2005 Fiscal Year Final Research Report Summary
Discovery of new DNA polymorphic markers and those applications to forensic medicine and humah genetics.
| Project/Area Number |
14207022
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | University of Fukui (2004-2005)
福井医科大学 (2002-2003) |
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Principal Investigator |
MATSUKI Takasumi University of Fukui, Faculty of Medicine, Dept.Forensic Medicine and Human Genetics, Professor, 医学部, 教授 (10126617)
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| Co-Investigator(Kenkyū-buntansha) |
IIDA Reiko University of Fukui, Faculty of Medicine, Dept.Forensic Medicine and Human Genetics, Assist.Prof, 医学部, 助手 (40139788)
TAKATSUKA Hisakazu University of Fukui, Faculty of Medicine, Dept.Forensic Medicine and Human Genetics, Assist.Prof, 医学部, 助手 (40242490)
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| Project Period (FY) |
2002 – 2005
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| Keywords | DXS10011 / Yfm1 / DYS441 / DYS442 / DYS443 / DYS444 / Mpv17-like protein / mitochondrial DNA |
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| Research Abstract |
1. Development and application of DNA polymorphic markers
We collected 869 chromosome data on our developed marker DXS10011, and found three new mutants.
Our developed DNA marker Yfm1 was localized on long arm of Y chromosome as a multi-copy polymorphic marker.
New five short tandem repeats markers on Y chromosome (DYS441, DYS442, DYS443, DYS444, DYS445) were found and developed multiplex PCR method. The multiplex PCR method for these STR markers was useful for forensic materials like old samples or mixed samples, and applicable on the distinction of the race from Japanese, white American and African American.
2. Development of age related markers for forensic medicine
We found new and functionally unknown gene named as M-LP (Mpv17-like protein) on mouse kidney and spleen by differential display method. The M-LP gene was expressed as age dependent manner, and possibly regulated activities and gene expressions of antioxidant enzymes from experiments of the M-LP gene expressions in COS-7 cells.
We developed a new method for the estimation of mitochondrial DNA (mtDNA) amounts on mouse organs. Amounts of mtDNA in bone marrow, spleen and lung were relatively low, and relatively high in heart and skeletal muscles. Amounts of mtDNA, translational products of mtDNA and protein expressions of mtDNA showed gradual increase according to aging in heart, lung, kidney, spleen and skeletal muscles.
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[Journal Article] Improvement of a multiplex PCR system for DYS441, DYS442, DYS443, DYS444 and DYS445, and a population study in 340 Japanese males.2005
Author(s)
Ogata Y, Yamaba T, Sawazaki K,.Iida R, Tsubota E, Ueki M, Takatsuka H, Matsuki T, Yasuda T, Kishi K
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Journal Title
Legal Med 7
Pages: 183-189
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A novel multiplex PCR system consisting of Y- STRs DYS441, DYS442, DYS443, DYS444 and DYS445.2003
Author(s)
Iida R, Sawazaki K, Ikeda H, Miyamoto T, Tsubota E, Takatsuka H, Masuyama M, Matsuki T, Yasuda T, Kishi K
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Journal Title
J Forensic Sci 48(5)
Pages: 1088-1090
Description
「研究成果報告書概要(欧文)」より
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[Book] DNA多型第13巻2005
Author(s)
小形泰宏 他
Total Pages
3(204-206)
Publisher
東洋書店
Description
「研究成果報告書概要(和文)」より
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[Book] DNA多型第12巻2004
Author(s)
坪田悦子 他
Total Pages
3(117-119)
Publisher
東洋書店
Description
「研究成果報告書概要(和文)」より
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[Book] DNA多型第11巻2003
Author(s)
澤崎一三 他
Total Pages
3(102-104)
Publisher
東洋書店
Description
「研究成果報告書概要(和文)」より
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[Book] DNA多型第11巻2003
Author(s)
飯田礼子 他
Total Pages
5(113-117)
Publisher
東洋書店
Description
「研究成果報告書概要(和文)」より
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[Book] DNA多型第10巻2002
Author(s)
飯田礼子 他
Total Pages
4(160-163)
Publisher
東洋書店
Description
「研究成果報告書概要(和文)」より
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[Book] DNA多型第10巻2002
Author(s)
松木孝澄 他
Total Pages
3(173-175)
Publisher
東洋書店
Description
「研究成果報告書概要(和文)」より
