• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2006 Fiscal Year Final Research Report Summary

Mechanism of tmRNA-mediated trans-translation

Research Project

Project/Area Number 14035201
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNagoya University (2005-2006)
Hirosaki University (2002-2004)

Principal Investigator

AIBA Hiroji  Nagoya University, Graduate School of Science, Professor (20025662)

Co-Investigator(Kenkyū-buntansha) HIMENO Hyouta  Hirosaki University, Biochemistry and Biotechnology, Professor (80208785)
Project Period (FY) 2002 – 2006
KeywordstmRNA / trans-translation / mRNA cleavage / nonstop mRNAs / ribosome / translation surveillance / ribosome stalling / rare codon
Research Abstract

1) Ribosome stalling by the rare codon induces mRNA cleavage, resulting in nonstop mRNAs that are recognized by tmRNA.
2) Ribosome stalling at the cognate stop codon caused by reduced activity or expression of RF-1 or RF-2 induces mRNA cleavage, resulting in nonstop mRNAs that are recognized by tmRNA.
3) Ribosome stalling caused by amino acid starvation induces mRNA cleavage at the corresponding codons, resulting in nonstop mRNAs that are recognized by tmRNA.
4)The ribosome-mediated mRNA cleavage along with the tmRNA system is an important mechanism that enables cells to adapt to amino acid starvation conditions.
5) Trans-translation occurs at the A-site of the ribosome without cleavage of mRNA, and that compeptition among tRNA, tmRNARF and RRF occurs at the A-site.
6) SmpB binds not only the tRNA domain but also the mRNA domain of tmRNA to determine the initiation point of tag-translation.
7) In vitro trans-translation system was developed using purified translation factors. Using this system, we revealed that trans-translation does not require S 1 protein.
8) We have proposed new concepts, "a protein mimicking tRNA+mRNA" and "a protein encoding an amino acid".

  • Research Products

    (41 results)

All 2008 2007 2006 2005 Other

All Journal Article (36 results) (of which Peer Reviewed: 15 results) Book (5 results)

  • [Journal Article] Cleavage of mRNAs and role of tmRNA system under amino acid starvationin Escherichia coli.2008

    • Author(s)
      Li, X., Yagi, M, T., Morita, T., Aiba, H.
    • Journal Title

      Mol. Microbiol 68

      Pages: 462-473

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Reduced action of polypeptide release factors induces mRNA cleavage and tmRNA tagging at stop codons in Escherichia coli.2007

    • Author(s)
      Li, X., Teruaki Yokota, T., Ito, K., Nakamura, Y., Aiba, H.
    • Journal Title

      Mol. Microbiol 63

      Pages: 116-126

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Mechanism of RNA silencing by Hfq・binding small RNAs.2007

    • Author(s)
      Aiba, H.
    • Journal Title

      Curr. Opin. Microbiol. 10

      Pages: 134-139

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Small RNAs making a small protein.2007

    • Author(s)
      T., Morita, T., Aiba, H.
    • Journal Title

      PNAS 104

      Pages: 20149-20150

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] 大腸菌低分子RNAによる遺伝子発現抑制機構2007

    • Author(s)
      森田鉄兵, 饗場弘二
    • Journal Title

      化学と生物 45

      Pages: 298-300

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] In vitro trans・translation of Thermus th erm opbilus : Ribosomal protein Sl is not required for the early stage of trans-translation.2007

    • Author(s)
      Takada, K., Takemoto, C, Kawazoe, M., Konno, T., Hanawa-Suetsugu, K., Lee, S., Shirouzu, M., Yokoyama, S., Muto, A., Himeno, H.
    • Journal Title

      RNA 13

      Pages: 503-510

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] トランス・トランスレーションの分子メカニズム2007

    • Author(s)
      姫野俵太, 栗田大輔, 高田一馬, 今野貴之, 塙(末次)京子, 竹本千重, 川添将仁, 横山茂之, 行木信一, 河合剛太, 武藤あきら
    • Journal Title

      生化学 79

      Pages: 213-221

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Reduced action of polypeptide release factors induces mRNA cleavage and tmRNA tagging at stop codons in Escherichia coli.2007

    • Author(s)
      Li, X., Yokota, T., Ito, K., Nakamura, Y., Aiba, H.
    • Journal Title

      Mol. Microbiol. 63

      Pages: 116-126

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mechanism of RNA silencing by Hfq-binding small RNAs.2007

