• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2002 Fiscal Year Final Research Report Summary

Physiological roles of plasticity of inhibitory synaptic transmission

Research Project

Project/Area Number 13680899
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 神経・脳内生理学
Research InstitutionKanazawa University

Principal Investigator

OHNO-SHOSAKU Takako  Kanazawa University, Department of Cellular Neurophysiology assistant professor, 大学院・医学系研究科, 助教授 (60179025)

Project Period (FY) 2001 – 2002
Keywordsretrograde signal / endocannabinoid / cannabinoid receptor / synaptic modulation / hippocampus / inhibitory synaptic transmission / muscarinic receptor / metabotropic glutamate receptor
Research Abstract

1. Recent studies have revealed that the activity of postsynaptic neurons can influence presynaptic functions via endocannabinoids that are released from postsynaptic neurons and act retrogradely on presynaptic terminals. In this study, we attempted to clarify physiological roles of endocannabinoid-mediated retrograde modulation of synaptic transmissions.
2. We made paired whole-cell recordings from cultured hippocampal neurons prepared from newborn rats, and recorded evoked excitatory and inhibitory postsynaptic currents. Our results are summarized as follows. (1) Endocannabinoids can be released from postsynaptic neurons by depolarization or by activation of group I metabotropic glutamate receptors (mGluR) (Ohno-Shosaku et al., Eur J Neurosci 15, 2002). (2) Endocannabinoid release is also induced by activation of the M_1 and M_3 subtypes of muscarinic acetylcholine receptors (in preparation), (3) Postsynaptic depolarization and activation of group I mGluRs or M_1/M_3 receptors work in a cooperative manner to release endocannabinoids (Ohno-Shosaku et al., Eur J Neurosci, in press). (4) The released endocannabinoids act retrogradely onto presynaptic cannabinoid CB1 receptors and suppress the transmitter release from presynaptic terminals. (5) The cannabinoid-sensitivity of presynaptic terminals is high in a subpopulation (about 50 %) of inhibitory synapses, low in excitatory synapses, and almost absent in the rest of inhibitory synapses (Ohno-Shosaku et al., J Neurosci 22, 2002).
3. These results suggest that endocannabinoids mediate target-specific retrograde signals, by which the postsynaptic neuronal activity influences the transmitter release from certain types of presynaptic terminals.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Takako Ohno-Shosaku: "Cooperative endocannabinoid production by neuronal depolarization and group I metabotropic glutamate receptor activation"European Journal of Neuroscience. 15. 953-961 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takako Ohno-Shosaku: "Presynaptic cannabinoid sensitivity is a major determinant of depolarization-induced retrograde suppression at hippocampal synapses"The Journal of Neuroscience. 22. 3864-3872 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masanobu Kano: "Retrograde signaling at central synapses via endogenous cannabinoids"Molelular Psychiatry. 7. 234-235 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takao Ohno-Shosaku: "Postsynaptic M1 and M3 receptors are responsible for the muscarinic enhancement of retrograde endocannabinoid signaling in the hippocampus"European Journal of Neuroscience. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takako Ohno-Shosaku: "Cooperative endocannabinoid production by neuronal depolarization and group I metabotropic glutamate receptor activation"European Journal of Neuroscience. 15. 953-961 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takako Ohno-Shosaku: "Presynaptic cannabinoid sensitivity is a major determinant of depolarization-induced retrograde suppression at hippocampal synapses"The Journal of Neuroscience. 22. 3864-3872 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masanobu Kano: "Retrograde signaling at central synapses via endogenous cannabinoids"Moleluiar Psychiatry. 7. 234-235 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takako Ohno-Shosaku: "Postsynaptic M1 and M3 receptors are responsible for the muscarinic enhancement of retrograde endocannabinoid signaling in the hippocampus"European Journal of Neuroscience. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2004-04-14  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi