ANALYSIS OF CHROMOSOMESEGREGATION MECHANISM BY HUMAN CH-TOG RELATED GENES IN YEAST

2003 Fiscal Year Final Research Report Summary

• Project/Area Number: 13680779
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: KYOTO UNIVERSITY
• Principal Investigator: NAKASEKO Yukinobu KYOTO UNIVERSITY, GRADUATE SCHOOL OF BIOSTUDIES, ASSOCIATE PROFESSOR, 生命科学研究科, 助教授 (30231468)
• Project Period (FY): 2001 – 2003
• Keywords: KINETOCHORE / CHROMOSOME / CANCER CELL / CHROMOSOME SEGREGATION / YEAST

Research Abstract

Fission yeast S. pombe dis1 mutants are defective in sister chromatids separation. Dis1 belongs to an evolutionarily conserved family of microtubule binding proteins, including human ch-TOG, frog XMAP215, fly minispindles and budding yeast Stu2p. We found Dis1-GFP signals are associated with cytoplasmic microtubules, spindle microtubules and spindle pole bodies. Surprisingly, as cells progress from metaphase to anaphase during mitosis, Dis1-GFP signals mimic those of kinetochore proteins such as Mis6, Mis12. Dis1 is the first fission yeast protein that associates with both microtubules and the centromeric DNA of the kinetochore. Dis1 may play a dual role by becoming a part of the kinetochores in an M-phase specific manner, possibly generating connections between kinetochores and microtubules.
We have also shown that protein highly related to Dis1, called Mtc1/Alp14, behaves in a similar but not identical manner to Dis1. Their behavior suggests that these two related molecules have significant differences in interaction with microtubules.

Research Products

• [Publications] Nakaseko, Y., Goshima, G., Morishita, J., Yanagida, M.: "M phase-specific kinetochore proteins in fission yeast : microtubule-associating Dis1 and Mtc1 display rapid separation and segregation during anaphase."Current Biology. 11. 537-549 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakamura, T., Nagao, K., Nakaseko, Y., Yanagida, M.: "Cut1/separase C-terminus affects spindle pole body positioning in interphase of fission yeast pointed nuclear formation."Gents to Cells. 7・11. 1113-1124 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Fukumoto, Y., Hiyama, H., Yokoi, M., Nakaseko, Y., Yanagida, M., Hanaoka, F.: "Two budding yeast RAD4 homologs in fission yeast play different roles in the repair of UV-induced DNA damage."DNA repair. 1・10. 833-845 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakaseko, Y., Goshima, G., Morishita, J., Yanagida, M.: "M phase-specific kinetochore proteins in fission yeast: microtubule-associating Dis1 and Mtcl display rapid separation and segregation during anaphase."Current Biology. Vol.11. 537-549 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakamura, T., Nagao, K., Nakaseko, Y., Yanagida, M.: "Cut1/separase C-terminus affects spindle pole body positioning in interphase of fission yeast pointed nuclear formation."Genes to Cells. Vol.7 No.11. 1113-1124 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Fukumoto, Y., Hiyama, H., Yokoi, M., Nakaseko Y., Yanagida, M., Hanaoka, F.: "Two budding yeast RAD4 homologs in fission yeast play different. roles in the repair of UV-induced DNA damage."DNA repair. Vol.1 No.10. 833-845 (2002)
  • Description: 「研究成果報告書概要(欧文)」より