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2005 Fiscal Year Final Research Report Summary

Molecular population genetic study on the evolution of antigen polymorphism of malaria parasites

Research Project

Project/Area Number 14021125
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionOsaka Institute of Technology

Principal Investigator

TANABE Kazuyuki  Osaka Institute of Technology, Laboratory of Biology, Professor, 工学部, 教授 (40047410)

Co-Investigator(Kenkyū-buntansha) HATTORI Tetsuya  Osaka Institute of Technology, Department of Mathematics, Lecturer, 工学部, 講師 (00288755)
MITA Lbshihiro  Tokyo Women's Medical School, Department of Parasitology, Lecturer, 医学部, 講師 (80318013)
KAWAI Satoru  Dokkyo Medical School, Department of Parasitology, Associate Professor, 医学部, 助教授 (70275733)
Project Period (FY) 2002 – 2005
KeywordsMalaria / Antigen / Polymorphism / Population Genetics / SNP / Tanzania / Plasmodium falcivarum / MSD 1
Research Abstract

The merozoite surface protein 1 (MSP-1) of Plasmodium falciparum is a strong malaria vaccine candidate. However, the highly polymorphic nature of the gene encoding MSP-1 (mspl) presents a potential obstacle to the development of effective vaccines. Also, there is a growing concern of the potential emergence of vaccine resistance because antigen polymorphism is believed to evolve rapidly as seen in resistance to anti-malarial drugs. To assess how rapidly parasites are able to generate novel mspl diversity, investigations of the evolutionary history of mspl polymorphism in modern parasite populations are crucial. Our previous analyses of P falciparum populations from Vanuatu in the southwestern Pacific have shown that single nucleotide polymorphisms (SNPs) in major surface antigen genes, including mspl do not evolve rapidly, whereas repeat length polymorphisms do evolve rapidly. To infer the age of SNPs of mspl in the world parasite populations, we obtained and compared more than 300 complete sequences from geographically diverse areas ; Tanzania, Thailand, Solomon Islands and Brazil. A Tanzanian parasite population showed higher nucleotide diversity than other populations, and a large number of identical SNPs, including synonymous SNPs, were shared between Tanzania and other areas. In contrast to mspl, serca, a selectively neutral gene encoding a P-type calcium-transporting ATPase (PfATP6), had limited numbers of SNPs, as expected, and nucleotide diversity was lower in serca than in mspl in all the geographic areas. Importantly, sharing of identical SNPs was substantially limited in serca between Tanzania and other areas. Together with data of the time to the most recent common ancestor (TMRCA) of parasite populations in geographic areas, our results indicate that much of SNPs in mspl are stable for a considerably long term with the age predating the recent expansion of modern humans out-of-Africa.

  • Research Products

    (12 results)

All 2004 2003 2002

All Journal Article (12 results)

  • [Journal Article] Genetic distance in housekeeping genes between Plasmodium falciparum and Plasmodium reichenowi and within P. falciparum2004

    • Author(s)
      Tanabe, K. et al.
    • Journal Title

      Journal of Molecular Evolution 59

      Pages: 687-694

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Stable SNPs in malaria antigen genes in isolated populations.2004

    • Author(s)
      Tanabe, K. et al.
    • Journal Title

      Science 303

      Pages: 493-

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expansion of wild type allele rather than back mutation in pfcrt explains the recent recovery of chloroquine sensitivity of Plasmodium falciparum in Malawi2004

    • Author(s)
      Mita, T., Tanabe, K.et al.
    • Journal Title

      Molecular and Biochemical Parasitology 135

      Pages: 159-163

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Genetic distance in housekeeping genes between Plasmodium falciparum and Plasmodium reichenowi and within P. falciparum.2004

    • Author(s)
      Tanabe K., Sakihama N., Hattori T., Ranford-Cartwright L., Goldman I., Escalante A.A., Lal A.A.
    • Journal Title

      J. MoL Evol. 59

      Pages: 687-694

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expansion of wild type allele rather than back mutation in pfcrt explains the recent recovery of chloroquine sensitivity of Plasmodium falciparum in Malawi.2004

    • Author(s)
      Mita T., Kaneko A., Lum J.K., Zungu I.L., Tsukahara T., Eto H., Kobayakawa T., Bjorkman A., Tanabe K.
    • Journal Title

      MoL Biochem. ParasitoL 135

      Pages: 159-163

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Stable SNPs in malaria antigen genes in isolated populations.2004

    • Author(s)
      Tanabe K., Sakihama N., Kaneko A.
    • Journal Title

      Science 303

      Pages: 493

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Recovery of chloroquine sensitivity and low prevalence of the Plasmodium falciparum chloroquine resistance transporter gene mutation K76T following the discontinuance of chloroquine use in Malawi.2003

    • Author(s)
      Mita, T., Tanabe, K.et al.
    • Journal Title

      American Journal of Tropical Medicine and Hygiens 68

      Pages: 413-415

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Recovery of chloroquine sensitivity and low prevalence of the Plasmodium falciparum chloroquine resistance transporter gene mutation K76T following the discontinuance of chloroquine use in Malawi.2003

    • Author(s)
      Mita T., Kaneko A., Lum J.K., Bwijo B., Takechi M., Zungu I.L., Tsukahara T., Tanabe K., Kobayakawa T., Bjorkman A.
    • Journal Title

      Am. J. Trop. Med. Hyg. 68

      Pages: 413-415

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] In vitro recombination during PCR of Plasmodium falciparum DNA.2003

    • Author(s)
      Tanabe K., Sakihama N., Farnert A., Rooth I., Bjorkam A., Walliker D., Ranford-Cartwright L.
    • Journal Title

      MoL Biochem. ParasitoL 122

      Pages: 211-216

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] In vitro recombination during PCR of Plasmodium falciparum DNA.2002

    • Author(s)
      Tanabe, K. et al.
    • Journal Title

      Molecular and Biochemical Parasitology 122

      Pages: 211-216

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mosaic organization and heterogeneity in frequency of allelic recombination of the Plasmodium vivax merozoite surface prote in-1 locus.2002

    • Author(s)
      Putaporntip, C., Tanabe, K.et al.
    • Journal Title

      Proceedings of the National Acacemy of Science, USA 99

      Pages: 16348-16353

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mosaic organization and heterogeneity in frequency of allelic recombination of the Plasmodium vivax merozoite surface prote in-1 locus.2002

    • Author(s)
      Putaporntip C., Jongwutiwes S., Sakihama N., Ferreira MAJ., Kho W.-G., Kaneko A., Kanbara H., Hattori T., Tanabe K.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 99

      Pages: 16348-16353

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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