• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2005 Fiscal Year Annual Research Report

哺乳類卵胞の選択的死滅制御機構とその人為支配による潜在的卵巣卵の利用

Research Project

Project/Area Number 13GS0008
Research InstitutionThe University of Tokyo

Principal Investigator

眞鍋 昇  東京大学, 大学院農学生命科学研究科, 教授 (80243070)

Co-Investigator(Kenkyū-buntansha) 宮野 隆  神戸大学, 農学部, 教授 (80200195)
酒巻 和弘  京都大学, 生命科学研究科, 助教授 (20271017)
若山 照彦  理化学研究所, 神戸研究所・発生再生科学総合研究センター, チームリーダー(研究職) (40360672)
Keywords哺乳類 / 卵巣 / 卵胞 / 選択的死滅 / アポトーシス / 細胞死受容体 / 細胞内シグナル伝達阻害因子 / 異種移植
Research Abstract

脊椎動物は多様な生殖戦略をもつ。魚類の多くは数万〜数億のなかで幸運で優れた仔だけが生き残る。逆に、哺乳類は性周期毎に1〜10個の卵母細胞を排卵し、極わずかの仔を産み、それを大切に育てる。成熟した哺乳類卵巣には、胎児期に減数分裂の途中・ディプロテン期で休止した5〜100万個の卵母細胞が含まれ、これが性周期毎に発育・成熟して排卵に至る。この過程で、最終的に排卵にいたるものの100倍以上の卵胞が発達を開始し、99%以上が選択的に死滅する。この卵胞の選択的死滅は優秀で強靭な子孫を残す戦略として重要であるが、これを調節している分子制御機構は未解明である。本研究は、哺乳類卵巣において繰返される卵胞の選択的死滅を支配している分子機構を解明し、これをもとに細胞死を制御している遺伝子の発現をin vivo制御することで卵胞発育を人為的に支配しようとするものである。この新技術で、食肉処理場で捨てられる卵巣内の卵母細胞の有効利用を実現しようとしている。今年度は実証的研究に注力した。卵胞顆粒層細胞に特異的に発現している新規細胞死受容体を認識するユニークなモノクロナル抗体を用いてデコイ受容体を同定し、その発現動態を明らかとした。さらに細胞死受容体を介する細胞内アポトーシスシグナル伝達系を担うカスパーゼ系を阻害するcFLIPを見いだして遺伝子とアミノ酸配列を決定した。これの発現を腫瘍壊死因子α調節し、その制御に多面的機能をもつinterleukin-6が重要に関わっていることを示唆した。併行して、大型家畜の未成熟な卵胞を含む卵巣片を免疫不全マウス腎漿膜嚢に異種移植し、in vivo遺伝子導入とRNA発現阻止(iRNA)にて人為的に制御できるシステムを開発した。

  • Research Products

    (43 results)

All 2006 2005

All Journal Article (38 results) Book (5 results)

  • [Journal Article] Erythropoietion-producing cells in the liver of ICR-derived glomerulonephritis (ICGN)mice.2006

    • Author(s)
      Misuzu Yamaguchi-Yamada, Naotsugu Akashi, Yasufumi Goto, Sayuri Anan, Yoshie Yamamoto, Atsuo Ogura, Noboro Manabe
    • Journal Title

      The Journal of Veterinary Medical Science 68

      Pages: 65-68

  • [Journal Article] Propagation of an infertile hermaphrodite mouse lacking germ cells, using nuclear transfer and embryonic stem cell technology.2005

    • Author(s)
      Wakayama S, Kishigami S, Van Thuan N, Ohta H, Hikishi T, Mizutani E, Yanagimashi R, Wakayama T.
    • Journal Title

      Proceeding of National Academic Sciences in USA 102

      Pages: 29-33

  • [Journal Article] Transplantation of spermatogonial stem cells isolated from leukemic mice restores fertility without inducing leukemia.2005

