2006 Fiscal Year Final Research Report Summary
Analysis of highly organized functions of the ribosome
| Project/Area Number |
14035103
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | University of Miyazaki (2004-2006)
宮崎医科大学 (2002-2003) |
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Principal Investigator |
KENMOCHI Naoya University of Miyazaki, Frontier Science Resarch Center, Associate Professor (00133124)
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| Project Period (FY) |
2002 – 2006
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| Keywords | ribosome / ribosomapathy / ribosomal protein / disease gene / RPG detabase / snoRNA / zebrafish / Diamond-Blackfan anemia |
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| Research Abstract |
The ribosome is an essential and complex macromolecule responsible for protein synthesis in all organisms. In this study, we have carried out a systematic analysis of ribosomal protein (RP) genes and small nucleolar RNA (snoRNA) genes, and developed animal models to investigate the relationship between the ribosomal abnormalities and human diseases.
1. We have constructed an RP gene database (RPG, http://ribosome.med.miyazaki-u.ac.jp). RPG is highly accessed (>2,000 hits everyday) and has become a standard database for RP gene analysis.
2. We also constructed a snoRNA Orthological database (snoOPY, http://snoopy.med.miyazaki-u.ac.jp/), which provides a powerful tool for studying the ribosomal RNA modifications.
3. We have determined the promoter structures and expression profiles of RP genes in humans and mouse, which allowed us to identify the transcription factors involved in co-regulated RP gene expression, for a better understanding of the molecular mechanism of ribosome synthesis.
4. We have developed zebrafish models with defects in RP genes by inhibiting the mRNA translation using morpholino antisense oligos. The snoRNA expression was also inhibited in the same way. Analyses of these embryos suggested that there is a novel ribosome-dependent regulatory mechanism of gene expression, which should be the key to understanding the 'ribosomapathy'.
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[Journal Article] Ribosomal protein gene knockdown causes developmental defects in zebrafish.2006
Author(s)
Uechi, T., Nakajima, Y., Nakao, A., Torihara, H., Chakraborty, A., Inoue, K. Kenmochi. N.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The human ribosomal protein genes: Sequencing and comparative analysis of 73 genes.2002
Author(s)
Yoshihama, M., Uechi, T., Asakawa, S., Kawasaki, K., Kato, S., Higa, S., Maeda, N., Minoshima, S., Tanaka, T., Shimizu, N. Kenmochi, N.
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Journal Title
Genome Res. 12
Pages: 379-390
Description
「研究成果報告書概要(欧文)」より
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[Presentation] The ribosomal protein genes and Minute loci of Drosophila melanogaster Identification, genetic analysis and determination of gene structure.2006
Author(s)
Mary gold, S. J., Lambertsson, A., Roote, J., East, P., Ashbumer, M., Millburn, G., Harrison, P., Yu, Z., Kenmochi. N,. Leevers, S. J., Kaufman, T. C. Cook, K. R.
Organizer
The 7th International Conference on Ribosome Synthesis
Place of Presentation
Warrenton, USA
Year and Date
20060000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Prediction of snoRNAs in human genome2003
Author(s)
Sagara, J., Nakamura, S., Kenmochi. N., Asai, K.
Organizer
International Conference on "Intelligent Systems for Molecular Biology"
Place of Presentation
Brisbane, Australia
Year and Date
20030000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Prediction of sno RNAs in human genome2002
Author(s)
Sagara, J., Nakamura, S., Kenmochi, N., Sato, T., Okouchi, I., Suwa, M. Asai, K.
Organizer
International Conference on "Intelligent Systems for Molecular Biology"
Place of Presentation
Edmonton, Canada
Year and Date
20020000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Kenmochi. N. The human ribosomal protein genes : Sequencing and comparative analysis2002
Author(s)
Yoshihama, M., Uechi, T., Asakawa, S., Kawasaki, K., Kato, S., Higa, S., Maeda, N., Minosihima, S., Tanaka, T., Shimizu, N.
Organizer
International Conference on "The Dynamics of Ribosome Structure and Function"
Place of Presentation
Queenstown, New Zealand
Year and Date
20020000
Description
「研究成果報告書概要(欧文)」より
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