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2005 Fiscal Year Final Research Report Summary

Study on the early process (especially adhesive and invasive process) of pathogenesis of bacterial enteropathogens and its cellular response

Research Project

Project/Area Number 14021055
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionOsaka University

Principal Investigator

HONDA Takeshi  Osaka University, Research Institute for Microbial Diseases, Professor, 微生物病研究所, 教授 (60029808)

Project Period (FY) 2002 – 2005
Keywordsenteropathogenic E coli / Vibrio parahaemolyticus / Providencia alcalifaciens / Type 3 secretion system / effector / EHEC / Cortactin
Research Abstract

(1) Result on enteropathogenic E. coli (EPEC, EHEC) : We found that cortactin and talin bind to Tir, an effector injected vir Type III secretion system (TTSS) and the binding is essential for causing attaching and effacing lesion formed by EPEC and EHEC. We also found that EspB forming tip of needle of TTSS also bind host molecule of a catenin which is essential for the A/E lesion formation.
(2) Result on Vibrio parahaemolyticus : The most important virulence factor of this organism is TDH../TRH (an exotoxin), however, this may not be enough to full understand of pathogenesis. Whole genome analysis completed suggested us that this has two sets of TTSSs. TTSS-1 is present in large chromosome and secrete an effector having apoptotic cytotoxicity. On the other hand, TTSS-2 found in only clinical isolates is locate on small chromosome and unidentified effector which may have enteropathogenicity may be imvolved.
(3) Result on Providencia alcalifaciens : We have found, for the first time, an outbreak attributed to P alcalifaciens (PA). We also found that this organism possess TTSS on a large plasmid.. As shown above and unlike our speculation that these 3 pathogens may have different mechanism for causing diseases, it became clear that the 3 pathogens possess a common process, TTSS, which may serve as one of the important virulence factor.

  • Research Products

    (12 results)

All 2006 2005 2004 2003

All Journal Article (12 results)

  • [Journal Article] Cortactin is essential for F-actin assembly in enteropathogenic Escherichia coli(EHEC)- and enterohaemorrhagic E. coli (EHEC)- induced pedestals and the α-helical region is involved in the localization of cortactin to bacterial attachment sites.2006

    • Author(s)
      Vlademir.C., T.Kodama, N.Nijjstad, S.K.Abolghait, T.Iida, T.Honda
    • Journal Title

      Cellular Microbiology 8(5)

      Pages: 769-780

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification of proteins secreted via Vibrio parahaemolyticus TypeIII secretion system 1.2006

    • Author(s)
      Ono.T., K.S.Park, M.Ueta, T.Iida, T.Honda
    • Journal Title

      Infect. Immun. 74(2)

      Pages: 1032-1042

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cortactin is essential for F-actin assembly in enteropathogenic Escherichia coli(EHEC)-and enterohaemorrhagic E. coli (EHEC)-induced pedestals and the a-helical region is involved in the localization of cortactin to bacterial attachment sites.2006

    • Author(s)
      Vlademir.C., T.Kodama, N.Nijjstad, S.K.Abolghait, T.Iida, T.Honda.
    • Journal Title

      Cellular Microbiology 8(5)

      Pages: 769-780

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Identification of proteins secreted via Vibrio parahaemolyticu. Typal secretion system 1.2006

    • Author(s)
      uno.L, K.J.rarx, M.u eta, L naa ana 1.tionaa.
    • Journal Title

      Infect. Immun. 74(2)

      Pages: 1032-1042

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Microarray analysis identifies apoptosis regulatory gene expression in HCT116 cells infected with thermostable direct hemolysin-deletion mutant of Vibrio parahaemolyticus.2005

    • Author(s)
      Rabindra.N.Bhattachariee, K.S.Park, K.Okada, Y.Kumagai, S.Uematsu, O.Takeuchi, S.Akira, T.Iida, T.Honda
    • Journal Title

      BBRC 335

      Pages: 328-334

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] vpaH, a gene encoding a novel histone-like nucleoid structure-like protein that was possibly horizontally acquired, regulates the Vibrio parahaemolyticus TH3996.2005

    • Author(s)
      Park, K.S., M.Arita, T.Iida, T.Honda
    • Journal Title

      Infect. Immun. 73(9)

      Pages: 5754-5761

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Microarray analysis identifies apoptosis regulatory gene expression in HCT116 cells infected with thermostable direct hemolysin-deletion mutant of Vibrio parahaemolyticus.2005

    • Author(s)
      Rabindra.N.Bhattachariee, K.S.Park, K.Okada, Y.Kumagai, S.Uematsu, 0.Takeuchi, S.Akira, T.Iida, T.Honda.
    • Journal Title

      BBRC 335

      Pages: 328-334

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] vpaH, a gene encoding a novel histone-like nucleoid structure-like protein that was possibly horizontally acquired, regulates the Vibrio rsrirnhrromelh)firTHie TI-110012005

    • Author(s)
      Park, K.S., M.Arita, T.Iida., T.Honda
    • Journal Title

      infect. immun. 73(9)

      Pages: 5754-5761

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Functional characterization of two TypeIII secretion systems of Vibrio parahaemolyticus.2004

    • Author(s)
      K.S.Park, T.Ono, M.Rokuda, M.H.Jang, K.Okada, T.Iida, T.Honda
    • Journal Title

      Infect. Immun. 72(11)

      Pages: 6559-6665

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Functional characterization of two Type III secretion systems of Fibrin parahaemolvticus.2004

    • Author(s)
      K.S.Park, T.Ono, M.Rokuda, M.H.Jang, K.Okada, T.Iida, T.Honda
    • Journal Title

      Infect. Immun. 72(11)

      Pages: 6559-6665

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Genome sequence of Vibrio parahaemolyticus : a pathogenic mechanism distinct from that of V. cholerae2003

    • Author(s)
      Makino K., K.Oshima, K.kurokawa, K.Yokoyama, T.Uda, K.Tagomari, Y.Iijima, M.Najima, M.Nakano, A.Yamashita, Y.Kubota, S.Kimura, T.Yasunaga, T.Honda, H.Shinagawa, M.Hattori, T.Iida
    • Journal Title

      Lancet 361

      Pages: 743-749

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Genome sequence of Vibrio parahaemolyticus : a pathogenic mechanism distinct from that of V cholerae.2003

    • Author(s)
      Makino K., K.Oshima, K.kurokawa, K.Yokoyama, T.Uda, K.Tagomori, Y.Iijima, M.Najima, M.Nakano, A.Yamashita, Y.Kubota, S.Kimura, T.Yasunaga, T.Honda, H.Shinagawa, M.Hattori, T.Iida
    • Journal Title

      Lancet 361

      Pages: 743-749

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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