2002 Fiscal Year Final Research Report Summary
Cloning and characterization of proteins whose mRNAs are localized at the postsynaptic sites.
| Project/Area Number |
13680839
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Shinshu University |
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Principal Investigator |
SUZUKI Tatsuo Shinshu University School of Medicine, Professor, 医学部, 教授 (80162965)
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| Co-Investigator(Kenkyū-buntansha) |
TIAN Qingbao Shinshu University School of Medicine, Research Associate, 医学部, 助手 (40324264)
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| Project Period (FY) |
2001 – 2002
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| Keywords | PSD / postsynaptic density / synaptic plasticity / local protein synthesis / poetsynaptic density |
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| Research Abstract |
We cloned from a rat brain cDNA library more than ten novel full-length cDNAs, whose mRNAs are localized to postsynaptic sites (we call them Dem genes). We characterized some of the Dem proteins. 1
Results obtained are as follows.
1. synUSP : a novel ubiquitin-specific protease (USP), which is enriched in the PSD fraction and localized to dendrites in the rat brain. It possesses USP enzyme activity. We also identified an interacting protein by yeast two-hybrid method.
2. synArfGEF : a novel GEF for Art, which is enriched in the PSD fraction and localized to dendrites in the rat brain. Pull-down assay showed interaction with PSD-95 and SAP97 via a C-terminal PDZ-binding motif.
3. ssLRP : a novel LDL receptor-like protein which is localized to the postsynaptic sites and localized to dendrites in the rat brain. It interacted with PSD-95 and SAP97 via a C-terminal PDZ-binding motif. Ligands are still under investigation.
4. Another Dem protein, TANC and p55L appear to be scaffold proteins for PSD. They interacted with various other PSD proteins, including PDZ-containing proteins and cytoskeletal proteins.
5. We plan to produce knockout mice for synUSP and ssLRP genes to know their physiological functions and relation to some types of disease.
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[Publications] Konno D, KOJA, Usui S, Hori K, Maruoka H, Inui M, Fujikado T, Tano Y, Suzuki T, Tohyama K, Soreu K: "The postsynaptic density and dendritic raft localization of PSD-Zip70, which contains an N-myristoylation sequence and leucine-zipper motifs"J Cell Sci. 115. 4695-4706 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Konno D, Ko JA, Usui S, Hori K, Maruoka H, Inui M, Fujikado T, Tano Y, Suzuki T, Tohyama K and Sobue K: "The postsynaptic density and dendritic raft localization of PSD-Zip70, which contains an N-myristoylation sequence and leucine-zipper motifs"J Cell Sci.. 115. 4695-4706 (2002)
Description
「研究成果報告書概要(欧文)」より
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