2002 Fiscal Year Final Research Report Summary
Analysis of chromosomal instability using cohesin deficient chicken B cell line
| Project/Area Number |
13680782
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SONODA Eiichiro Kyoto University, Faculty of Medicine, Dept. of Radiation Genetics, associate professor, 医学研究科, 助教授 (50281093)
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| Project Period (FY) |
2001 – 2002
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| Keywords | Sister Chromatid Cohesion / Rad21 / Kinetochore function / Chromosomal instability / DT40 / Cohesin / IN CENP |
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| Research Abstract |
Proteolytic cleavage of the cohesin subunit Rad21/Scc1 is a consistent feature of anaphase onset, although temporal differences exist between eukaryotes in cohesin loss from chromosome arms, as distinct from centromeres. We examined the effects of genetic deletion of Rad21/Scc1in chicken DT40 cells by using tet-repressive conditional system. We found that vertebrate Scc1 is an essential protein and is required for sister chromatid cohesion in interphase and mitosis, but not for chromosome condensation. Loss of sister chromatid cohesion also impedes the homologous recombinational repair of DNA damage. Chromosome misalignment at metaphase results in a miitotic arrest or delay in Scc1deficient cells, with aberrant disjunction at anaphase-potentially implicating *ohesion defects in aneuploidy. Surprisingly, we also found that Scc1 deficiency disrupts the centromere localization of the chromosome passenger, INCENP. These findings suggest additional roles for Scc1 at centromeres, in the control of kinetochore function per se
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[Publications] Sonoda, E., Matsusaka, T., Morrison, C., Vagnarelli, P., Hoshi, O., Ushiki, T., Nojima, K., Fukagawa, T., et al.: "Scc1/Rad21/Mcd1 is required for sister chromatid cohesion and kinetochore function in vertebrate cells"Developmental Cell. 1. 759-770 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Sonoda, E., Morrison, C., Yamashita, Y.M., Takata, M., Takeda, S.: "Reverse genetic studies of homologous DNA recombination using the chicken B-lymphocyte line, DT40"Philos Trans R Soc Lond B Biol Sci. 356. 111-117 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Sonoda,E., Matsusaka,T., Morrison,C., Vagnarelli,P., Hoshi,O., Ushiki,T., Nojima,K., Fukagawa,T.: "Scc1/Rad21/Mcd1 is required for sister chromatid cohesion and kinetochore function in vertebrate cells"Developmental Cell. 1. 759-770 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sonoda,E., Morrison,C., Yamashita,Y.M., Takata,M., Takeda,S.: "Reverse genetic studies of homologous DNA recombination using the chicken B-lymphocyte line, DT40"Philos Trans R Soc Lond B Biol Sci. 356. 111-117 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Okada,T., Sonoda,E., Yamashita,Y.M., Koyoshi,S., Tateishi,S., Yamaizumi,M., Takata,M., Ogawa,O., Takeda,S.: "Involvement of vertebrate polkappa in Rad18-independent postreplication repair of UV damage"J Biol Chem. 277. 48690-48695 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamashita,Y,M, Okada,T, Matsusaka,T, Sonoda,E, Zhao,G,Y, Araki,K, Tateishi,S, Yamaizumi,M, Takeda,S: "RAD18 and RAD54 cooperatively contribute to maintenance of tenomic stability in vertebrate cells"EMBO J. 21. 5558-5566 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Morrisoa,C, Vagnarelli,P, Sonoda,E., Takeda,S, Earnshaw,W,C: "Sister chromatid cohesion and genome stability in vertebrate cells"Biochem Soc Trans. 31. 263-265 (2003)
Description
「研究成果報告書概要(欧文)」より