    • Author(s)
      Fujita, K., Ohta, H., Thujimura, A., Takao, T., Miyagawa, Y., Takada, S., Matsumiya, K., Wakayama, T., Okuyama,A.
    • Journal Title

      Journal of Clinical Invesigation 115

      Pages: 1855-1861

  • [Journal Article] Generation of progeny from embryonic stem cells by microinsemination of male germ cells from chimeric mice.2005

    • Author(s)
      Mizutani E, Ohta H, Kishigami S, Van Thuan N, Hikichi T, Wakayama S, Sato E, Wakayama T.
    • Journal Title

      Genesis 43

      Pages: 34-42

  • [Journal Article] Changes in expression of anti-apoptotic protein, cFLIP,in granulosa cells during follicular atresia in porcine ovaries.2005

    • Author(s)
      Fukuo Matsuda-Minehata, Yasufumi Goto, Naoko Inoue, Noboru Manabe
    • Journal Title

      Molecular Reproduction and development 72

      Pages: 145-151

  • [Journal Article] Birth of Offispring after Transfer of Mongolian Gerbil(Meriones unguiculatus)Embryos Cryopreserved by Vitrification.2005

    • Author(s)
      Mochida K, Wakayama T, Takano K, Noguchi Y, Yamamoto Y, Suzuki O, Matsuda J, Ogura A
    • Journal Title

      Molecular Reproduction and Development 70

      Pages: 464-470

  • [Journal Article] Effects of long-term grafting on follicular growth in porcine ovarian cortical grafts xenoplanted to severe combined immunodeficient(SCID)mice.2005

    • Author(s)
      Noriko Kagawa, Yohichiro Sakurai, Takashi Miyano, Noboru Manabe
    • Journal Title

      Journal of Reproduction and Devevelopment 51

      Pages: 77-85

  • [Journal Article] Ovarian activity and oocyte development during follicular development in pigs at different reproductive phases estimated by the repeated endoscopic method.2005

    • Author(s)
      Jozsef Ratky, Helmut Torner, Istvan Egerszegi, Falk Schneider, Peter SarlOs, Noboru Manabe, Klaus-Peter Brussow
    • Journal Title

      Journal of Reproduction and Devevelopment 51

      Pages: 253-263

  • [Journal Article] Conflict of estrogenic activity by various phthalates between in vitro and in vivo models related to the expression of Calbindin-D_<9K+>2005

    • Author(s)
      Eui-Ju Hong, Youn-Kyu Ji, Kyung-Chul Choi, Noboro Manabe, Eui-Bae Jeung
    • Journal Title

      Journal of Reproduction and Devevelopment 51

      Pages: 253-263

  • [Journal Article] Effects of in utero exposure to bisphenol A on mRNA expression of arylhydrocarbon and retinoid receptors in murine embryos.2005

    • Author(s)
      Hanako Nishizawa, Maki Morita, Miki Sugimoto, Stoshi Imanishi, Noboru Manabe
    • Journal Title

      Journal of Reproduction and devevelopement 51

      Pages: 315-324

  • [Journal Article] Comparison of follicular and oocyte development and reproductive hormone secretion during the ovulatory period in hungarian native breed,Mangalica,and Landrace gilts.2005

    • Author(s)
      Jozsef Ratky, Klaus-Peter Brussow, Istvan Egerszegi, Hwlmut Torner, Falk Schneider, Laszlo Solti, Noboru Manabe
    • Journal Title

      Journal of Reproduction and devevelopement 51

      Pages: 427-432

  • [Journal Article] Effects of exposure in utero to bisphenol A on the expression of arylhydrocarbon receptor,related factors and xenobiotic metabolizing enzymes in murine embryos.2005

    • Author(s)
      Hanako Nishizawa, Satoshi Imanishi, Noboru Manabe
    • Journal Title

