2002 Fiscal Year Annual Research Report
| Project/Area Number |
13770458
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
丸野 元美 琉球大学, 医学部, 助手 (00264507)
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| Keywords | リーシュマニア症 / ONO-4007 / INF-γ / meglumine antimoniate / J774cell / BALB / cマウス |
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| Research Abstract |
NO-4007(LPS誘導体)、INF-γ、meglumine antimoniateのcombination投与による抗リーシュマニア作用について
(i)リーシュマニア感染BALB/cマウスに対して;2×10^6のLeishmania amazonensis promastigotesを背部皮膚に接種2週間後より薬剤投与し、10週間病変発現を観察したところ、meglumine antimoniate+IFN-γ連日投与群で病変の発現を完全に抑制した。meglumin antimoniate連日+ONO-4007週3回投与群はmeglumine antimoniate連日+IFN-γ週3回投与群と同程度に病変発現を著明に抑制した。また薬剤単独投与よりcombination投与の有効性が示唆された。
(ii)リーシュマニア感染J774cellに対して;Leishmania amazonensisまたはmajorを感染させたJ774cellに薬剤を添加し48時間後にその上清のTNF-αをELIZA法にて測定した。またJ774cellを電顕的に観察した。その結果、ONO-4007投与群でのみTNF-αの著明な誘導を認めた。J774cell内の原虫数は薬剤投与群では著明に低下しており、parasitophorous vacuoleは薬剤投与群全てで形成抑制がみられた。これらの結果はONO-4007の他剤とは異なる原虫排除機構を示唆しており興味深い。
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Research Products
(6 results)
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[Publications] Khan MAK, Maruno M, et al.: "Inhibition of intracellular proliferation of Leishmania parasites in vitro and suppression of skin lesion development in Balb/c mice by a novel lipid A analog (ONO-4007)"Am J Trop Med Hyg.. 67(2). 184-190 (2002)
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[Publications] Khaskhely NM, Maruno M, et al.: "Low-Dose UVB Contributes to Host Resistance against Leshmania amazonensis Infection in Mice through Induction of Gamma Interferon and Tumor Necrosis Factor Alpha Cytokines"Clin Diagn Lab Immunol. 9(3). 677-686 (2002)
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[Publications] Khaskhely NM, Maruno M, et al.: "Pre-exposure with low-dose UVA suppresses lesion development and enhances Th1 response in BALB/c mice infected with Leishmania (Leishmania) amazonensis"J Dermatol Sci. 26. 217-232 (2001)
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[Publications] Maruno M, et al.: "Anti-Leishmanial Effects of LPS Derivative and IFN-γ in Experimental Leishmaniasis : A Preliminary Study of Combination Therapy with Maglumine Antimoniate"Studies on New World Leishmaniasis and its Transmission, with Particular Reference to Ecuador, Research Report Series No.6. 139-145 (2001)
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[Publications] Khan MAK, Maruno M, et al.: "Anti-Leishmanial Effect of Glucantime^<【○!R】> against Leishmania Promastigote and Amastigote Form"Studies on New World Leishmaniasis and its Transmission, with Particular Reference to Ecuador, Research Report Series No.6. 131-138 (2001)
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[Publications] Khaskhely NM, Maruno M, et al.: "An Influence of Delayed Type Hypersensitivity Reaction and Ultraviolet Light to Experimental Leishmaniasis"Studies on New World Leishmaniasis and its Transmission, with Particular Reference to Ecuador, Research Report Series No.6. 111-119 (2001)
