2002 Fiscal Year Final Research Report Summary
Development of simultaneous analysis apparatus for endcrine disrupters (Eds) by capillary electrophoresis
| Project/Area Number |
13672263
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | MEIJI PHARMACEUTICAL UNIVERSITY |
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Principal Investigator |
KATAYAMA Masatoki MEIJI PHARMACEUTICAL UNIVERSITY FACULTY OF PHARMACY ASSISTANT PROFESSOR, 薬学部, 講師 (20301575)
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| Co-Investigator(Kenkyū-buntansha) |
KANEKO Satoru TOKYO MEDICAL AND DENTAL UNIVERSITY FACULTY OF Obs. And Gyn. ASSISTANT PROFESSOR, 歯学部, 講師 (40214457)
ISHIKAWA Hiromichi TOKYO MEDICAL AND DENTAL UNIVERSITY FACULTY OF Urology ASSOCIATE PROFESSOR, 歯学部, 助教授 (60112679)
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| Project Period (FY) |
2001 – 2002
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| Keywords | endocrine disruptor / capillary electrophoresis / obstorical and gynecology / bisphenol A / nonylphenol / in human sperm / urology / sperm quality |
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| Research Abstract |
2001
We have aimed to develop the simultaneous determination method for EDs (category III) by CE. Bisphenol A, octylphenol and nonylphenol were selected and detected by UV detection CE in 2001. We have studied and optimized CE condition (MEKC, Zone). As the result, each phenol could be determined at 10 pg/mL level. Further, we have determined Bisphenol A and nonylphenol in mother and infant blood to standardize the extraction condition from biological samples (e.g., human serum, urine and sperm). By established extraction conditions, we have determined EDs in biological samples. And confirmed the CE method advantages (sensitivity, easy operation and running cost) in compare with HPLC and GC-MS.
2002
The pureness of sperm quality have reported in urological, obstetrical and gynecological section. We have determined bisphenol A and nonylphenol in human sperm from 64 patients in urological section after informed consent. These data were studied the relationships among shape and number data of sperm in view of urology, obstetric and gynecology. We have also started to develop the database in epidemiological investigation.
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