2002 Fiscal Year Final Research Report Summary
Single-molecule detection of groove-tracking of DNA by RNA polymerase during sliding
| Project/Area Number |
13680753
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | RIKEN (2002)
National Institute of Genetics (2001) |
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Principal Investigator |
SOGAWA Kumiko RIKEN, Cell Signaling Team, Research Scientist, 免疫制御モジュール研究チーム, 研究員 (20291073)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMAMOTO Nobuo National Institute of Genetics, Structural Biology Center, Professor, 構造遺伝学研究センター, 教授 (20127658)
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| Project Period (FY) |
2001 – 2002
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| Keywords | DNA binding protein / non-specific binding protein / sliding / RNA polymerase / optical tweezers / single molecule manipulation / fluorescence microscopy |
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| Research Abstract |
One-dimensional diffusion of proteins along DNA can facilitate their recognition of the sites at which they form functional complexes. One possible facilitating mechanism is tracking a DNA groove during sliding, which consists of consecutive transfers into adjacent DNA sites along a DNA groove without complete dissociation. By visualizing the relative rotational movement between DNA and single molecules of E.coli RNA polymerase holoenzyme, we here show that RNA polymerase can track a DNA groove. Our result also confirms the existence of sliding, which is still sometimes debated despite much supporting evidence based on kinetic and single molecule studies. Not only facilitating the recognition, this groove tracking help to distinguish double-stranded B-helix DNA from other macromolecules in the cell.
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