2005 Fiscal Year Final Research Report Summary
Analysis and identification of anther-specific genes and male-sterility genes
| Project/Area Number |
14206001
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Tohoku University |
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Principal Investigator |
NISHIO Takeshi Tohoku University, Graduate School of Agricultural Science, Professor, 大学院・農学研究科, 教授 (30301039)
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| Co-Investigator(Kenkyū-buntansha) |
TORIYAMA Kinya Tohoku University, Graduate School of Agricultural Science, Professor, 大学院・農学研究科, 教授 (20183882)
KISHITANE Sachie Tohoku University, Graduate School of Agricultural Science, Assistant professor, 大学院・農学研究科, 助手 (60005634)
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| Project Period (FY) |
2002 – 2005
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| Keywords | male sterility / anther-specific gene / cytoplasmic male sterility / fertility-restorer gene / nuclear-genic male sterility / mutated gene / Brassicaceae plants / rice |
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| Research Abstract |
Exploiting the information on the genome sequences of rice and Arabidopsis, genes specifically expressed in anthers and those responsible for male sterility and fertility-restoration were analyzed using rice and Brassicaceae plants to develop novel techniques for F_1 hybrid breeding, which is presently the most reliable breeding method for improving plant productivity.
Three genes controlling exine production were newly identified, and isolated from Arabidopsis. Since mutants having nonfunctional alleles of these genes produce pollen grains lacking germination ability, identification of these genes enables the production of male-sterile lines having high seed productivity in Brassicaceae crops. Using a Brassica oleracea line having a fertility-restorer gene for mur-CMS (cytoplasmic male sterility), which was selected in the present study, we found orf72 to be a candidate of the mitochondrial gene responsible for mur-CMS. We also isolated the fertility-restorer gene for Boro-CMS in rice, Rf1, first in the world, and revealed that Rf1 protein is transferred to mitochondria and interacts with mitochondrial mRNA. Fertility-restorer genes for two other types of CMS in rice were mapped on rice chromosomes, and regions harboring these genes were delimited into short parts of chromosomes. Identification of these genes will be achieved soon. In the study of mutant genes responsible for nuclear-genic male sterility, we developed a reverse genetic mutant-selection technique using Brassica petiole extract and an original, simple, efficient, and cost-effective technique for SNP analysis. These techniques are expected to contribute to the development of a novel F_1 hybrid breeding method.
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Research Products
(47 results)
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[Journal Article] The HKM gene, which is identical to the MS1 gene of Arabidopsis thaliana, is essential for primexine formation and exine pattern formation.2005
Author(s)
Ariizumi T, Hatakeyama K, Hinata K, Sato, S, Kato, T, Tabata S, Toriyama K.
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Journal Title
Sex. Plant Reprod. 18
Pages: 1-7
Description
「研究成果報告書概要(和文)」より
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[Journal Article] The HKM gene, which is identical to the MS1 gene of Arabidopsis thaliana, is essential for primexine formation and exine pattern formation.2005
Author(s)
Ariizumi T, Hatakeyama K, Hinata K, Sato, S, Kate, T, Tabata S, Toriyama K
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Journal Title
Sex.Plant Reprod. 18
Pages: 1-7
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Disruption of the novel plant protein, NEF1, affects lipid accumulation in the plastids of the tapetum and exine formation of pollen resulting in male sterility in Arabidopsis thaliana.2004
Author(s)
Ariizumi T, Hatakeyama K, Hinata K, Inatsugi R, Nishida I, Sato S, Kato T, Tabata S, Toriyama K
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Journal Title
Plant J. 39
Pages: 170-181
Description
「研究成果報告書概要(和文)」より
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[Book] 遺伝学の基礎2006
Author(s)
西尾剛, 向井康比己, 大澤良, 草場信, 鳥山欽哉
Total Pages
170
Publisher
朝倉書店
Description
「研究成果報告書概要(和文)」より
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[Book] Basis of Genetics.2006
Author(s)
Nishio T, Mukai Y, Oosawa R, Kusaba M, Toriyama K
Total Pages
170
Publisher
Asakura Shoten
Description
「研究成果報告書概要(欧文)」より
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[Book] 植物遺伝学入門2004
Author(s)
三上哲夫, 西尾剛, 佐野芳雄, 遠藤隆, 大西近江
Total Pages
163
Publisher
朝倉書店
Description
「研究成果報告書概要(和文)」より
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