2006 Fiscal Year Final Research Report Summary
Molecular characterization of chloride cells and their mechanism of differentiation
| Project/Area Number |
14104002
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
生物形態・構造
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
HIROSE Shigehisa Tokyo Institute of Technology, Department of Biological Sciences, Professor, 大学院生命理工学研究科, 教授 (10134199)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEI Yoshio University of Tokyo, Ocean Research Institute, Professor, 海洋研究所, 教授 (10129249)
KATO Akira Tokyo Institute of Technology, Department of Biological Sciences, Assistant Professor (40311336)
HOSHIJIMA Kazuyuki Tokyo Institute of Technology, Department of Biological Sciences, Assistant Professor (70397032)
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| Project Period (FY) |
2002 – 2006
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| Keywords | chloride cell / Osorezan dace / mitochondria / acid adaptation / channel / transporter / mitochondria-rich cell / foxi3a |
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| Research Abstract |
We mainly focused on the chloride cells that are very unique in the structure and function and play key roles in adapting to low or high salt conditions and acidic or alkaline conditions. Our analyses contributed to pushing the forefront of not only osmoregulation of aquatic animals but also fluid homeostasis of the mammalian system. Furthermore mitochondrion-rich nature of chloride cells led us to explore the mitochondrial morphology and to discover a novel regulator of mitochondrial fusion and fission factors. (1) We have clarified the molecular mechanism underlying the acid adaptation of Osorezan dace living in a pH 3.5 lake by identifying molecules associated with gross morphological and functional changes of chloride cells. Increased expression of glutamate dehydrogenase was also observed in various tissues of acid-adapted dace suggesting a significant role of ammonia and bicarbonate generated by glutamine catabolism. (2) We succeeded in visualizing mitochondrion-rich cells (MRCs)
… More
responsible for uptake of Na^+ in freshwater using a fluorescent dye, Sodium Green. Combined with immunohistochemistry, we revealed that the Na^+-absorbing MRCs were exceptionally rich in vacuolar-type H^+-ATPase (H^+-ATPase) but moderately rich in Na^+/K^+-ATPase. (3) Through molecular, functional, and immunohistochemical characterization of ammonia transporters in fish for the first time, we demonstrated a tight link of ammonia excretion with MRCs and suggested a functional coupling between ion homeostasis and nitrogen metabolism. (4) We also addressed the function of foxi3a, a transcription factor that is specifically expressed in the H^+-ATPase-rich MR cells. When foxi3a was depleted from zebrafish embryos by antisense morpholino oligonucleotide injection, differentiation of the MRCs was completely blocked and Na+ influx was severely reduced, indicating that MRCs are the primary sites for Na^+ absorption. Considering these results with onset of the expression at gastrula stage, we proposed that foxi3a is a key gene for the control of MRC differentiation. The delta-notch signaling system has also been demonstrated to be involved in the differentiation and distribution of MRCs. (5) Mitochondria frequently fuse and divide to form a dynamic network in various eukaryotic cells. We showed that MARCH5 is a mitochondrial outer membrane protein and plays a crucial role in control of mitochondrial morphology by regulating Mfn2 (a fusion factor) and Drp1 (a fission factor) activities. Less
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[Journal Article] (2007) Visualization in zebrafish larvae of Na^+ uptake in mitochondria-rich cells whose differentiation is dependent on foxi3a2007
Author(s)
Esaki, M., Hoshijima, K., Kobayashi, S., Fukuda, H., Kawakami, K., Hirose, S.
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Journal Title
Am.J.Physiol. 292
Pages: R470-R480
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] (2006) Molecular and functional characterization of adrenomedullin receptors in pufferfish2006
Author(s)
Nag, K., Kato, A., Nakada, T., Hoshijima, K., Mistry, A.C., Takei, Y., Hirose, S.
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Journal Title
Am.J.Physiol. 290
Pages: R467-R478
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] (2006) Spliced isoforms of LIM-domain-binding protein (CLIM/NLI/Ldb) lacking LIM-interaction domain2006
Author(s)
Tran, Y.H., Xu, Z., Kato, A., Mistry, A.C., Goya, Y., Taira, M., Brandt, S.J., Hirose, S.
