Analysis of Mucosal Immunity by the Fc receptor for IgA and IgM

2004 Fiscal Year Final Research Report Summary

• Project/Area Number: 14207024
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 内科学一般
• Research Institution: University of Tsukuba
• Principal Investigator: SHIBUYA Akira University of Tsukuba, Department of Immunology, Professor, 大学院・人間総合科学研究科, 教授 (80216027)
• Project Period (FY): 2002 – 2004
• Keywords: Fcα / μ receptor / IgA / IgM / Paneth cell / Antibacteria / Fc receptor

Research Abstract

We previously identified an Fc receptor for IgA and IgM (Fcα/μ receptor), which is expressed on antigen presenting cells. We found that the Fcα/μ receptor mediated endocytosis of IgM immune complex with antigen, such as staphylococcus aureus, suggesting an important mechanisms of pathogen eradication by IgM. The present study aims to clarify a role of the Fcα/μ receptor in mucosal immunity. We have identified several isoforms of the soluble Fcα/μ receptor expressed on intestinal Paneth cells and epithelial cells of urinary tract. We found that the extracellular domain of the isoforms may have antimicrobial activity against E.coli. Furthermore, we observed that mice deficient in the Fcα/μ receptor gene exhibited high IgA concentration in both serum and fecus and the presence of many IgA producing cells in lamina proplia.
We also observed that the Fcα/μ receptor was strongly expressed on the area of germinal center. Immunohistochemical study revealed that FDC substantially expressed the Fcα/μ receptor in germinal center. These results suggest that the Fcα/μ receptor regulates IgA production in peyel's patches and/or intestinal flora by the soluble Fcα/μ receptor secreted from Paneth cells. Furthermore, we studied the mucosal immune responses by antigen challenge in mice deficient in the the Fcα/μreceptor gene.

Research Products

• [Journal Article] Requirement of the serine at residue 329 for lipid raft recruitmet of DNAM-1 (CD226) (2005)
  • Author(s): Shirakawa J, Shibuya K, Shibuya, A.
  • Journal Title: Int Immunol 17 Pages: 217-223
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Requirement of the tyrosines at residues 258 and 270 of MAIR-I in inhibitory effect on degranulation from basophilic leukemia RBL-2H3 (2005)
  • Author(s): Okoshi Y, et al.
  • Journal Title: Int Immunol 17 Pages: 65-72
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Requirement of the serine at residue 329 for lipid raft recruitment of DNAM-1 (CD226). (2005)
  • Author(s): Shirakawa J, Shibuya K, Shibuya A.
  • Journal Title: Int Immunol. 17 Pages: 217-223
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Requirement of the tyrosines at residues 258 and 270 of MAIR-1 in inhibitory effect on degranulation from basophilic leukemia RBL-2H3. (2005)
  • Author(s): Okoshi Y, Tahara-Hanaoka S, Nakahashi C, Honda S, Miyamoto A, Kojima H, Nagasawa T, Shibuya K, Shibuya A.
  • Journal Title: Int Immunol. 17 Pages: 65-72
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] DNAM-1(CD226), a two-sword fencer for innate and adaptive immunity. (2005)
  • Author(s): Shibuya A, Tahara-Hanaoka S, Shibuya K.
  • Journal Title: Curr Med Chem-AIAA. 4 Pages: 53-58
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] DNAM-1(CD226), a two-sword fencer for innate and adaptive immunity. (2004)
  • Author(s): Shibuya A, Tahara-Hanaoka S, Shibuya K.
  • Journal Title: Curr Med Chem-AIAA 4 Pages: 53-58
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Successful gene transfer into human CD4 positive T cells mediated by lentiviral vectors. (2004)
  • Author(s): Shibuya K, et al.
  • Journal Title: Immunology 12 Pages: 91-94
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Functional characterization of DNAM-1 (CD226) interaction with its ligands PVR (CD155) and nectin-2 (PRR-2/CD112) (2004)
  • Author(s): Tahara-Hanaoka S, et al.
  • Journal Title: Int Immunol 16 Pages: 533-538
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Treatment with alpha-galactosylceramide attenuates the development of bleomycin-induced pulmonary fibrosis (2004)
  • Author(s): Kimura T, et al.
  • Journal Title: J Immunol 172 Pages: 5782-5789
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Successful gene transfer into human CD4 positive T cells mediated by lentiviral vectors. (2004)
  • Author(s): Shibuya K, Miyoshi H, Wang Y, Kai H, Shibuya A.
  • Journal Title: Immunology. 12 Pages: 91-94
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional characterization of DNAM-1 (CD226) interaction with its ligands PVR (CD155) and nectin-2 (PRR-2/CD 112). (2004)
  • Author(s): Tahara-Hanaoka S, Shibuya K, Onoda Y, Zhang H, Yamazaki S, Miyamoto A, Honda SI, Lanier LL, Shibuya A.
  • Journal Title: Int Immunol. 16 Pages: 533-538
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Treatment with alpha-galactosylceramide attenuates the development of bleomycin-induced pulmonary fibrosis. (2004)
  • Author(s): Kimura T, Ishii Y, Morishima Y, Shibuya A, Shibuya K, Taniguchi M, Mochizuki M, Hegab AE, Sakamoto T, Nomura A, Sekizawa K.
  • Journal Title: J Immunol. 172 Pages: 5782-5789
  • Description: 「研究成果報告書概要(欧文)」より