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2004 Fiscal Year Final Research Report Summary

Analysis of coronary microcirculation and myocardial crossbridge dynamic using SPring-8 synchrotron facility

Research Project

Project/Area Number 13854030
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionOkayama University

Principal Investigator

KAJIYA Fumihiko  Okayama University, Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (70029114)

Co-Investigator(Kenkyū-buntansha) SUGA Hiroyuki  National Cardiovascular Center, Research Institute, Director General, 所長 (90014117)
YAGI Naoto  Research & Utilization Division, Japan Synchrotron Radiation Research Institute, Chief Scientist, 主席研究員 (80133940)
OHE Toru  Okayama University, Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (70263556)
SHIMIZU Jyuichiro  Okayama University, Graduate School of Medicine and Dentistry, Assistant Professor, 大学院・医歯学総合研究科, 講師 (80294403)
MOHRI Satoshi  Okayama University, Graduate School of Medicine and Dentistry, Research Associate, 大学院・医歯学総合研究科, 助手 (00294413)
Project Period (FY) 2001 – 2004
KeywordsSPring-8 / Physiome / Coronary Circulation / Cardiac Contraction / Crossbridge Dynamics / Intravital CCD / Transmural characteristics / Vascular regUlation
Research Abstract

Global dynamics of coronary circulation and cardiac contraction have been well documented in cardiology and circulatory physiology. Recently, transmural, i.e., from epicardial to endocardial heterogeneities have been getting more important for better understanding of the cardiac pathophysiology. Thus the objective of this study was to reveal the detailed transmural characteristics of the myocardial crossbridge dynamics and the coronary micro-circulation.
Firstly, we found that the diastolic predominant flow pattern was common throughout myocardial layers. During systolic phase, significant systolic reverse flow was observed in subendocardial arterioles, whereas a remarkable forward flow was observed in subepicardial arterioles. Intramural pressure gradient and myocardial stiffness (elastance) cause these transmural dissociations in systolic flow direction. We also found the vessel size dependent coronary vascular regulation system, and spatial and temporal myocardial flow heterogeneity using the SPring-8, custom-made intravital CCD microscopes, and molecular isotope tracers.
Secondly, we revealed the transmural difference of crossbridge dynamics using the SPring-8. During contraction phase, subepicardial and midcardial crossbridge dynamics were harmonized well each other and synchronized with the left ventricular pressure development. However the crossbridge of deeper myocardium decayed earlier than that of subepicardium during relaxation phase. Earlier relaxation in the myocardium in deeper regions seemed to be beneficial for diastolic blood inflow into deeper myocardium.
We are now going to promote the "Physiome" as the quantitative and integrated description of the functional behavior of the physiological state. We believe that the present studies contribute to the cardiovascular physiome in the sense that it clarified the close coupling between coronary microcirculation and actin-myosin macromolecular dynamics. (256/300 words)

  • Research Products

    (51 results)

All 2005 2004 2003 2002 2001

All Journal Article (46 results) Book (2 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Regional myocardial perfusion under exchange transfusion with liposomal hemoglobin : in vivo and in vitro studies using rat hearts.2005

    • Author(s)
      Matsumoto T
    • Journal Title

      Am J Physiol Heart Circ Physiol 288・4

      Pages: H1909-H1914

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Involvement of Aldosterone and Mineralocorticoid Receptors in Rat Mesangial Cell Proliferation and Deformability.2005

    • Author(s)
      Nishiyama A
    • Journal Title

      Hypertension 45・4

      Pages: 710-716

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Regression of capillary network in atrophied soleus muscle induced by hindlimb unweighting.2005

    • Author(s)
      Fujino H
    • Journal Title

      J Appl Physiol 98・4

      Pages: 1407-1413

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Beneficial effect of hydroxyfasudil, a specific Rho-kinase inhibitor, on ischemia/reperfusion injury in canine coronary microcirculation in vivo.2005

    • Author(s)
      Yada T
    • Journal Title

      J Am Coll Cardiol 45・4

      Pages: 599-607

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Exogenous NO suppresses flow-induced endothelium-derived NO production because of depletion of tetrahydrobiopterin.2005

    • Author(s)
      Mochizuki S
    • Journal Title

      Am J Physiol Heart Circ Physiol 288・2

      Pages: H553-H558

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Direct observation of epicardial coronary capillary hemodynamics during reactive hyperemia and during adenosine administration by intravital video2005

    • Author(s)
      Kiyooka T
    • Journal Title

      Am J Physiol Heart Circ Physiol 288・3

      Pages: H1437-H1443

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Normal distribution of ventricular pressure-volume area (PVA) of arrhythmic beats under atrial fibrillation in canine heart.2005

    • Author(s)
      Mohri S
    • Journal Title

      Am J Physiol Heart Circ Physiol 288・4

      Pages: H1740-H1746

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The Dynamics of Flow Velocities in Endocardial and Epicardial Coronary Arterioles.2005

    • Author(s)
      Toyota E
    • Journal Title

      Am J Physiol Heart Circ Physiol 288・4

      Pages: H1598-H1603

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Involvement of aldosterone and mineralocorticoid receptors in rat mesangial cell proliferation and deformity.2005

