2004 Fiscal Year Final Research Report Summary
Studies on low-temperature sensors and the mechanisms of acclimation to low-temperature
| Project/Area Number |
13854002
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物生理
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| Research Institution | National Institute to Basic Biology (2004)
Okazaki National Research Institutes (2001-2003) |
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Principal Investigator |
MURATA Norio National Institute for Basic Biology, Division of Cellular Regulation, Professor, 環境適応研究部門, 教授 (90011569)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Iwane University of Tsukuba, Graduate School of Life and Environmental Sciences, Assistant Professor, 大学院・生命環境科学研究科, 講師 (10290909)
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| Project Period (FY) |
2001 – 2004
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| Keywords | Cold sensor / Histidine kinase / DNA super-coiling / Response regulater / Cyanobacterium / Ser / Thr protein kinases |
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| Research Abstract |
(1) The cyanobacterium Synechocystis sp. PCC 6803 contains 44 genes for histidine kinases (Hiks) and 42 genes for response regulators (Rres). We individually inactivated all of these signal transducers and generated knockout libraries. By screening these libraries by cold-inducible expression of genes with genome-wide DNA microarrays we identified Hik33 as only one Hik for cold sensing and Rre 26 and Rre 31 as response regulators located downstreem of Hik33 in the cold signal transduction pathway.
(2) We identified Hik33, which we previously identified as a cold sensor, as a component involved in the signal pathways of hyperosmotic, cold, strong-light and oxidative stress. These findings suggest that Hik33 itself is not specific to the individual stress and that the signal transduction pathways which include Hiks and Rres are more complicated than the simple two-component systems which have been established previously. It is very likely that Hik33 cooperates with other factors that give the specificity to individual stress. Identifications of such factors will be an important subject of further study.
(3) We demonstrated possibilities that some Ser/Thr protein kinases are involved in the cold-signal transduction pathway and that DNA supercoiling may directly sense the cold signals.
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[Journal Article] Five histidine kinases perceive osmotic stress and regulate distinct sets of genes in Synechocystis.2004
Author(s)
K.Paithoonrangsarid, M.Shoumskaya, Y.Kanesaki, S.Satoh, S.Tabata, D.A.Los, V.Zinchenko, H.Hayashi, M.Tanticharoen, I.Suzuki, N.Murata
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Journal Title
The Journal of Biological Chemistry 279
Pages: 53078-53086
Description
「研究成果報告書概要(和文)」より
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