    • Author(s)
      Aiba, H.
    • Journal Title

      Curr. Opin. Microbiol. 10

      Pages: 134-139

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] In vitro trans-translation of Thermus thermophilus : Ribosomal protein S1 is not required for the early stage of trans-translation.2007

    • Author(s)
      Takada, K., Takemoto, C, Kawazoe, M., Konno, T., Hanawa-Suetsugu, K., Lee, S., Shirouzu, M., Yokoyama, S., Muto, A. & Himeno, H.
    • Journal Title

      RNA 13

      Pages: 503-510

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Protein tagging at rare codons is caused by. tmRNA action at the 3' end ofnonstop mRNA generated in response to ribosome stalling.2006

    • Author(s)
      Li, X., Hirano, R., Tagami, H., Aiba, H.
    • Journal Title

      RNA 12

      Pages: 248-255

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Translational repression is sufficient for gene silencing by bacterial small non-coding RNAs in the absence of mRNA destruction.2006

    • Author(s)
      Morita, T., Mochizuki, Y., Aiba, H.
    • Journal Title

      PNAS 103

      Pages: 1858-4863

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Yeel, a novel protein involved in modulation of the activity of the glucose-phosphotransferase system in Escherichia coli K-12.2006

    • Author(s)
      Becker. A. K., Zeppenfeld, T., Staab, A., Seitz, S., Boos, W., Morita T., Aiba H., Mahr, K., Titgemeyer, F., Jahreis, K.
    • Journal Title

      J Bacteriol 188

      Pages: 5439-5449

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq.2006

    • Author(s)
      Kawamoto, H., Koide, Y., Morita, T., Aiba, H.
    • Journal Title

      Mol. Microbiol. 61

      Pages: 1013-1022

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] 大腸菌small RNAによるRNAサイレンジングの分子機構2006

    • Author(s)
      森田鉄兵, 饗場弘二
    • Journal Title

      蛋白質核酸酵素 : 2006 51

      Pages: 2478-2483

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] An RNA polymerase inhibitor, cyclothiazomycin B 1, and its isomer.2006

    • Author(s)
      Hashimoto, M., Murakami, T., Funahashi, K., Tokunaga, T., Nihei, K., Okuno, T., Kimura, T., Himeno, H.
    • Journal Title

      Bioorg. Med. Chem. 14

      Pages: 8259-8270

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] トランストランスレーション2006

    • Author(s)
      姫野俵太, 武藤あきら
    • Journal Title

      蛋白質核酸酵素 51

      Pages: 2321-2327

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Protein tagging at rare codons is caused by tmRNA action at the 3' end of nonstop mRNA generated in response to ribosome stalling.2006

    • Author(s)
      Li, X., Hirano, R., Tagami, H., Aiba H.
    • Journal Title

      RNA 12

      Pages: 248-255

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Translational repression is sufficient for gene silencing by bacterial small noncoding RNAs in the absence of mRNA destruction.2006

    • Author(s)
      Morita, T., Mochizuki, Y., Aiba H.
    • Journal Title

      PNAS 103

      Pages: 4858-486

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Yeel, a novel protein involved in modulation of the activity of the glucose-phosphotransferase system in Escherichia coli K-12.2006

    • Author(s)
      Becker. A. K., Zeppenfeld, T., Staab, A., Seitz, S., Boos, W., Morita T., Aiba H., Mahr, K., Titgemeyer, R, Jahreis, K.
    • Journal Title

      J Bacteriol 188

      Pages: 5439-5449

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq2006

    • Author(s)
      Kawamoto, H., Koide, Y., Morita, T., Aiba, H.
    • Journal Title

      MoL. Microbiol. 61

      Pages: 1013-1022

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] An RNA polymerase inhibitor, cyclothiazomycin B1, and its isomer.2006

    • Author(s)
      Hashimoto, M., Murakami, T., Funahashi, K, Tokunaga, T., Nihei, K., Okuno, T., Kimura, T. & Himeno, H.
    • Journal Title

      Bioorg. Med. Chem. 14

      Pages: 8259-8270

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Implication of membrane localization of mRNA in the action of a small RNA : mechanism of post-transcriptional regulation of glucose transporter in Escherichia coli.2005

    • Author(s)
      Kawamoto, H., Morita, T., Shimizu, A., Inada, T., Aiba, H.
    • Journal Title

      Genes Dev. 19

      Pages: 328-338

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Translation of aberrant mRNAs lacking a termination codon or with ashortened 3'-UTR is repressed after iniation in yeast.2005