      Journal of Reproduction and devevelopement 51

      Pages: 593-605

  • [Journal Article] Chenges in expression and localization of connexin 43 mRNA and protein in porcine ovary granulosa cells during follicular atresia.2005

    • Author(s)
      Yuan Cheng, Naoko Inoue, Fuko Matsuda-Minehata, Yasufumi Goto, Akihisa Maeda, Noboru Manabe
    • Journal Title

      Journal of Reproduction and devevelopement 51

      Pages: 627-637

  • [Journal Article] Growth and maturation of porcine follicles and oocytes following xenotransplantation of ovarian tissues and in vitro maturation culture.2005

    • Author(s)
      Noriko Kagawa, Takashi Miyano, Noboru Manabe
    • Journal Title

      Journal of Reproduction and devevelopement 51

      Pages: 741-748

  • [Journal Article] In vitro growth of mammalian oocytes.2005

    • Author(s)
      Miyano T.
    • Journal Title

      Journal of Reproduction and devevelopement 51

      Pages: 169-176

  • [Journal Article] Transfroming growth factor-β1 mediated up-regulation of lysyl oxidase in the kidneys of hereditary nephrotic mouse with chronic renal fibrosis.2005

    • Author(s)
      Yasufumi Goto, kozue Uchio-Yamada, Anan Sayuri, Yoshie Yamamoto, Atsuo Ogura, Noboru Manabe
    • Journal Title

      Virchowx Archiv 447

      Pages: 859-868

  • [Journal Article] Decreased expression of matrix metalloproteinases and tissue inhibitors of metalloproteinase in the kidneys of hereditary nephrotic(ICGN)mice.2005

    • Author(s)
      Kozue Uchio-Yamada, Noboru Manabe, Yasufumi Goto, Sayuri Anann, Yoshie Yamamoto, Kaoru Takano, Atsuo Ogura, Junichiro Matsuda
    • Journal Title

      The Journal of Veterinary Medical Science 67

      Pages: 35-41

  • [Journal Article] Dysfunction of erythropoietin-producing interstitial cells in the kidneys of ICR-derived glomerulonephritis (ICGN)mice.2005

    • Author(s)
      Misuzu Yamaguchi-Yamada, Noboru Manabe, Minako Kiso, Yasufumi Goto, Toshiharu Mori, Chinatsu Sakata, Sayuri Anan, Masaya Nagao, Yoshie Yamamoto, Atsuo Ogura
    • Journal Title

      The Journal of Veterinary Medical Science 67

      Pages: 891-899

  • [Journal Article] Effect of human erythropoietin (hEPO)treatment on anemia in ICR-derived glomerulaonephrosis(ICGN)mice.2005

    • Author(s)
      Yohei Miyamoto, Keiko Kuramitsu-Miyamoto, Ema Iwanaga, Kozue Uchio-Yamada, Misuzu Yamaguchi-Yamada, Atsuo Ogura, Noboru Manabe
    • Journal Title

      Experimental Animal 54

      Pages: 181-184

  • [Journal Article] Disposition of disphenol A in pregnant mice and fetuses after a single and repeated oral administration.2005

    • Author(s)
      Yuko Kawamoto, Wakoto Matsuyama, Masahiro Morikawa, Maki Morita, Miki Sugimoto, Moboru Manabe, Shinsuke Morisawa
    • Journal Title

      Toxicological and Environmental Chemistry 87

      Pages: 199-213

  • [Journal Article] Effects of dietary supplementation with branched-chain amino acids(BCAAs)during nursing on plasma BCAA levels and subsequent growth in cattle.2005

    • Author(s)
      Jun-You Li, Kazumi Suzuki, Yukihiro Koike, DS Chen, Tomohiro Yonezawa, Masugi Nishihara, Noboru Manabe
    • Journal Title

      Asian Australian Journal of Animal Science 18

      Pages: 1440-1444

  • [Journal Article] New preservation method for mouse spermatozoa without freezing.2005

