2002 Fiscal Year Final Research Report Summary
Studies on the physiological function of the lux operon in luminous bacteria and the induction mechanism of the operon.
| Project/Area Number |
13680721
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | OSAKA CITY UNIVERSITY |
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Principal Investigator |
KASAI Sabu OSAKA CITY UNIVERSITY, FACULTY OF ENGINEERING, ASSOCIATE PROF., 大学院・工学研究科, 助教授 (90047340)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Luminous bacteria / lux operon / biosynthesis of riboflavin / Photobacterium phosphoreum / Vibrio fischeri / rib operon / SUGDAT / Pulse-field gel electrophoresis |
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| Research Abstract |
Stimulated biosynthesis of flavins in Photobacterium phosphoreum and the presence of complete rib operons in two species of luminous bacteria: It was confirmed that P. phosphoreum produces large amount of flavins, when it emits light strongly. From these results, we deduced that the genes for riboflavin synthesis are organized in an operon in this strain. A partial ribD sequence was amplified and sequenced using a PCR-based method, and subsequently the genomic DNA was sequenced in both directions from this partial sequence using SUGDAT and we found a complete rib operon in this species. Furthermore, we sequenced the rib operon in Vibrio flscheri and confirmed that a complete operon is present also in this species.
Distribution of regulatory genes for the lux operon in V. fischeri and P. phosphoreum: Regulatory genes for the lux operon, lux M, N, O, P, Q, R2, and S, and pfs, were found systematically in V. harveyi. To confirm that these genes are present not only in V. harveyi but also i
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n other species of luminous bacteria, we tried to find these genes in V. fischeri and P. phosphoreum. In V. fischeri, all these genes were found but in P. phosphoreum, luxP and Q were not found and it was concluded that the 4, 5-dihydroxy 2, 3-pentanedione mediated regulatory system is not present in this species. Because luxM, N and R2 were present in addition to luxI and R, in V. fischeri, it is questionable that luxI and R are regulatory genes for the lux operon.
Location of the lux operon in the genome of luminous bacteria: Among luminous bacteria, genes present in both flanking regions of the lux operon, are not the same. This evidence indicates that the operons are not fixed in the specific position in the genomes. To find general law to locate the operon in the genome, genes in both flanking regions were inspected. In Vibrionaceae, three genomic sequences have already appeared in the database and almost all orthologes of the genes neighboring the lux operons belong to chromosome II. From these results, we concluded that the lux operons in Vibrionaceae were present in the chromosome II. Less
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Research Products
(2 results)
