2006 Fiscal Year Final Research Report Summary
Mechanism of tmRNA-mediated trans-translation
| Project/Area Number |
14035201
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Nagoya University (2005-2006)
Hirosaki University (2002-2004) |
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Principal Investigator |
AIBA Hiroji Nagoya University, Graduate School of Science, Professor (20025662)
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| Co-Investigator(Kenkyū-buntansha) |
HIMENO Hyouta Hirosaki University, Biochemistry and Biotechnology, Professor (80208785)
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| Project Period (FY) |
2002 – 2006
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| Keywords | tmRNA / trans-translation / mRNA cleavage / nonstop mRNAs / ribosome / translation surveillance / ribosome stalling / rare codon |
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| Research Abstract |
1) Ribosome stalling by the rare codon induces mRNA cleavage, resulting in nonstop mRNAs that are recognized by tmRNA.
2) Ribosome stalling at the cognate stop codon caused by reduced activity or expression of RF-1 or RF-2 induces mRNA cleavage, resulting in nonstop mRNAs that are recognized by tmRNA.
3) Ribosome stalling caused by amino acid starvation induces mRNA cleavage at the corresponding codons, resulting in nonstop mRNAs that are recognized by tmRNA.
4)The ribosome-mediated mRNA cleavage along with the tmRNA system is an important mechanism that enables cells to adapt to amino acid starvation conditions.
5) Trans-translation occurs at the A-site of the ribosome without cleavage of mRNA, and that compeptition among tRNA, tmRNARF and RRF occurs at the A-site.
6) SmpB binds not only the tRNA domain but also the mRNA domain of tmRNA to determine the initiation point of tag-translation.
7) In vitro trans-translation system was developed using purified translation factors. Using this system, we revealed that trans-translation does not require S 1 protein.
8) We have proposed new concepts, "a protein mimicking tRNA+mRNA" and "a protein encoding an amino acid".
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[Journal Article] In vitro trans・translation of Thermus th erm opbilus : Ribosomal protein Sl is not required for the early stage of trans-translation.2007
Author(s)
Takada, K., Takemoto, C, Kawazoe, M., Konno, T., Hanawa-Suetsugu, K., Lee, S., Shirouzu, M., Yokoyama, S., Muto, A., Himeno, H.
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Journal Title
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] トランス・トランスレーションの分子メカニズム2007
Author(s)
姫野俵太, 栗田大輔, 高田一馬, 今野貴之, 塙(末次)京子, 竹本千重, 川添将仁, 横山茂之, 行木信一, 河合剛太, 武藤あきら
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Journal Title
Description
「研究成果報告書概要(和文)」より
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[Journal Article] In vitro trans-translation of Thermus thermophilus : Ribosomal protein S1 is not required for the early stage of trans-translation.2007
Author(s)
Takada, K., Takemoto, C, Kawazoe, M., Konno, T., Hanawa-Suetsugu, K., Lee, S., Shirouzu, M., Yokoyama, S., Muto, A. & Himeno, H.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Yeel, a novel protein involved in modulation of the activity of the glucose-phosphotransferase system in Escherichia coli K-12.2006
Author(s)
Becker. A. K., Zeppenfeld, T., Staab, A., Seitz, S., Boos, W., Morita T., Aiba H., Mahr, K., Titgemeyer, F., Jahreis, K.
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Journal Title
J Bacteriol 188
Pages: 5439-5449
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] An RNA polymerase inhibitor, cyclothiazomycin B 1, and its isomer.2006
Author(s)
Hashimoto, M., Murakami, T., Funahashi, K., Tokunaga, T., Nihei, K., Okuno, T., Kimura, T., Himeno, H.
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Journal Title
Bioorg. Med. Chem. 14
Pages: 8259-8270
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Yeel, a novel protein involved in modulation of the activity of the glucose-phosphotransferase system in Escherichia coli K-12.2006
Author(s)
Becker. A. K., Zeppenfeld, T., Staab, A., Seitz, S., Boos, W., Morita T., Aiba H., Mahr, K., Titgemeyer, R, Jahreis, K.
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Journal Title
J Bacteriol 188
Pages: 5439-5449
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] An RNA polymerase inhibitor, cyclothiazomycin B1, and its isomer.2006
Author(s)
Hashimoto, M., Murakami, T., Funahashi, K, Tokunaga, T., Nihei, K., Okuno, T., Kimura, T. & Himeno, H.
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Journal Title
Bioorg. Med. Chem. 14
Pages: 8259-8270
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Functional analysis of factors involved in trans・translation.2005
Author(s)
Takada, K., Takemoto, C., Kawazoe, M., Konno, T., Matsuta, N., Kurita, D., Shirouzu, M., Yokoyama, S., Muto, A. & Himeno, H
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Journal Title
Nucleic Acids Symp. Ser. 49
Pages: 101-102
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Interaction analysis between tmRNA and SmpB from Thermus therm ophilus.2005
Author(s)
Nameki, N., Someya, T., Okano, S., Suemasa, R., Kimoto, M., Hanawa-Suetsugu, K., Terada, T., Shirouzu, M., Hirao, I., Takaku, H., Himeno, H., Muto, A., Kuramitsu, S., Yokoyama, S., Kawai, G.
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Journal Title
J. Biochem. 138
Pages: 729-739
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Competition between trans-translation and termination or elongation oftranslation.2005
Author(s)
Asano, K., Kurita, D., Takada, K., Konno, T., Muto, A., Himeno, H.
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Journal Title
Nucleic Acids Res. 33
Pages: 5544-5552
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Functional analysis of factors involved in trans-translation.2005
Author(s)
Takada, K, Takemoto, C, Kawazoe, M., Konno, T., Matsuta, N., Kurita, D., Shirouzu, M., Yokoyama, S., Muto, A. & Himeno, H.
-
Journal Title
Nucleic Acids Symp. Ser. 49
Pages: 101-102
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Interaction analysis between tmRNA and SmpB from Thermus thermophilus.2005
Author(s)
Nameki, N., Someya, T., Okano, S., Suemasa, R., Kimoto, M., Hanawa-Suetsugu, K., Terada, T., Shirouzu, M., Hirao, I., Takaku, H., Himeno, H., Muto, A., Kuramitsu, S., Yokoyama, S. & Kawai, G.
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Journal Title
J. Biochem. 138
Pages: 729-739
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Competition between trans-translation and termination or elongation of translation.2005
Author(s)
Asano, K, Kurita, D., Takada, K., Konno, T., Muto, A. & Himeno, H.
-
Journal Title
Nucleic Acids Res. 33
Pages: 5544-5552
Description
「研究成果報告書概要(欧文)」より
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