    • Author(s)
      Van Thuan N, Wakayama S, Kishigami S, Wakayama T
    • Journal Title

      Biology of Reproduction 72

      Pages: 444-450

  • [Journal Article] Establishment of male and female nuclear transfer embryonic stem cell lines from different mouse strains and tissues.2005

    • Author(s)
      Wakayama S, Ohta H, Kishigami S, Van Thuan N, Hikichi T, Mizutani E, Miyake M, Wakayama T
    • Journal Title

      Biology of Reprodution 72

      Pages: 932-936

  • [Journal Article] Changes in expression of anti-apoptotic protein, cFLIP, in granulose cells during follicular atresia in porcine ovaries.(review)2005

    • Author(s)
      Noboru Manabe, Fuko Matsuda-Minehata, Yasufumi Goto, Akihisa Maeda, Yuan Cheng, Takafumi Sai, Miyuki Kohori, Maiko Endo, Junyou Li
    • Journal Title

      European Journal of Cellular and Molecular Physiopathology 44

      Pages: 120-124

  • [Journal Article] Anti-apoptotic activity of porcine cFLIP in ovarian grabulosa cell lines.(review)2005

    • Author(s)
      Noboru Manabe, Fuko Matsuda-Minehata, Yasufumi Goto, Akihisa Maeda, Yuan Cheng, Takafumi Sai, Miyuki Kohori, Maiko Endo, Junyou Li
    • Journal Title

      European Journal of Cellular and Molecular Physiopathology 44

      Pages: 125-129

  • [Journal Article] Molecular cloning of porcine; cellular flice-like inhibitory protein.(review)2005

    • Author(s)
      Noboru Manabe, Fuko Matsuda-Minehata, Yasufumi Goto, Akihisa Maeda, Yuan Cheng, Takafumi Sai, Miyuki Kohori, Maiko Endo, Junyou Li
    • Journal Title

      European Journal of Biology and Chemistry of Proteins 18

      Pages: 201-204

  • [Journal Article] Changes in the expression of bid in granulosa cells during follicular atresia in pig ovaries.(review)2005

    • Author(s)
      Noboru Manabe, Fuko Matsuda-Minehata, Yasufumi Goto, Akihisa Maeda, Yuan Cheng, Takafumi sai, Miyuki Kohori, Maiko Endo, Junyou Li
    • Journal Title

      European Journal of Biology and Chemistry of Proteins 18

      Pages: 205-208

  • [Journal Article] Characteristics of initiation and early events for muscle development in the Xenopus limb bud.2005

    • Author(s)
      Satoh A., Sakamaki K., Ide H., Tamura K.
    • Journal Title

      Developmenta Dynamics 234

      Pages: 846-857

  • [Journal Article] Expression and functional analysis of genes deregulated in mouse placental over growth models : Car2 and Ncaml.2005

    • Author(s)
      Singh U, Sun T, Shi W, Schulz R, Nuber UA, Varanou A, Hemberger MC, Elliott RW, Ohta H, Wakayama T, Fundele R
    • Journal Title

      Developmenta Dynamics 234

      Pages: 1034-1045

  • [Journal Article] Germinal vesicle materials are requisite for male pronucleus formation but not for change in the activities of CDKI and MAP kinase during maturation and fertilization of pig oocytes2005

    • Author(s)
      Ogushi S, Fulka J Jr, Miyano T
    • Journal Title

      Developmental Biology 286

      Pages: 287-298

  • [Journal Article] Generation of normal progeny by intracytoplasmic sperm injection following grafting of testicular tissue from cloned mice that died postnatally.2005

    • Author(s)
      Ohta H, Wakayama T
    • Journal Title

      Biology of Reproduction 73

      Pages: 390-395

  • [Journal Article] Interleukin-6 regulates the expression of cellular FLICE-like inhibitory protein in granulose cells of ovaries.(abstrqact)2005

