2002 Fiscal Year Final Research Report Summary
Mechanism of initiation of development via an increase of intracellular pH
| Project/Area Number |
13680801
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Ochanomizu University |
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Principal Investigator |
CHIBA Kazuyoshi Ochanomizu University, Biology, Associate Professor, 理学部, 助教授 (70222130)
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| Project Period (FY) |
2001 – 2002
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| Keywords | pH / Starfish / maturation / arrest / meiosis / MAP kinase / oocyte |
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| Research Abstract |
Reinitiation of meiosis in oocytes usually occurs as a two-step process during which release from the prophase block is followed by an arrest in metaphase of the first or second meiotic division (metaphase I [MI] or metaphase II [MII]). The mechanism of MI arrest in meiosis is poorly understood, although it is a widely observed phenomenon in invertebrates. The blockage of fully grown starfish oocytes in prophase of meiosis I is released by the hormone 1-methyladenine. It has been believed that meiosis of starfish oocytes proceeds completely without MI or MII arrest, even when fertilization does not occur. Here we show that MI arrest of starfish oocytes occurs in the ovary after germinal vesicle breakdown. This arrest is maintained both by the Mos/MEK/MAP kinase pathway and the blockage of an increase of intracellular pH in the ovary before spawning. Immediately after spawning into seawater, activation of Na+/H+ antiporters via a heterotrimeric G protein coupling to a 1-methyladenine receptor in the oocyte leads to an intracellular pH increase that can overcome the MI arrest even in the presence of active MAP kinase.
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Research Products
(4 results)
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[Publications] Iwasaki, H., Chiba, K., Uchiyama, T., Yoshikawa, F., Suzuki, F., Ikeda, M., Furuichi, T., and Mikoshiba, K.: "Molecular characterization of the starfish 1,4,5-trisphosphate receptor and its role during oocyte maturation and fertilization"J. Biol. Chem.. 277. 2763-2772 (2002)
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