    • Author(s)
      A.Nishiyama
    • Journal Title

      Hypertension 45(4)

      Pages: 710-716

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Regression of capillary network in atrophied soleus muscle induced by hindlimb unweighting.2005

    • Author(s)
      H.Fujino
    • Journal Title

      J.Appl.Physiol. 98(4)

      Pages: 1407-1413

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Beneficial effect of hydroxyfasudil, a specific rho-kinase inhibitor, on ischemia/reperfusion injury in canine coronary microcirculation in vivo.2005

    • Author(s)
      T.Yada
    • Journal Title

      J.Am.Coll.Cardiol. 45(4)

      Pages: 599-607

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Exogenous nitric oxide suppresses flow-induced endothelium-derived nitric oxide production because of depletion of tetrahydrobiopterin.2005

    • Author(s)
      S.Mochizuki
    • Journal Title

      Am.J.Physiol. 288(2)

      Pages: H553-H558

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Direct observation of epicardial coronary capillary hemodynamics during reactive hyperemia and during adenosine administration by intravital videomicroscopy.2005

    • Author(s)
      T.Kiyooka
    • Journal Title

      Am.J.Physiol. 288(3)

      Pages: H1437-H1443

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Dynamics of Flow Velocities in Endocardial and Epicardial Coronary Arterioles.2005

    • Author(s)
      E.Toyota
    • Journal Title

      Am.J.Physiol. 288(4)

      Pages: H1598-H1603

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Normal distribution of ventricular pressure-volume area (PVA) of arrhythmic beats under atrial fibrillation in canine heart.2005

    • Author(s)
      S.Mohri
    • Journal Title

      Am.J.Physiol. 288(4)

      Pages: H1740-H1746

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Regional myocardial perfusion under exchange transfusion with liposomal hemoglobin : in vivo and in vitro studies using rat hearts.2005

    • Author(s)
      T.Matsumoto
    • Journal Title

      Am.J.Physiol. 288(4)

      Pages: H1909-H1914

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] X-ray diffraction from a left ventricular wall of rat heart.2004

    • Author(s)
      Yagi N
    • Journal Title

      Biophys J 86・4

      Pages: 2286-2294

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Sarcomere-length dependence of lattice volume and radial mass transfer of myosin cross-bridges in rat papillary muscle.2004

    • Author(s)
      Yagi N
    • Journal Title

      Pflugers Arch 448・2

      Pages: 153-160

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Direct observation and quantitative analysis of spatiotemporal dynamics of individual living monocytes during transendothelial migration.2004

    • Author(s)
      Hashimoto K
    • Journal Title

      Atherosclerosis 177・1

      Pages: 19-27

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Global heterogeneity of glomerular volume distribution in early diabetic nephropathy.2004

    • Author(s)
      Toyota E
    • Journal Title

      Kidney Int 66・2

      Pages: 855-861

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Load independence of temperature-dependent Ca^<2+> recirculation fraction in canine heart2004

    • Author(s)
      Mizuno J
    • Journal Title

      Jpn J Physiol 54・4

      Pages: 319-329

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Pattern differences between distributions of microregional myocardial flows in crystalloid- and blood-perfused rat hearts.2004

    • Author(s)
      Matsumoto T
    • Journal Title

      Am J Physiol 286・4

      Pages: H1331-H1338

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] X-ray diffraction from a left ventricular wall of rat heart.2004

    • Author(s)
      N.Yagi
    • Journal Title

      Biophysical J. 86(4)

      Pages: 2286-2294

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Sarcomere-length dependence of lattice volume and radial mass transfer of myosin cross-bridges in rat papillary muscle.2004

    • Author(s)
      N.Yagi
    • Journal Title

      Pflugers Arch. 448(2)

      Pages: 153-160

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Direct observation and quantitative analysis of spatiotemporal dynamics of individual living monocytes during transendothelial migration.2004

    • Author(s)
      K.Hashimoto
    • Journal Title

      Atherosclerosis 177(1)

      Pages: 19-27

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Global heterogeneity of glomerular volume distribution in early diabetic nephropathy.2004

    • Author(s)
      E.Toyota
    • Journal Title

      Kidney Int. 66(2)

      Pages: 855-861

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Load independence of temperature-dependent Ca^<2+> recirculation fraction in canine heart.2004

    • Author(s)
      J.Mizuno
    • Journal Title

      Jpn.J.Physiol. 54(4)

      Pages: 319-329

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Pattern differences between distributions of microregional myocardial flows in crystalloid- and blood-perfused rat hearts.2004

    • Author(s)
      T.Matsumoto
    • Journal Title

      Am.J.Physiol. 286(4)

      Pages: H1331-H1338

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] An X-ray diffraction study on contraction of rat papillary muscle with different afterloads.2003

    • Author(s)
      Okuyama H
    • Journal Title

      Adv Exp Med Biol 538

      Pages: 533-539

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Role of hyaluronic acid glycosaminoglycans in shear-induced endothelium-derived nitric oxide release.2003