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Journal Title
J.Biochem. 140
Pages: 105-119
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] (2005) Identification, evolution, and regulation of expression of guinea pig trappin with an unusually long transglutaminase substrate domain2005
Author(s)
Furutani, Y., Kato, A., Fibriani, A., Hirata, T., Kawai, R., Jeon, J.-H., Fujii, Y., Kim, I.-G., Kojima, S., Hirose, S.
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Journal Title
J.Biol.Chem. 280
Pages: 20204-20215
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] (2005) NHE3 in an ancestral vertebrate : primary sequence, distribution, localization, and function in gills2005
Author(s)
Choe, K.P., Kato, A., Hirose, S., Plata, C., Sindic, A., Romero, M.F., Claiborne, J.B., Evans, D.H.
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Journal Title
Am.J.Physiol. 289
Pages: R1520-R1534
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] (2005) Roles of Slc13a1 and Slc26a1 sulfate transporters of eel kidney in sulfate homeostasis and osmoregulation in freshwater2005
Author(s)
Nakada, T., Zandi-Nejad, K., Kurita, Y., Kudo, H., Broumand, V., Kwon, C.Y., Mercado, A., Mount, D.B., Hirose, S.
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Journal Title
Am.J.Physiol. 289
Pages: R575-R585
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] (2005) A novel type of urea transporter, UT-C, highly expressed in proximal tubule of seawater eel kidney2005
Author(s)
Mistry, A.C., Chen, G., Kato, A., Nag, K., Sands, J.M., Hirose, S.
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Journal Title
Am.J.Physiol. 288
Pages: F455-F465
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] (2004) FHL5, a novel actin fiber-binding protein, is highly expressed in gill pillar cells and responds to wall tension in eels2004
Author(s)
Mistry, A.C., Kato, A., Tran, Y., Honda, S., Tsukada, T., Takei, Y., Hirose, S.
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Journal Title
Am.J.Physiol. 287
Pages: R1141-R1154
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] (2003) Mechanism of acid adaptation of a fish living in a pH 3.5 lake2003
Author(s)
Hirata, T., Kaneko, T., Ono, T., Nakazato, T., Furukawa, N., Hasegawa, S., Wakabayashi, S., Shigekawa, M., Chang, M.H., Romero, M., Hirose, S.
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Journal Title
Am.J.Physiol. 284
Pages: R1199-R1212
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] (2003) Expression of the K^+ channel Kir7.1 in the developing rat kidney : role in K^+ excretion2003
Author(s)
Suzuki, Y., Yasuoka, Y., Shimohama, T., Nishikitani, M., Nakamura, N., Hirose, S., Kawahara, K.
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Journal Title
Kidney Int. 63
Pages: 969-975
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] (2003) Four functionally distinct C-type natriuretic peptides found in fish reveal evolutionary history of the natriuretic peptide system2003
Author(s)
Inoue, K., Naruse, K., Yamagami, S., Mitani, H., Suzuki, N., Takei, Y.
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Journal Title
Proc.Natl.Acad.Sci.USA. 100
Pages: 10079-10084
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] (2003) An interacting network of T-box genes directs gene expression and fate in the zebrafish mesoderm2003
Author(s)
Goering, L.M., Hoshiiima, K., Hug, B., Bisgrove, B., Kispert, A., Grunwald, D.J.
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Journal Title
Proc.Natl.Acad.Sci.USA. 100
Pages: 9410-9415
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] (2002) RING finger, B-box, and coiled-coil (RBCC) protein expression in branchial epithelial cells of Japanese eel, Anguilla japonica2002
Author(s)
Miyamoto, K., Nakamura, N., Kashiwagi, M., Honda, S., Kato, A., Hasegawa, S., Takei, Y., Hirose, S.
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Journal Title
Eur.J.Biochem. 269
Pages: 6152-6161
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] (2002) Kidney-specific chloride channel, OmC1C-K, predominantly expressed in the diluting segment of freshwater-adapted tilapia kidney2002
Author(s)
Miyazaki, H., Kaneko, T., Uchida, S., Sasaki, S., Takei, Y.
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Journal Title
Proc.Natl.Acad.Sci.USA. 99
Pages: 15782-15787
Description
「研究成果報告書概要(欧文)」より
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