• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2014 Fiscal Year Annual Research Report

DNAナノテクノロジーを基盤とした生体分子反応の直接1分子観察と応用

Research Project

Project/Area Number 13J01399
Research InstitutionKyoto University

Principal Investigator

楊 泱泱  京都大学, 理学研究科, 特別研究員(PD)

Project Period (FY) 2013-04-01 – 2015-03-31
KeywordsDNA origami / rotaional nanodevice / photocontrollable / azobenzene
Outline of Annual Research Achievements

(1) Remotely controllable DNA nanocapsule for the delivery of protein by photo regulation.
A 70 nm × 10 nm × 10 nm 3D DNA capsule was designed and constructed, which containing a small nanospace inside employed for carrying target molecules. The nanostructure has been successfully assembled and confirmed by AFM. Here caspase-3 was chosen as target for delivery, which can induce the apoptosis of cancer cells. The expression of the protein has been successfully carried out and the activity also confirmed.The caspase-3 labeled nanocapsule will be further introduced into the cell system. The photoinduced cleavage of caged nanocapsule has been examined by AFM before and after the labeling.
(2) Direct visualization of rotational motions on a single DNA origami
A novel nanomechanical device based on DNA origami was developed by empoying DNA photoswitches. A rectangular DNA origami assembled with two double-crossover molecules connected by a rigid linker: a short nucleic acid strand modified with oligophenylene-ethylene, in which one of the DX molecules was located on the surface of DNA tile as a pointer. The nanostructures were constructed and confirmed by AFM. Two pairs of photoresponsive oligonucleotides containing azobenzene molecules were introduced into DX pointer and DNA tile scaffold separately. By switching the photoirradiation with different wavelengths, the DX pointer can be driven to rotate on the DNA tile in a pointer-on-a clock fashion. Moreover, reversible rotational movement of DX pointer on single DNA tile was directly visualized using high-speed AFM.

Research Progress Status

26年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

26年度が最終年度であるため、記入しない。

  • Research Products

    (5 results)

All 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 3 results) Remarks (2 results)

  • [Journal Article] Direct Observation of the Dual-Switching Behaviours Corresponding to the State Transition in a DNA Origami2014

    • Author(s)
      Yang, Y.; Endo, M.; Suzuki, Y.; Hidaka, K.; Sugiyama, H.
    • Journal Title

      Chemical Communication

      Volume: 50 Pages: 4211-4213

    • DOI

      10.1039/C4CC00489B

    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Photoresponsive DNA Nanocapsule Having an Open/Close System for Capture and Release of Nanomaterials2014

    • Author(s)
      Takenaka, T.; Endo, M.; Suzuki, Y.; Yang, Y.; Emura, T.; Hidaka, K.; Kato, T.; Mitata, T.; Namba, K.; Sugiyama, H
    • Journal Title

      Chemistry - A European Journal

      Volume: 20 Pages: 14951-14954

    • DOI

      10.1002/chem.201404757

    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Dynamic Assembly/Disassembly Processes of Photoresponsive DNA Origami Nanostructures Directly Visualized on a Lipid Membrane Surface2014

    • Author(s)
      Suzuki, Y.; Endo, M.; Yang, Y.; Sugiyama, H.
    • Journal Title

      Journal of the American Chemical Society

      Volume: 136 Pages: 1714-1717

    • DOI

      10.1021/ja4109819

    • Peer Reviewed / Acknowledgement Compliant
  • [Remarks] Sugiyama Laboratory Homepage

    • URL

      http://kuchem.kyoto-u.ac.jp/chembio/

  • [Remarks] WPI-iCeMS DNA nanotechnology group

    • URL

      http://kuchem.kyoto-u.ac.jp/chembio/top_page_e.html

URL: 

Published: 2016-06-01  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi