2004 Fiscal Year Final Research Report Summary
Studies on the Molecular Mechanism in Life-cycle Development and Signal Trnnsduction of Aphanomyces cochlioides, a Cause of Spinach Root Rot
| Project/Area Number |
14206013
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
TAHARA Satoshi Hokkaido Univ., Grad.School of Agric., Prof., 大学院・農学研究科, 教授 (50001475)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIHARA Teruhiko Hokkaido Univ., Grad.School of Agric., Prof., 大学院・農学研究科, 教授 (90002071)
HASHIDOKO Yasuyuki Hokkaido Univ., Grad.School of Agric., Asso.Prof., 大学院・農学研究科, 助教授 (40281795)
FUKUSHI Yukiharu Hokkaido Univ., Grad.School of Agric., Lecturer, 大学院・農学研究科, 講師 (60218906)
SAKIHAMA Yasuko Hokkaido Univ., Grad.School of Agric., Assis.Prof., 大学院・農学研究科, 助手 (10344491)
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| Project Period (FY) |
2002 – 2004
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| Keywords | Aphanomyces cochlioides / zoospore attractant / chemotaxis / phytopathogenic Oomycetes / host-specific attractant / signal transduction / receptor / life-cycle development |
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| Research Abstract |
1.The zoospores of phytopathogenic Oomycete, Aphanomyces cochlioides show clear positive chemotaxis to the host-specific singanl substances. The reputed receptor for the host-specific attractant toward the zoospores of spinach root rot pathogen, A.cochlioides wsa surveyed by using a biotin conjugated azidoflavone as a probe and resulted in 40 and 70 kDa membrane proteins on electropholetic gel plates.
2.The events caused by signal transduction regarding the life-cycle development of a Peronosporomycete (Oomycete) Aphanonyres cochlioides has been studied by using microscopic techniques. An interesting difference in F-actin profiles was observe between two kinds of custospores, one developing ahead to hyphal germination and the other rejuvenating to regeneration of zoospores.
3.About 150 rhizoplane bacteria were isolated from host and non-host plants, and our dual culture bioassay revealed that more than 5 % of isolates caused severe morphological alterations on phytopathogenic Oomycete Aphanomyces cochlioides and the fungal growth was strongly inhibited. From these results, we proposed that this method is new and effective way to find biological control agents against soilbome phytopathogens.
4.Cochliophilin A (5-hydroxy-6,7-methylenedioxyflavone) known as the host-specific zoospore attractant for a spinach root rot pathogen has been isolated so far only from Chenopodiaceae plants, spinach, sugar beet, and Chenopodium album, however, in our recent survey of zoospore attractants in Amamthaceae plant, Celosia cristata revealed that one of the active princilpes was indeed cochliophilin A.
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