Minoru., Sasaki, Shigeki: "A New Reactive Nucleoside Analog for Highly Reactive and Selective Cross-linking Reaction to Cytidine Under Neutral Conditions"Bioorg & Med. Chem Lett. 11(19). 2577-2579 (2001)

[Publications] 佐々木茂貴, 永次 史: "多比良和誠編集「遺伝子機能破壊実験法」、第1章-8"羊土社. 83-89 (2001)

佐々木茂貴九州大学, 薬学研究院, 助教授 (10170672)

[Publications] 佐々木茂貴, 永次史: "多比良和誠編集「遺伝子機能破壊実験法」、第1章-8"羊土社. 83-89 (2001)