2003 Fiscal Year Final Research Report Summary
Non-thermal methods to disinfect enterohemorhagic Escherichia coli contamination in salad vegetables
| Project/Area Number |
13680154
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
食生活
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| Research Institution | Osaka City University |
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Principal Investigator |
NISHIKAWA Yoshikazu Osaka City University, Graduate School of Human Life Science, Associate Professor, 大学院・生活科学研究科, 助教授 (60183539)
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| Project Period (FY) |
2001 – 2003
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| Keywords | EHEC / O157 / decontamination / non-thermal / Food / vegetables / pulsed electric fields / heated shell powder |
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| Research Abstract |
A strain of enterohemorrhagic Escherichia coil O157 was transformed by the plasmid which contained the green fluorescence protein gene. Artificially contaminated lettuce was produced by soaking the leaves in the suspension of the bacterium. Neglecting preexisting bacterial flora the number of O157 were accurately counted for their fluorescence under irradiation of UV. Throughout this research various kinds of chemical reagents and treatment-conditions were examined for disinfecting effects. Heated shell-powder solution showed strong bactericidal activity on lettuce rather than hypochlorite. A part of the inoculum seemed to have invaded to lettuce organization inside where the drug is difficult to reach and perfectly kills it. The O157 organisms were killed in 8-24 hour, when the lettuce was preserved at the low temperature after soaking in the shell-powder solution. Biopreservation is becoming attractive method for food safety. Although bacteria showing inhibitory effects against O157 were isolated from the human fecal specimens, it was not found among normal bacterial flora of foods throughout the experiments. It was suggested through some preliminary experiments that pulsed power electric discharge could possibly reduce the level of O157 contamination on lettuce.
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[Publications] Zhou, Z., Nishikawa, Y., Zhu, P., Hong, S., Hase, A., Cheasty, T., Smith, H.R., Zheng, M., Haruki, K.: "Isolation and characterization of Shiga toxin-producing Escherichia coli O157:H7 from beef, pork and cattle fecal samples in Changchun, China."J.Vet.Med.Sci.. 64. 1041-1044 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Nishikawa, Y., Zhou, Z., Hase, A., Ogasawara, J., Kitase, T., Abe, N., Nakamura, H., Wada, T., Ishli, E., Haruki, K., the Surveillance Team.: "Diarrheagenic Escherichia coli isolated from stools of sporadic cases of diarrheal illness in Osaka City, Japan between 1997 and 2000 : prevalence of enteroaggregative E.coil heat-stable enterotoxin 1 gene-possessing E.coli."Jpn.J.Infect.Dis.. 55. 183-190 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Nishikawa, Y., Hase, A., Ogasawara, J., Cheasty, T., Willshaw, G.A., Smith, H.R., Tatsumi, Y., Yasukawa, A.: "Phage typing and DNA-based comparison of strains of enterohemorrhagic Escherlchia coli O157 from apparently sporadic infections in Osaka City, Japan 1996."Jpn.J.Infect.Dis.. 54. 140-143 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Zhou, Z., Nishikawa, Y., Zhu, P., Hong, S., Hase, A., Cheasty, T., Smith, H.R., Zheng, M., Haruki, K.: "Isolation and characterization of Shiga toxin-producing Escherichia coli O157: H7 from beef, pork and cattle fecal samples in Changchun, China"J.Vet.Med.Sci.. 64. 1041-1044 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nishikawa, Y., Zhou, Z., Hase, A., Ogasawara, J., Kitase, T., Abe, N., Nakamura, H., Wada, T., Ishii, E., Haruki, K., the Surveillance Team: "Diarrheagenic Escherichia coli isolated from stools of sporadic cases of diarrheal illness in Osaka City, Japan between 1997 and 2000: prevalence of enteroaggregative E.coli heat-stable enterotoxin 1 gene-possessing E.coli"Jpn.J.Infect.Dis.. 55. 183-190 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nishikawa, Y., Hase, A., Ogasawara, J., Cheasty, T., Willshaw, G.A., Smith, H.R., Tasumi, Y., Yasukawa, A.: "Phage typing and DNA-based comparison of strains of enterohemorrhagic Escherichia coli O157 from apparently sporadic infections in Osaka City, Japan, 1996"Jpn.J.Infect.Dis.. 54. 140-143 (2001)
Description
「研究成果報告書概要(欧文)」より
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