2005 Fiscal Year Final Research Report Summary
Development of micro in situ analysis devices for microbiology in oceanic environment
| Project/Area Number |
14205147
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
海洋工学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YAMAMOTO Takatoki The University of Tokyo, Institute of Industrial Science, Research associate, 生産技術研究所, 助手 (20322688)
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| Co-Investigator(Kenkyū-buntansha) |
FUJII Teruo The University of Tokyo, Institute of Industrial Science, Associate professor, 生産技術研究所, 助教授 (30251474)
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| Project Period (FY) |
2002 – 2005
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| Keywords | microfluidic device / in situ analysis system / gene analysis / deep-sea / IISA-Gene / PCR / Microbes / PDMS |
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| Research Abstract |
In this research, an in situ analysis device for microbiology is developed and evaluated with microfabrication techniques. As a result, IISA (Integrated In situ Analyzer) -Gene was successfully developed with compact format. To achieve the device, development and evaluation was performed as follows :
(1)A PDMS based microchannel chip and a glass based temperature control chip was fabricated to perform molecular analysis of microbes living in oceanic environments with PCR method.
(2)Surfactant reagent (Tween 20) was applied to prevent protein adsorption onto PDMS surface. It increased reaction performance effectively.
(3)Chaotropic reagent (GuSCN) and glass beads based DNA purification function was integrated to the device.
(4)LED light source and photo detector (PMT) was integrated to the device for optical detection of PCR products.
(5)For in situ operation, miniaturized pumping system with pumps and valves is connected to the microchip.
(6)Microprocessor based controller circuit and power supply are packaged in a pressure resistant housing. IISA-Gene was mounted in oil-filled housing for pressure equalization.
(7)High-pressure experiment system was developed and utilized for evaluations of the IISA-Gene. It can simulate high pressure (up to 60 MPa) and low temperature (down to 2℃).
The IISA-Gene was operated in real deep-sea environment with RV "NATSUSHIMA" and ROV "HYPERDOLPHIN" (JAMSTEC, Japan) at off Ishigaki Isl. Japan. As a result, the system was operated successfully and the primitive data was obtained.
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[Journal Article] 深海熱水地帯における現場複合計測技術の開発-熱水プルームマッピングと現場遺伝子解析の試み2006
Author(s)
藤井輝夫, 福場辰洋, 岡本拓士, 長沼毅, 金子亮, 林徹, 吉田尊雄, 許正憲, 岡村慶, 下島公紀, 前田義明, 小池祐一
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Journal Title
第22回しんかいシンポジウム(Blue Earth'06)予稿集
Pages: 60-61
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Development of in situ multi analysis system -trial for hot plume mapping and in situ gene analysis2006
Author(s)
T.Fujii, T.Fukuba, T.Okamoto, T.Naganuma, R.Kaneko, T.Hayashi, T.Yoshida, M.Kyo, K.Okamura, K.Shitashima, Y.Maeda, Y.Koike
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Journal Title
Blue Earth '06
Pages: 60-61
Description
「研究成果報告書概要(欧文)」より
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