2006 Fiscal Year Final Research Report Summary
Functional analysis of scaffold proteins for mammalian stress-responsive MAP kinase signaling pathways
| Project/Area Number |
14086205
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Kanazawa University |
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Principal Investigator |
YOSHIOKA Katsuji Kanazawa University, Cancer Research Institute, Professor (60200937)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Michihiko Kitasato University, School of Science, Associate Professor (90240994)
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| Project Period (FY) |
2002 – 2006
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| Keywords | cadherin / cerebellum / deveropment and differentiation / genetically engineered mice / JNK / MAP kinase / scaffold protein / signal transduction |
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| Research Abstract |
Scaffold proteins of the mammalian MAP kinase (MAPK) cascades are considered having critical roles in spatio-temporal regulation of MAPK pathways by organizing their signaling components into functional modules. We are particularly interested in the functions of these scaffold proteins, mainly c-Jun NH_2-terminal kinase (JNK)/stress-activated protein kinase-associated protein 1 (JSAP1); a scaffold protein that participates in JNK MAPK cascades, both in vitro and in vivo. Our findings are summarized as follows :
1) We first investigated the JSAP1-null ES cells. We found that the cardiomyogenesis and neurogenesis process in JSAP1-null mutants were seriously impaired, which strongly indicated that JSAP1 plays an important role in cardiomyocyte and neural development (JBC, 2002 ; BBRC, 2005).
2) We also demonstrated that JSAP1 regulates cell movement in cooperation with the focal adhesion kinase (Oncogene, 2002 ; JBC 2005).
3) We further showed that JSAP1 scaffold regulates cell-cell interact
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ions in PC12h cells specifically in the NGF-induced signaling pathway, and does so by modulating N-cadherin (BBRC, 2007) through the knock down experiments in PC12h cells.
4) We also studied JSAP1 and JNK expression in mouse brains. Our results obtained by in situ hybridization and immunohistochemical analyses strongly suggested that JSAP1-JNK signaling plays important roles in developing and adult mouse brains (JNC, 2006).
5) During the development of the cerebellum, massive clonal expansion of granule cell precursors (GCPs) occurs in the outer part of the external granular layer (EGL). We have provided evidence that JSAP1 and active JNK were expressed preferentially in the post-mitotic inner EGL progenitors in the developing cerebellum. Moreover, jsapl deficiency resulted in increasing numbers of proliferating GCPs in mouse embryos. Besides, overexpression of JSAP1 in cultured GCPs led to increased numbers of NeuN-positive cells together with the activation of JNK. Together, these data strongly indicated that JSAP1 promotes the cell-cycle exit and differentiation of GCPs by modulating JNK activity in cerebellar development (in preparation). Less
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Research Products
(40 results)
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[Journal Article] Regulation of N-cadherin-based cell-cell interaction by JSAP1 scaffoldinPC12h cells.2007
Author(s)
Bayarsaikhan, M., Takino, T., Gantulga, D., Sato, H., Ito, T., ^*Yoshioka, K.
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Journal Title
Biochem. Biophys. Res. Commun. 353
Pages: 357-362
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] 2-methoxyestradiol, an endogenous mammalian metabolite, radiosensitizes colon carcinoma cells through c-Jun NH2-terminal kinase activation.2006
Author(s)
Zou, H. C. Adachi, M., Imai, K., Hareyama, M., Yoshioka, K., Zhao, S., ^*Shinomura, Y.
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Journal Title
Clin. Cancer Res. 12
Pages: 6532-6539
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] c-jun N-terminal kinase hyperphosphorylates R406W tau at the PHF-1 site during mitosis.2006
Author(s)
Tatebayashi, Y., Planel, E., Chui, D. H., Sato, S., Miyasaka, T., Sahara, N., Murayama, M. Kikuchi, N., Yoshioka, K., Rivka, R., ^*Takashima, A.
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Journal Title
FASEB J. 20
Pages: 752-764
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Expression and distribution of JNK/SAPK-associated scaffold protein JSAP1 in developing and adult mouse brain.2006
Author(s)
Miura, E., Fukaya, M., Sato, T., Sugihara, K., Asano M, Yoshioka, K., ^*Watanabe M.
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Journal Title
J. Neurochem. 97
Pages: 1431-1436
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification and characterization of mouse PSF1-binding protein, SLD5.2006
Author(s)
Kong, L., Ueno, M., Itoh, M., Yoshioka, K., ^*Takakura, N.
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Journal Title
Biochem. Biophys. Res. Commun. 339
Pages: 1204-1207
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Convergence of cell cycle regulation and growth factor signals on GRASP65.2005
Author(s)
Yoshimura, S., Yoshioka, K., Barr, F. A., Lowe, M., Nakayama, K., Ohkuma, S., ^*Nakamura, N.
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Journal Title
J. Biol. Chem. 280
Pages: 23048-23056
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] JSAP1/JIP3 cooperates with FAK to regulate c-Jun N-terminal kinase and cell migration.2005
Author(s)
^*Takino, T., Nakada, M., Miyamori, H., Watanabe, Y., Sato, T., Gantulga D., Yoshioka, K., Yamada, K. M., Sato, H.
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Journal Title
J. Biol. Chem. 280
Pages: 37772-37781
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Impairment of cardiomyogenesis in embryonic stem cells lacking scaffold protein JSAP1.2005
Author(s)
Sato, T., Hidaka, K., Iwanaga, A., Ito, M., Asano, M., Nakabeppu, Y., Morisaki, T., ^*Yoshioka, K.
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Journal Title
Biochem. Biophys. Res. Commun. 338
Pages: 1152-1157
Description
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[Journal Article] Activation mechanism of c-Jun amino-terminal kinase in the course of neural differentiation of P19 embryonic carcinoma cells.2004
Author(s)
Akiyama, S., Yonezawa, T., Kudo, T., Li, M. G., Wang, H., Ito, M., Yoshioka, K., Ninomiya-Tsuji, J., Matsumoto, K., Kanamaru, R., ^*Tamura, S., ^*Kobayashi, T.
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Journal Title
J. Biol. Chem. 279
Pages: 36616-36620
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Critical roles of c-Jun signaling in regulation of NFAT family and RANKL-regulated osteoclast differentiation.2004
Author(s)
Ikeda, F., ^*Nishimura, R., Matsubara, T., Tanaka, S., Inoue, J., Reddy, S. V., Hata, K., Yamashita, K., Hiraga, T., Watanabe, T., Kukita, T., Yoshioka, K., Rao, A., Yoneda, T.
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Journal Title
J. Clin. Invest. 114
Pages: 475-484
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Membrane type 1 matrix metalloproteinase regulates collagen-dependent mitogen-activated protein/extracellular signal-regulated kinase activation and cell migration.2004
Author(s)
Takino, T., Miyamori, H., Watanabe, Y., Yoshioka, K., Seiki, M., ^*Sato, H.
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Journal Title
Cancer Res. 64
Pages: 1044-1049
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] JNK promotes Bax translocation to mitochondria through phosphorylation of 14-3-3 proteins.2004
Author(s)
Tsuruta, F., Sunayama, J., Mori, Y., Hattori, S., Shimizu, S., Tsujimoto, Y., Yoshioka, K., Masuyama, N., ^*Gotoh, Y.
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Journal Title
EMBO J. 23
Pages: 1889-1899
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Caspase-mediated cleavage of JNK during stress-induced apoptosis.2003
Author(s)
Enomoto, A., Suzuki, N., Morita, A., Ito, M., Liu, C. Q., Matsumoto, Y., Yoshioka. K., Shiba, T., ^*Hosoi, Y.
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Journal Title
Biochem. Biophys. Res. Commun. 306
Pages: 837-842
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] In vitro development of mouse embryonic stem cells lacking JNK/stress-activated protein kinase-associated protein (JSAP1) scaffold protein revealed its requirement during early embryonic neurogenesis.2003
Author(s)
Xu, P., Yoshioka, K., Yoshimura, D., Tominaga, Y., Nishioka, T., Ito, M., ^*Nakabeppu, Y.
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Journal Title
J. Biol. Chem. 278
Pages: 48422-48433
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Aberrant tau phosphorylation by glycogen synthase kinase-3b and JNK3 induces oligomeric tau fibrils in COS-7 cells.2002
Author(s)
Sato, S., Tatebayashi, Y., Akagi, T., Chui, D. -H., Murayama, M., Miyasaka, T., Planel, E., Tanemura, K., Sun, X., Hashikawa, T., Yoshioka, K., Ishiguro, K., ^*Takashima, A.
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Journal Title
J. Biol. Chem. 277
Pages: 42060-42065
Description
「研究成果報告書概要(欧文)」より
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