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2014 Fiscal Year Annual Research Report

生体様の代謝と輸送を再現する肝組織マイクロデバイス

Research Project

Project/Area Number 13F03713
Research InstitutionThe University of Tokyo

Principal Investigator

酒井 康行  東京大学, 生産技術研究所, 教授 (00235128)

Co-Investigator(Kenkyū-buntansha) PERRY Guillaume  東京大学, 生産技術研究所, 外国人特別研究員
Project Period (FY) 2013-04-01 – 2015-03-31
Keywordshepatocyte / microfludic / metabolism / transport
Outline of Annual Research Achievements

To realize hepatocytes-based assays exhibiting many biological functions, we investigated the feasibilities of microwell device and microfluidic system for better metabolism and future bile recovery. In the case of the microwell device, we made a honeycomb network of pyramidal microwells to create small aggregates. In addition, we optimized the microenvironment by the overlay of Matrigel or a liver sinusoidal endothelial cell line. Although we successfully formed stably-attached aggregates, we were unfortunately not able to control precisely the formation of bile pocket in the center of the microwell. We therefore decided to change from microwell to "virtual microwell", namely proteins patterning on PDMS surface. It is on-going at this moment. As an another approach, we changed the design of previous our devices (Nakao et al., Biomicrofluidics, 5, 22212, 2011) to increase the diffusion between the culture medium and cells. To be able to monitor several devices at the time, we made a setup of 8 parallel devices to decrease the flow rate. Cell inoculation to the culture area was successfully checked in real-time. We tried different design and conditions and finally were able to fill the culture area and to get two lines of fixed hepatocytes. However, we have not yet succeeded completely when we use freshly-isolated cells at this moment. The experiments are also on-going now.

Research Progress Status

26年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

26年度が最終年度であるため、記入しない。

  • Research Products

    (4 results)

All 2015 2014

All Presentation (4 results) (of which Invited: 1 results)

  • [Presentation] Liver cord reconstruction in microfluidic device for drug screening by bile recovery : 1st steps2015

    • Author(s)
      Perry, G., Xiao, W., Provin, C., Shinohara, M., Fujii, T. and Sakai, Y
    • Organizer
      Symposium on New Technology for Cell-Based Drug Assay
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2015-01-13
  • [Presentation] Establishment of a multicellular 3D liver model by co-culturing rat hepatocytes with TMNK-1 based on gas-permeable membranes for drug screening2015

    • Author(s)
      Xiao, W., Perry, G., Komori, K. and Sakai, Y
    • Organizer
      Symposium on New Technology for Cell-Based Drug Assay
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2015-01-13
  • [Presentation] Establishment of a multicellular 3D liver model by co-culturing rat hepatocytes with TMNK-1 based on gas-permeable membranes for drug screening2014

    • Author(s)
      Xiao, W., Perry, G., Komori, K. and Sakai, Y.
    • Organizer
      27th Meeting of the Japanese Society for Alternatives to Animal Experiments
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2014-12-05 – 2014-12-07
  • [Presentation] 細胞・組織培養における生理学的酸素供給2014

    • Author(s)
      酒井康行
    • Organizer
      第12回 がんとハイポキシア研究会
    • Place of Presentation
      佐賀
    • Year and Date
      2014-11-21 – 2014-11-22
    • Invited

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Published: 2016-06-01  

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