2002 Fiscal Year Final Research Report Summary
The Development of the Novel Reactive Nucleobases to the Duplex DNA
| Project/Area Number |
13680673
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
NAGATSUGI Fumi Faculty of Pharmaceutical Sciences, Assistant Prof., 薬学研究院, 助手 (90208025)
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| Project Period (FY) |
2001 – 2002
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| Keywords | 2-amino-6-vinilpurine / antisense / reactive nucleic acids / genome targeted chemistry / point mutation by chemical method |
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| Research Abstract |
The selective reaction to the gene would have the potential for site-directed mutation. In 2001, we have demonstrated that TFO bearing 2-amino-6-vinypurine derivative achieves triplex mediated cross-linking with high selectivity toward the cytosine of the G-C or the adenosine of the T-A target site. In 2002, we have investigated the use of this reactive TFO to target mutations to a target site in a shuttle vector plasmid, which replicates in mammalian cells. TFOs bearing of this reactive molecular produced adducts only at the complementary position of this reactive molecular and thereby introduced mutations at that site during replication/repair of the plasmid in mammalian cells. In the addition, we attempted the improvement of the reactivity by some modification of this molecule. But this experiment was not successful, because the oligonucleotides bearing modified molecule were not able to form the stable triple helix. It was clear that the triple helix formation was effected by a small change of structure. Now we investigate to develop of the new molecular by using the post modification method and we expect that we can get a new molecule with effective reactivity to gene.
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[Publications] Sasaki, S., Yamauchi, H., Nagatsugi.F., Takahashi, R., Taniguchi, Y., Maeda, M.: "W-Shape Nucleic Acid (WNA) for Selective Formation of Non-Natural Anti-Parallel Triplex Including a TA Interrupting Site"Tetrahedron Letters. 42(39). 6915-6918 (2001)
Description
「研究成果報告書概要(和文)」より
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