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2006 Fiscal Year Final Research Report Summary

Surface Measurement Related on Surface Pasmon Resonance Sensing

Research Project

Project/Area Number 14050088
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionKeio University

Principal Investigator

SUZUKI Koji  Keio University, Faculty of Science and Technology, Professor (80154540)

Co-Investigator(Kenkyū-buntansha) KOMATSU Hirokazu  Keio University, Faculty of Science and Technology, Reserch Associate (10407140)
OSAWA Masatoshi  Hokkaido University, Catalysis Research Center, Professor (00108466)
Project Period (FY) 2001 – 2006
KeywordsSPR sensor / Surface plasmon resonance / Surface imaging / Biosensing / Micro-fiber sensing / Optical waveguide sensor / Polymer nano-perticle / Response amplification
Research Abstract

A surface plasmon resonance (SPR) sensor is a useful analytical device that can measure a surface reaction between an analyte and a sensor chip using a gold thin film. Our research purpose is to develop practically useful SPR sensors for wider applications for the analysis of mostly biological samples. For this purpose, highly sensitive measurements are required that can be realized based on the two methods as listed below.
1) For realizing high sensitivity in SPR sensor response by physical methods, two new types of SPR sensors were developed.
i) Micro-fiber SPR sensor:
A germanium-doped optical silica fiber was chemically attached and a thin gold layer of 50nm thickness was placed on the fiber surface by vapor deposition. This micro-SPR sensor can measure down to one picoliter volume sample with reflective index value changes at the 10-3 order level.
ii) Slab optical waveguide (SOWG) SPR sensor:
Multiple optical reflections are a useful way to reach a highly sensitive detection limit. A5 … More to 10mm SOWG-SPR sensor was made using a glass film as the sensor chip. The sensor chip has a 50nm thin gold layer that can also be used as an electrode for voltammetric measurements. A ferrocene-containing self-assembled monolayer (FC-SAM) was immobilized on the sensor chip surface and the redox reaction was measured by both SPR sensing and voltammetric methods. In this case, real time analysis can be performed to measure redox reactions by both methods. The redox reaction products can be accurately determined with the SPR method.
2) Realizing high sensitivity in SPR sensor response by a chemical method:
Aconventional commercialized SPR sensor is normally measuring the real part of the reflective index (RI) value. On the other hand, we have clarified the usefulness of measuring the imaginary part of the RI value in the SPR measurement. An absorption-based SPR sensor was prepared relying on the theoretical understanding of the methodology based on imaginary part RI measurements. Consequently, the method was applied to determine low molecular weight substances, such as glucose, which are normally difficult to detect with a conventional SPR sensor. Furthermore, a polymer nanoparticle, which can amplify the SPR response, was prepared as a chemical amplification labeling reagent for a SPR sensor, that is useful for immuno-sensing with high sensitivity Less

  • Research Products

    (8 results)

All 2006 2005 2004 2003 2002

All Journal Article (5 results) (of which Peer Reviewed: 3 results) Presentation (2 results) Book (1 results)

  • [Journal Article] SPR sensor signal amplification based on dye-doped polymer particles2006

    • Author(s)
      H. Komatsu, K. Suzuki, et al.
    • Journal Title

      SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS 7・2

      Pages: 150-155

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] SPR sensor signal amplification based on dye-doped polymer particles2006

    • Author(s)
      H. Komatsu, K. Suzuki, et al.
    • Journal Title

      Science and Technology of Advanced Materials 7(2)

      Pages: 150-155

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Fiber-optic conical microsensors for surface plasmon resonance using chemically etched single-mode fiber2004

    • Author(s)
      K. Kurihara, K. Suzuki, et al.
    • Journal Title

      Analytica Chimica Acta 534

      Pages: 165-170

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Fiber-optic conical microsensors for surface plasmon resonance using chemically etched single-mode fiber2004

    • Author(s)
      K. Kurihara, K. Suzuki
    • Journal Title

      Analytica Chimica Acta 534

      Pages: 165-170

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Application of Absorption-Based Surface Plasmon Resonance Principle to the Development of SPR Ammonium Ion and Enzyme Sensors2002

    • Author(s)
      E. Fujii, K. Suzuki, et al.
    • Journal Title

      Analytical Chemistry 74

      Pages: 6106-6110

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Presentation] 光を用いる界面化学計測の展開2005

    • Author(s)
      鈴木孝治
    • Organizer
      第85回春季年会特別企画講演
    • Place of Presentation
      横浜
    • Year and Date
      2005-03-26
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Surface measurements using optical methods2005

    • Author(s)
      Koji Suzuki
    • Organizer
      The 85^<th> CSJ annual meeting
    • Place of Presentation
      Yokohama, JAPAN
    • Year and Date
      2005-03-26
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 機器分析化学 10. 分子間力を用いる分析法2003

    • Author(s)
      栗原一嘉, 鈴木孝治
    • Total Pages
      8
    • Publisher
      朝倉書店
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-06-09  

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