骨転移研究のためのマイクロ流体環流システムを用いた３次元骨髄微小環境の構築

2014 Fiscal Year Annual Research Report

• Project/Area Number: 13F03049
• Research Institution: The University of Tokyo
• Principal Investigator: 竹内 昌治 東京大学, 生産技術研究所, 教授 (90343110)
• Co-Investigator(Kenkyū-buntansha): HSIAO Amy 東京大学, 生産技術研究所, 外国人特別研究員
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 3D cell culture / Tissue engineering / Cancer cell / Microfluidic / Mesenchymal stem cells / Cell fiber

Outline of Annual Research Achievements

The main purpose of this research is to engineer the bone marrow niche microenvironment in vitro for metastatic cancer studies using micro-technologies. In the original plan, we proposed to include cancer cells, osteoblasts, and endothelial cells in the system. During the process, we learned about the importance of mesenchymal stem cells and pursued to create three-dimensional (3D) microenvironments from mesenchymal stem cells (MSCs). Initially, multipotent dedifferentiated fat (DFAT) cells that possess similar differentiation abilities as MSCs were used. DFAT cells were encapsulated in the core region of alginate shell fibers using a microfluidic double co-axial device to make 3D cell fiber constructs and differentiated into the smooth muscle cell lineage. Subsequently MSCs derived from adipose tissue, adipose-derived stem cells (ADSCs), were cultured as cell fiber constructs using the same cell fiber technology and differentiated into adipocytes. The conditioned media from the differentiated ADSC fibers was further used to culture MCF-7 breast cancer cells to study its effect on breast cancer cell proliferation.

Research Progress Status

26年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

26年度が最終年度であるため、記入しない。

Remarks

【Award】2014 FAST: Functional Analysis & Screening Technologies Congress Poster Award

Research Products

• [Journal Article] Smooth muscle-like tissue constructs with circumferentially oriented cells formed by the cell fiber technology. (2015)
  • Author(s): Hsiao AY, Okitsu T, Onoe H, Kiyosawa M, Teramae H, Iwanaga S, Kazama T, Matsumoto T, Takeuchi S.
  • Journal Title: PLoS ONE Volume: 10(3) Pages: 1-16
  • DOI: 10.1371/journal. pone.0119010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Human adipose-derived stem cell fiber for breast reconstruction. (2015)
  • Author(s): Hsiao AY, Okitsu T, Takeuchi S.
  • Organizer: IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
  • Place of Presentation: Estoril, Portugal
  • Year and Date: 2015-01-20 – 2015-01-20
• [Presentation] Cell fiber technology for engineering functional 3D models. (2014)
  • Author(s): Hsiao AY, Onoe H, Okitsu T, Kato-Negishi M, Takeuchi S.
  • Organizer: FAST: Functional Analysis & Screening Technologies Congress
  • Place of Presentation: Boston, USA
  • Year and Date: 2014-11-17 – 2014-11-17