2004 Fiscal Year Final Research Report Summary
Identification and cloning of genes conditioning species-specific parasitism of Magnaporthe oryzae
| Project/Area Number |
14206006
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物保護
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| Research Institution | Kobe University |
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Principal Investigator |
MAYAMA Shigeyuki Kobe University, Faculty of Agriculture, Professor, 農学部, 教授 (00112251)
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| Co-Investigator(Kenkyū-buntansha) |
TOSA Yukio Kobe University, Graduate School of Science and Technology, Associate Professor, 自然科学研究科, 助教授 (20172158)
NAKAYASHIKI Hitoshi Kobe University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (50252804)
PYOYUN Park Kobe University, Graduate School of Science and Technology, Professor, 自然科学研究科, 教授 (20147094)
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| Project Period (FY) |
2002 – 2004
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| Keywords | Magnaporthe / Pyricularia |
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| Research Abstract |
To clarify genetic mechanisms of plant-species specificity of Magnaporthe oryzae, we crossed a Triticum isolate with a Setaria isolate, an Avena isolate, and an Oryza isolate. Using the F_1 progenies derived from these crosses, we identified PWT1, PWT2 (conditioning avirulence of the Setaria isolate on wheat), PWT3, PWT4 (conditioning avirulence of the Avena isolate on wheat), PWT5 (conditioning avirulence of the Oryza isolate on wheat), PFM1, PFM2 (conditioning avirulence of the Triticum isolate on foxtail millet), and PAT1 (conditioning avirulence of the Triticum isolate on oat). We also identified a resistance gene corresponding to PWT4, and designated it as Rwt4. Rwt4 corresponded to PWT4 in a one-to-one manner, suggesting that the plant species specificity of the Triticum/Avena isolates on wheat is conditioned by gene-for-gene interactions. From these avirulence genes, PWT1, PWT4, and PAT1 were chosen as targets for molecular cloning. Using bulked segregant analysis, we found molecular markers that are closely linked to PWT1, PWT4 and PAT1.
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