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2002 Fiscal Year Final Research Report Summary

Characteristics of flurothyl-induced seizures and the evaluation of the model of development of epilepsy

Research Project

Project/Area Number 13672388
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用薬理学・医療系薬学
Research InstitutionEhime University (2002)
Okayama University (2001)

Principal Investigator

ARAKI Hiroaki  Ehime University Hospital, Professor, 医学部附属病院, 教授 (50294450)

Co-Investigator(Kenkyū-buntansha) GOMITA Yutaka  Okayama University Hospital, Professor, 医学部附属病院, 教授 (00088709)
Project Period (FY) 2001 – 2002
Keywordsflurothyl / kindling / MK-801 / Mongolian gerbils / Electroencephalogram / antiepileptic drugs
Research Abstract

1) In the flurothyl kindling model, eight flurothyl-induced generalized clonic seizures, dubbed forebrain seizures, and a subsequent 28-days stimulation-free interval changed the seizure phenotype of mice from purely forebrain seizure to seizures of a more complex phenotype. The generalized-onset, forebrain seizure progresses rapidly to a seizure with tonic manifestations, dubbed brain-stem seizures, when re-challenged with flurothyl.
2) When valproate, zonisamide and phenytoin were administered to mice daily for 8 d prior to the flurothyl trial changes in the seizure phenotype from clonic to tonic seizure were significantly inhibited by valproate and zonisamide, but not by phenytoin.
3) In the acute effect of MK-801 in naive and flurothyl-kindled mice, there were no changes on the latencies of clonic seizures. However, MK-801 dose-dependently inhibited both latencies and the incidence of tonic seizures in mice and suppressed the grade of seizure severity in flurothyl-kindled mice. In addition, repeated administration of MK-801 did not have any effect on the latencies of clonic seizure. However, seizure severity was significantly exacerbated when mice were re-exposed to flurothyl without MK-801 1 week after the last administration.
4) Mongolian gerbils demonstrated tonic extension immediately after within 1 min after the appearance of clonic convulsion. Very high amplitude spike waves appeared in the cortex, hippocampus and amygdala concurrent with the appearance of clonic convulsion.
5) The epilepsy pattern was not changed in 6-OHDA- and DSP-4-administered mice. This finding is completely different with electrical kindling model.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] H.Araki, Y.Kobayashi, Y.Hashimoto, K.Futagami, H.Kawasaki, Y.Gomita: "Characteristics of flurothyl-induced seizures and the effect of anti-epileptic drugs on flurothyl-induced seizures in Mongolian gerbils"Pharmacology, Biochemistry and Behavior. 74. 141-147 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Hashimoto, H.Araki, Y.Gomita: "Cessation of repeated administration of MK-801 changes the anticonvulsant effect against flurothyl-induced seizure in mice"Pharmacology, Biochemistry and Behavior. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Hashimoto, H.Araki, K.Futagami, H.Kawasaki, Y.Gomita: "Effect of valproate, phenytoin and zonisamide on clonic and tonic seizure induced by acute and repeated inhalation of fluroyjul in mice"Physiology and Behavior. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroaki Araki, Yuri Kobayashi, Yasuhiko Hashimoto, Koujiro Futagami, Hiromu Kawasaki and Yutaka Gomita: "Characteristics of flurothyl-induced seizures and the effect of anti-epileptic drugs on flurothyl-induced seizures in Mongolian gerbils"Pharmacology, Biochemistry and Behavior. 74. 141-147 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuhiko Hashimoto, Hiroaki Araki and Yutaka Gomita: "Cessation of repaeted administration of MK-801 changes the anticonvulsant effect against flurothyl-induced seizure in mice"Pharmacology, Biochemistry and Behavior. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasuhiko Hashimoto, Hiroaki Araki, Koujiro Futagami, Hiromu Kawasaki and Yutaka Gomita: "Effect of valproate, phenytoin and zonisamide on clonic and tonic seizure induced by acute and repeated inhalation of fluroyjul in mice"Physiology and Behavior. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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