    • Author(s)
      Akihisa Maeda, Yasufumi goto, Fuko Matsuda-Minehata, Yuan Cheng, Takafumi Sai, Noboru Manabe
    • Journal Title

      Biology of Reproduction 70

      Pages: 115-116

  • [Journal Article] Granulosa cell is a typr II apoptotic cell that regulates follicular atresia in pig ovaries.(abstrqact)2005

    • Author(s)
      Takafumi Sai, Toshikatsu Matsui, Yasufumi Goto, Fuko Matsuda-Minehata, Naoko Inoue, Akihisa Maeda, Yuan Cheng, Noboru Manabe
    • Journal Title

      Biology of Reproduction 70

      Pages: 116-117

  • [Journal Article] CFLIP regulates granulose cell apoptosis during follicular atresia in porcine ovaries.(abstrqact)2005

    • Author(s)
      Fuko Matsuda-Minehata, Yasufumi Goto, Naoko Inoue, Noboru Manabe
    • Journal Title

      Biology of Reproduction 70

      Pages: 118-119

  • [Journal Article] Apoptosis is regulated by soluble Fas during luteolysis in Iuteal cells of mouse ovaries.(abstrqact)2005

    • Author(s)
      Noboru Manabe, Kohji Komatsu, Minako Kiso, Yasufumi Goto, Sayuri Anan, Junyou Li
    • Journal Title

      Biology of Reproduction 70

      Pages: 122-123

  • [Journal Article] Connexin-43 has relation to regulation of granulosa cell apoptosis during atresia in pig ovarian follicules. (abstrqact)2005

    • Author(s)
      Yuan Cheng, Naoko Inoue, Fuko Matsuda-Minegishi, Yasufumi Goto, Akihisa Maeda, Junyou Li, Noboru Manabe
    • Journal Title

      Biology of Reproduction 70

      Pages: 124-125

  • [Journal Article] Changes in the expression of Bid in granulosa cells during follicular atresia in pig ovaries.(abstrqact)2005

    • Author(s)
      Naoko Inoue, Ryosuke Yoshioka Yasufumi Goto, Fuko Matsuda-Minehata, Akihisa Maeda, Yuan Cheng, Noboru Manabe
    • Journal Title

      Biology of Reproduction 70

      Pages: 223-224

  • [Journal Article] Round spermatids stained with mitotracker can be used to produce offspring more simply2005

    • Author(s)
      Hikichi T, Kishigami S, Thuan NV, Ohta H, Mizutani E, Wakayama S, Wakayama T.
    • Journal Title

      Zygote 13

      Pages: 55-61

  • [Book] Gamete Biology-Emerging Frontiers in Fertility and Contraceptive Development : Oocyte growth and acquisition of meiotic competence.2006

    • Author(s)
      Miyano T, Manabe N.
    • Total Pages
      312
    • Publisher
      Nottinghm University Press
  • [Book] Endocrine Disrupting Chemicals : Efect of various phtalates on the induction of Calbindin-D9k as an estrogen-like chemical in in vitro ans in vivo models.2005

    • Author(s)
      Eui-Ju Hong, Youn-Kyu Ji, Kyung-Chul Choi, Noboru Manabe, Eui-Bae Jeung
    • Total Pages
      482
    • Publisher
      The Endocrine Society
  • [Book] ウシの解剖・消化器系2005

    • Author(s)
      眞鍋 昇
    • Total Pages
      138
    • Publisher
      チクサン出版株式会社
  • [Book] 犬の繁殖(Dog Breeding)2005

    • Author(s)
      眞鍋 昇, 佐籐英明, 永村武美
    • Total Pages
      338
    • Publisher
      株式会社インターズー
  • [Book] 卵子形成・成熟および排卵の機能 : 生命の誕生に向けて2005

    • Author(s)
      宮野 隆
    • Total Pages
      482
    • Publisher
      近代出版株式会社・日本哺乳動物卵子学会

URL: 

Published: 2008-05-08   Modified: 2014-10-08  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi