2006 Fiscal Year Final Research Report Summary
Sensors of Environmental Stress
| Project/Area Number |
14086207
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | National Institute for Basic Biology (2004)
Okazaki National Research Institutes (2002-2003) |
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Principal Investigator |
MURATA Norio Okazaki National Research Institutes, 基礎生物学研究所, Professor (90011569)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Iwane National Institutes of Natural Sciences, National Institute for Basic Biology, Assistant Professor (10290909)
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| Project Period (FY) |
2002 – 2004
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| Keywords | cyanobacterium / DNA microarray / histiding kinase / hyperosmotic stress / response regulator / salt stress / Synechocystis sp. PCC 6803 / Two-component system |
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| Research Abstract |
Histidine kinase (Hik) and response regulator (Rre) constitute the twp-component system that is a signal perception/transduction pathway in prokaryotes and some eukaryotes. The cyanobacterium Synechocystis sp. PCC 6803, which performs oxygenic photosynthesis like plants, contains 47 genes for Hiks and 45 genes for Rres. We mutated these genes successfully, except three genes for Hiks and three genes for Rres, and constructed mutant libraries of Hiks and Rres. By screening the libraries by genome-wide expression of genes under various stress conditions with cDNA microarrays, we obtained the following results.
Hyperosmotic stress, due to 0.5 M sorbitol, induced the expression of 246 genes. Screening of the above-mentioned mutant libraries with respect to the hyperosmotic stress-inducible expression of genes by the cDNA microarray method revealed that 60% of the hyperosmotic stress-inducible genes are under control of five two-component systems, namely, Hik33/Rre31, Hik16/Hik41/Rre17, Hik34/Rre1, Hik2/Rre1, and Hik10/Rre3. Remaining 40% of the hyperosmotic stress-inducible genes are under control of as-yet unidentified signal-transducing pathways.
Salt stress, due to 0.5 M NaCl, induced the expression of 294 genes. Screening of the above-mentioned mutant libraries with respect to the salt stress-inducible expression of genes by the cDNA microarray method revealed that 70% of the salt stress-inducible genes are under control five two-component systems as in the case of hyperosmotic stress-inducible genes. However, genes under the individual Hik/Rre two-component systems are different between the salt stress and hyperosmotic stress. Remaining 30% of the salt stress-inducible genes are under control of as-yet unidentified signal-transducing pathways.
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[Journal Article] Histidine kinases play important roles in the perception and signal transduction of hydrogen peroxide in the cyanobacterium Synechocystis sp. Pcc 6803.2007
Author(s)
Kanesaki, Y., Yamamoto, H., Paithoonrangsarid, K., Shoumskaya, M., Suzuki. I., Hayashi, H., Murata. N.
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Journal Title
Plant J. 49
Pages: 313-324
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Serine/threonine protein kinase SpkA in Synechocystis sp. strain PCC 6803 is a regulator of expression of three putative pilA operon, formation of thick pili, and cell motility.2006
Author(s)
Panichkin, V. B., Arakawa-Kobayashi, S., Kanaseki, T., Suzuki. I., Los, D. A., Shestakov, S. V., Murata. N.
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Journal Title
J. Bacteriol. 188
Pages: 7696-7699
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The histidine kinase Hik34 is involved in thermotolerance by regulating the expression of heat-shock genes in Synechocystis.2005
Author(s)
Suzuki, I., Kanesaki, Y., Hayashi, H., Hall, J. J., Simon, W. J., Slabas, A. R., Murata. N.
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Journal Title
Plant Physiol. 138
Pages: 1409-1421
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identical Hik-Rre systems are involved in perception and transduction of salt signals and hyperosmotic signals but regulate the expression of individual genes to different extents in Synechocystis.2005
Author(s)
Shoumskaya, M., Paithoonrangsarid, K., Kanesaki, Y., Los, D. A., Zinchenko, V., Tanticharoen, M., Suzuki, I., Murata, N.
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Journal Title
J. Biol. Chem. 280
Pages: 21531-21538
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Gene expression profiling reflects physiological processes in salt acclimation in Synechocystis sp. PCC 6803.2004
Author(s)
Marin, K., Kanesaki, Y., Los, D. A., Murata, N., Suzuki, I., Hagemann, M.
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Journal Title
Plant Physiol. 136
Pages: 3290-3300
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Glucosylglycerol, a compatible solute, sustains cell division under salt stress.2003
Author(s)
Ferjani, A., Mustardy, L., Sulpice, R., Marin, K., Suzuki, I., Hagemann, M., Murata, N.
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Journal Title
Plant Physiol. 131
Pages: 1628-1637
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Gene-engineered rigidification of membrane lipids enhances the cold inducibility of gene expression in Synechosystis.2003
Author(s)
Inaba, M., Suzuki, I., Szalontai, B., Kanesaki, Y., Los, D. A., Hayashi, H., Murata, N.
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Journal Title
J. Biol. Chem. 278
Pages: 12191-12198
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Dissecting a cyanobacterial proteolytic system : efficiency in inducing degradation of the D1 protein of photosystem II in cyanobacteria and plants.2003
Author(s)
Kanervo, E., Spetea, C., Nishiyama, Y., Murata, N., Andersson, B., Aro, E.
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Journal Title
Biochim. Biophys. Acta (Bioenergetics) 1607
Pages: 131-140
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of histidine kinases that act as sensors in the perception of salt stress in Synechocystis sp. PCC 6803.2003
Author(s)
Marin, K., Suzuki, I., Yamaguchi, K., Yammoto, H., Ribbeck, K., Kanesaki, Y., Hagemann, M., Murata, N.
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Journal Title
Proc. Natl. Acad. Sci. USA 100
Pages: 9061-9066
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Sensors of abiotic stress in Synechocystis.2003
Author(s)
Mikami, K., Suzuki, I., Murata, N.
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Journal Title
In Topics in Current Genetics, (Hirt, H., Shinozaki, K., Eds). Springer, Berlin. 4
Pages: 103-119
Description
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[Journal Article] Salt stress inhibits the repair of photodamaged photosystem II by supressing the transcription and translation of psbA genes in Synechosystis.2002
Author(s)
Allakhverdiev, S. I., Nishiyama, Y., Miyairi, S., Yamamoto, H., Inagaki, N., Kanesaki, Y., Murata, N.
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Journal Title
Plant Physiol. 130
Pages: 1443-1453
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Transgenics of an elite indica variety Pusa Basmati 1 harbouring the codA gene are highly to lerant to salt stress.2002
Author(s)
Mohanty, A., Kathuria, H., Ferjani, A., Sakamoto, A., Mohanty, P., Murata, N., Tyagi, A. K.
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Journal Title
Theoret. Appl. Genetics 106
Pages: 51-57
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A two-component Mn^<2+>-sensing system negatively regulates expression of the mntCAB operon in Synechocystis.2002
Author(s)
Yamaguchi, K., Suzuki, I., Yamamoto, H., Lyukevich, A., Bodrova, I., Los, D. A., Piven, I., Zinchenko, V., Kanehisa, M., Murata, N.
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Journal Title
Plant Cell 14
Pages: 2901-2913
Description
「研究成果報告書概要(欧文)」より