    • Author(s)
      Mochizuki S
    • Journal Title

      Am J Physiol 285・2

      Pages: H722-H726

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] In vivo visualization of subendocardial arteriolar response in renovascular hypertensive hearts.2003

    • Author(s)
      Yada T
    • Journal Title

      Am J Physiol 284・5

      Pages: H1785-H1792

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Hydrogen peroxide, an endogenous endothelium-derived hyperpolarizing factor, plays an important role in coronary autoregulation in vivo.2003

    • Author(s)
      Yada T
    • Journal Title

      Circulation 107・7

      Pages: 1040-1045

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] An X-ray diffraction study on contraction of rat papillary muscle with different afterloads.2003

    • Author(s)
      H.Okuyama
    • Journal Title

      Adv.Exp.Med.Biol. 538 discussion 539

      Pages: 533-539

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] In vivo visualization of subendocardial arteriolar response in renovascular hypertensive hearts.2003

    • Author(s)
      T.Yada
    • Journal Title

      Am.J.Physiol. 284(5)

      Pages: H1785-H1792

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Role of hyaluronic acid glycosaminoglycans in shear-induced endothelium-derived nitric oxide release.2003

    • Author(s)
      S.Mochizuki
    • Journal Title

      Am.J.Physiol. 285(2)

      Pages: H722-H726

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Hydrogen peroxide, an endogenous endothelium-derived hyperpolarizing factor, plays an important role in coronary autoregulation in vivo.2003

    • Author(s)
      T.Yada
    • Journal Title

      Circulation 107(7)

      Pages: 1040-1045

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Measurements of endothelial cell-to-cell and cell-to-substrate gaps and micromechanical properties of endothelial cells during monocytes adhesion.2002

    • Author(s)
      Kataoka N
    • Journal Title

      Proc Natl Acad Sci USA 99・24

      Pages: 15638-15643

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Dynamic changes in three-dimensional architecture and vascular volume of transmural coronary microvasculature between diastolic- and systolic-arrested rat hearts.2002

    • Author(s)
      Toyota E
    • Journal Title

      Circulation 105・5

      Pages: 621-626

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Measurements of endothelial cell-to-cell and to-substrate gaps and micromechanical properties of endothelial cells during monocytes adhesion.2002

    • Author(s)
      N.Kataoka
    • Journal Title

      Proc.Natl.Acad.Sci.USA cell- 99(24)

      Pages: 15638-15643

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Dynamic changes in three-dimensional architecture and vascular volume of transmural coronary microvasculature between diastolic- and systolic-arrested rat hearts.2002

    • Author(s)
      E.Toyota
    • Journal Title

      Circulation 105(5)

      Pages: 621-626

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Microheterogeneity of myocardial blood flow. (Review)2001

    • Author(s)
      Matsumoto T
    • Journal Title

      Basic Res Cardiol 96・6

      Pages: 547-552

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] New double-tracer digital radiography for analysis of spatial and temporal myocardial flow heterogeneity.2001

    • Author(s)
      Matsumoto T
    • Journal Title

      Am J Physiol 280・1

      Pages: H465-H474

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Stenosis differentially affects subendocardial and subepicardial arterioles in vivo.2001

    • Author(s)
      Merkus D
    • Journal Title

      Am J Physiol 280・4

      Pages: H1674-H1682

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Microheterogeneity of myocardial blood flow.2001

    • Author(s)
      T.Matsumoto
    • Journal Title

      Basic Res.Cardiol. (Review) 96(6)

      Pages: 547-552

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] New double-tracer digital radiography for analysis of spatial and temporal myocardial flow heterogeneity.2001

    • Author(s)
      T.Matsumoto
    • Journal Title

      Am.J.Physiol 280(1)

      Pages: H465-H474

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Stenosis differentially affects subendocardial and subepicardial arterioles in vivo.2001

    • Author(s)
      D.Merkus
    • Journal Title

      Am.J.Physiol. 280(4)

      Pages: H1674-H1682

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 新・心臓病診療プラクティスシリーズ42005

    • Author(s)
      梶谷 文彦
    • Publisher
      文光堂(印刷中)
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 冠循環のバイオメカニクス2001

    • Author(s)
      梶谷文彦
    • Total Pages
      207
    • Publisher
      コロナ社
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 緑内障治療用房水排出インプラント2003

    • Inventor(s)
      毛利 聡他
    • Industrial Property Rights Holder
      国立大学法人岡山大学
    • Industrial Property Number
      特願2004-185444
    • Filing Date
      2003-06-23
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] pH測定装置及びpH測定方法2003

    • Inventor(s)
      毛利 聡他
    • Industrial Property Rights Holder
      国立大学法人岡山大学
    • Industrial Property Number
      特願2004-319762
    • Filing Date
      2003-11-02
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] 静脈内注入単一ネフロン機能診断用薬剤2001

    • Inventor(s)
      山本 徳則他
    • Industrial Property Rights Holder
      科学技術振興事業団
    • Patent Publication Number
      特開2003-171318
    • Filing Date
      2001-12-03
    • Acquisition Date
      2003-06-20
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2006-07-11  

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