2014 Fiscal Year Annual Research Report
生体様の代謝と輸送を再現する肝組織マイクロデバイス
| Project/Area Number |
13F03713
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| Research Institution | The University of Tokyo |
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Principal Investigator |
酒井 康行 東京大学, 生産技術研究所, 教授 (00235128)
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| Co-Investigator(Kenkyū-buntansha) |
PERRY Guillaume 東京大学, 生産技術研究所, 外国人特別研究員
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | hepatocyte / microfludic / metabolism / transport |
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| Outline of Annual Research Achievements |
To realize hepatocytes-based assays exhibiting many biological functions, we investigated the feasibilities of microwell device and microfluidic system for better metabolism and future bile recovery. In the case of the microwell device, we made a honeycomb network of pyramidal microwells to create small aggregates. In addition, we optimized the microenvironment by the overlay of Matrigel or a liver sinusoidal endothelial cell line. Although we successfully formed stably-attached aggregates, we were unfortunately not able to control precisely the formation of bile pocket in the center of the microwell. We therefore decided to change from microwell to "virtual microwell", namely proteins patterning on PDMS surface. It is on-going at this moment. As an another approach, we changed the design of previous our devices (Nakao et al., Biomicrofluidics, 5, 22212, 2011) to increase the diffusion between the culture medium and cells. To be able to monitor several devices at the time, we made a setup of 8 parallel devices to decrease the flow rate. Cell inoculation to the culture area was successfully checked in real-time. We tried different design and conditions and finally were able to fill the culture area and to get two lines of fixed hepatocytes. However, we have not yet succeeded completely when we use freshly-isolated cells at this moment. The experiments are also on-going now.
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| Research Progress Status |
26年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
26年度が最終年度であるため、記入しない。
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[Presentation] Liver cord reconstruction in microfluidic device for drug screening by bile recovery : 1st steps2015
Author(s)
Perry, G., Xiao, W., Provin, C., Shinohara, M., Fujii, T. and Sakai, Y
Organizer
Symposium on New Technology for Cell-Based Drug Assay
Place of Presentation
Tokyo, Japan
Year and Date
2015-01-13
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