2002 Fiscal Year Final Research Report Summary
Establishment of screening system of environmental compounds for estrogenicity via WISP-2-induction
| Project/Area Number |
13833002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HIDEKUNI Inadera THE UNIVERSITY OF TOKYO, The University of Tokyo, Environmental Science Center, Associate Professor (10301144)
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| Project Period (FY) |
2001 – 2002
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| Keywords | estrogens / xenoestrogens / breast cancer cells / screening / WISP-2 |
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| Research Abstract |
As many structurally diverse chemicals have been reported to function as estrogens, evaluations for estrogenicity of compounds are of widespread concern. We identified WISP-2 (Wnt-1 inducible signaling pathway protein 2) as a novel estrogen-inducible gene in the MCF-7 human breast cancer cell lines. Progesterone, dexamethasone, tri-iodothyronine, and 2,3,7,8-tetrachlorodibenzo-p-dioxin did not regulate the expression of WISP-2, indicating that its induction is highly specific for hormones that interact with the estrogen receptor. Western blot analysis detected WISP-2 protein induced by 17α-estradiol(E2), not only in the cell lysates but also in the culture supernatant of exposed cells, indicating that WISP-2 was a secreted protein. The induction of WISP-2 protein by E2 in the culture supernatant was dose-dependent with estimated EC_<50> levels between 10 and 100 pM. These results demonstrated the capacity to screen environmental compounds for estrogenicity via WISP-2 induction.
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Research Products
(12 results)