    • Author(s)
      Inada, T., Aiba, H.
    • Journal Title

      EMBO J. 24

      Pages: 1584-1595

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] RNase E-based ribonucleoprotein complexes : mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs.2005

    • Author(s)
      Morita, T., Maki, K., Aiba, H.
    • Journal Title

      Genes Dev. 19

      Pages: 2176-2186

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] グルコース応答と代謝制御ネットワークの新しい世界2005

    • Author(s)
      森田鉄兵, 饗場弘二
    • Journal Title

      化学と生物 43

      Pages: 222-228

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Functional analysis of factors involved in trans・translation.2005

    • Author(s)
      Takada, K., Takemoto, C., Kawazoe, M., Konno, T., Matsuta, N., Kurita, D., Shirouzu, M., Yokoyama, S., Muto, A. & Himeno, H
    • Journal Title

      Nucleic Acids Symp. Ser. 49

      Pages: 101-102

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Interaction analysis between tmRNA and SmpB from Thermus therm ophilus.2005

    • Author(s)
      Nameki, N., Someya, T., Okano, S., Suemasa, R., Kimoto, M., Hanawa-Suetsugu, K., Terada, T., Shirouzu, M., Hirao, I., Takaku, H., Himeno, H., Muto, A., Kuramitsu, S., Yokoyama, S., Kawai, G.
    • Journal Title

      J. Biochem. 138

      Pages: 729-739

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Competition between trans-translation and termination or elongation oftranslation.2005

    • Author(s)
      Asano, K., Kurita, D., Takada, K., Konno, T., Muto, A., Himeno, H.
    • Journal Title

      Nucleic Acids Res. 33

      Pages: 5544-5552

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Implication of membrane localization of target mRNA in the action of a small RNA : mechanism of post-transcriptional regulation of glucose transporter in Escherichia coli.2005

    • Author(s)
      Kawamoto, H., Morita, T., Shimizu, A., Inada, T., Aiba, H.
    • Journal Title

      Genes Dev. 19

      Pages: 328-338

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Translation of aberrant mRNAs lacking a termination codon or with a shortened 3'-UTR is repressed after initiation in yeast.2005

    • Author(s)
      Inada, T., Aiba, T.
    • Journal Title

      EMBO J. 24

      Pages: 1584-1595

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] RNase E-based ribonucleoprotein complexes : mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs .2005

    • Author(s)
      Morita, T., Maki, K., Aiba H.
    • Journal Title

      Genes Dev. 19

      Pages: 2176-2186

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Functional analysis of factors involved in trans-translation.2005

    • Author(s)
      Takada, K, Takemoto, C, Kawazoe, M., Konno, T., Matsuta, N., Kurita, D., Shirouzu, M., Yokoyama, S., Muto, A. & Himeno, H.
    • Journal Title

      Nucleic Acids Symp. Ser. 49

      Pages: 101-102

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Interaction analysis between tmRNA and SmpB from Thermus thermophilus.2005

    • Author(s)
      Nameki, N., Someya, T., Okano, S., Suemasa, R., Kimoto, M., Hanawa-Suetsugu, K., Terada, T., Shirouzu, M., Hirao, I., Takaku, H., Himeno, H., Muto, A., Kuramitsu, S., Yokoyama, S. & Kawai, G.
    • Journal Title

      J. Biochem. 138

      Pages: 729-739

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Competition between trans-translation and termination or elongation of translation.2005

    • Author(s)
      Asano, K, Kurita, D., Takada, K., Konno, T., Muto, A. & Himeno, H.
    • Journal Title

      Nucleic Acids Res. 33

      Pages: 5544-5552

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cleavage of mRNAs and role of tmRNA system under amino acid starvation in Escherichia coli.

    • Author(s)
      Li, X., Yagi, M, T., Morita, T., Aiba, H.
    • Journal Title

      Mol. Microbiol 68

      Pages: 462-473

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] グルコース効果 生化学辞典2007

    • Author(s)
      饗場弘二
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] リボスイッチ 分子細胞生物学辞典2007

    • Author(s)
      饗場弘二
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 転写終結因子の分子機構 バイオインフォーマティクス事典2006

    • Author(s)
      饗場弘二
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 細胞における蛋白質の一生一生成・成熟・輸送・管理・分解・病態2005

    • Author(s)
      姫野俵太, 武藤あきら
    • Total Pages
      5
    • Publisher
      共立出版
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] タンパク質科学イラストレイテッド2005

    • Author(s)
      姫野俵太
    • Total Pages
      11
    • Publisher
      羊土社
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2010-06-09  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi