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2004 Fiscal Year Final Research Report Summary

Study of Diagnosis, Epidemiology, Pathogenesis, Prevention and Treatment of Virus-induced Diarrhea

Research Project

Project/Area Number 14207037
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionThe University of Tokyo

Principal Investigator

USHIJIMA Hiroshi  University of Tokyo, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (10091068)

Co-Investigator(Kenkyū-buntansha) NISHIO Osamu  Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Director (Reseacher), 感染症情報センター第六室, 室長(研究職) (40270631)
Project Period (FY) 2002 – 2004
KeywordsJapan / Diagnosis / Immunochromatography / molecular epidemiology / multiplex PCR / norovirus / rotavirus
Research Abstract

(1)We succeeded to make 14 norovirus-like particles and a sapovirus-like particle by a genetic engineering method. Then, we developed firstly in the world an immunochromatographic method for detection of norovirus. We also developed EIA for this virus. (2)RT-multiplex PCRs to detect group A, B and C rotaviruses and adenovirus (set A), norovirus GI,GII, astrovirus and sapovirus (set B), hepatitis A and E viruses, enteroviruses and influenza A virus (set C) were designed and used to screen these viruses. These viruses detected were further characterized by genetic sequencing analyses. Moreover, food (shell fish) and water from river or sea were examined by real time PCR. It is found that this method is excellent with high sensitivity and specificity. It will be an indicator for food safety. (3)From the molecular epidemiology, the peak of prevalence of rotavirus was December or January over the past 20 years, however there is nowadays a shift of rotavirus incidence in March or April. A peak of norovirus infection is found in November or December known as winter. (4)The most prevalent rotavirus serotype G1 has been decreasing from 80-90% in 1990s to 10-30% in 2000s. Other serotyes such as G2,3,4 and 9 accounted for around 20-30%. By using monoclonal antibodies and sequence of rotavirus strains, subserotypes in intraserotype appeared. Note of worth was an outbreak of group C rotavirus suddenly appeared in Maizuru area. New genotypes and reassortant strains were recognized in norovirus and sapovirus. Human to human transmission, air-borne transmission and food-borne transmission were recognized in diarrheal viruses. (5)In the CSF of encephalopathy and encephalitis, rotavirus genome was often found. However, we could not clarify the mechanism of encephalopathy and encephalitis caused by this virus. SiRNA and photocatalyst (TiO_2) were recognized effective on rotavirus infection.

  • Research Products

    (69 results)

All 2005 2004 2003 2002 Other

All Journal Article (66 results) Book (3 results)

  • [Journal Article] Molecular Epidemiology of Adenovirus Infection among Pediatric Population with Diarrhea in Asia.2005

    • Author(s)
      Li L, et al.
    • Journal Title

      Microbiol Immunol 149(2)

      Pages: 121-128

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Genetic diversity of sapovirus in fecal specimens from infants and children with acute gastroenteritis in Pakistan.2005

    • Author(s)
      Phan TG, et al.
    • Journal Title

      Arch Virol 150(2)

      Pages: 371-377

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Outbreak of acute gastroenteritis associated with group A rotavirus and genogroup I sapovirus among adults in a mental health care facility in Japan.2005

    • Author(s)
      Yan H, et al.
    • Journal Title

      J Med Virol 75(3)

      Pages: 475-481

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Viral gastroenteritis in Mongolian infants.2005

    • Author(s)
      Hansman GS, et al.
    • Journal Title

      Emerg Infect Dis 11(1)

      Pages: 180-182

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cross-reactivity among sapovirus recombinant capsid proteins.2005

    • Author(s)
      Hansman GS, et al.
    • Journal Title

      Arch Virol 150(1)

      Pages: 21-36

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] わが国のA型ロタウイルスG血清型の動向-数ヶ所の病院・クリニックの継続的研究-2005

    • Author(s)
      牛島廣治 ほか
    • Journal Title

      病原微生物検出情報 26(1)

      Pages: 7-8

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Trend of group A rotavirus G serotypes : collaborating study of medical institutions in five areas in Japan,1984-2003.2005

    • Author(s)
      Ushijima H, et al.
    • Journal Title

      IASR. 26(1)

      Pages: 7-8

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characterizations of adenovirus type 41 isolates from children with acute gastroenteritis in Japan, Vietnam, and Korea.2004

    • Author(s)
      Li L, et al.
    • Journal Title

      J Clin Microbiol 42(9)

      Pages: 4032-4039

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Virus diversity and an outbreak of group C rotavirus among infants and children with diarrhea in Maizuru city, Japan during 2002-2003.2004

    • Author(s)
      Phan TG, et al.
    • Journal Title

      J Med Virol 74(1)

      Pages: 173-179

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Development of RT-multiplex PCR assay for detection of adenovirus and group A and C rotaviruses in diarrheal fecal specimens for children in China.2004

    • Author(s)
      Yan H, et al.
    • Journal Title

      Kansenshogakuzasshi 78(8)

      Pages: 699-709

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Detection of norovirus and sapovirus infection among children with gastroenteritis in Ho Chi Minh City, Vietnam.2004

    • Author(s)
      Hansman GS, et al.
    • Journal Title

      Arch Virol 149(9)

      Pages: 1673-1688

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Human astrovirus, Norovirus (GI, GII), and Sapovirus infections in Pakistani children with diarrhea.2004

    • Author(s)
      Phan TG, et al.
    • Journal Title

      J Med Virol 73(2)

      Pages: 256-261

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular epidemiology of viral gastroenteritis in Asia.2004

    • Author(s)
      Okitsu-Negishi S, et al.
    • Journal Title

      Pediatr Int 46(2)

      Pages: 245-252

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Foreward : Molecular epidemiology of viral infection in Asia.2004

    • Author(s)
      Ushijima H, et al.
    • Journal Title

      Pediatr Int 46(2)

      Pages: 202-206

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Genetic diversity of Norovirus and Sapovirus in hospitalized infants with sporadeic cases of acute gastroenteritis in Chiang Mai, Thailand.2004

    • Author(s)
      Hansman GS, et al.
    • Journal Title

      J Clin Microbiol 42

      Pages: 1305-1307

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ロタウイルス、アデノウイルス胃腸炎の迅速診断2004

    • Author(s)
      牛島廣治
    • Journal Title

      小児科 45(4)

      Pages: 652-655

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ウイルス性下痢症 感染性腸炎【各論】ウイルス感染と腸炎2004

    • Author(s)
      牛島廣治 ほか
    • Journal Title

      臨床消化器内科 19(8)

      Pages: 1129-1134

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] これから開発される国内外のワクチン2004

    • Author(s)
      牛島廣治 ほか
    • Journal Title

      小児内科 36(3)

      Pages: 398-401

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ロタウイルス感染症についての最近の疫学と知見2004

    • Author(s)
      牛島廣治 ほか
    • Journal Title

      小児科臨床 57

      Pages: 27-333

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Characterizations of adenovirus type 41 isolates from children withacute gastroenteritis in Japan, Vietnam, and Korea.2004

    • Author(s)
      Li L, et al.
    • Journal Title

      J Clin Microbiol 42(9)

      Pages: 4032-4039

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Detection of norovirus and sapovirus infection among children with gastroenteritis in Ho Chi Minn City, Vietnam.2004

    • Author(s)
      Hansman GS, et al.
    • Journal Title

      Arch Virol 149(9)

      Pages: 1673-1688

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Human astrovirus, Norovirus (GI,GII), and Sapovirus infections in Pakistani children with diarrhea.2004

    • Author(s)
      Phan TG, et al.
    • Journal Title

      J Med Virol 73(2)

      Pages: 256-261

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Rapid test of rotavirus and adenovirus gastroenteritis.2004

    • Author(s)
      Ushijima H.
    • Journal Title

      Shounika 45(4)

      Pages: 652-655

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Viral infection and gastroenteritis.2004

    • Author(s)
      Ushijima H, et al.
    • Journal Title

      Rinsho Shokakinaika 19(8)

      Pages: 1129-1134

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Developing vaccines in Japan and world.2004

    • Author(s)
      Ushijima H, et al.
    • Journal Title

      Shoninaika 36(3)

      Pages: 398-401

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Recent epidemiology and information of rotavirus Infection.2004

    • Author(s)
      Ushijima H, et al.
    • Journal Title

      Shonikarinsho 57(3)

      Pages: 327-333

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Genotype C of hepatitis B virus can be classified into at least two subgroups.2003

    • Author(s)
      Huy Tran TT, et al.
    • Journal Title

      J Gen Virol 85

      Pages: 283-292

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Establishment of an HIV cell-cell fusion assay by using two genetically modified HeLa cell lines and receptor gene.2003

    • Author(s)
      Sakamoto T, et al.
    • Journal Title

      J Virol Methods 114

      Pages: 159-166

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] High prevalence of hepatitis B virus pre-S mutant in countries where it is endemic and its relationship with genotype and chronicity.2003

    • Author(s)
      Huy T, TT, et al.
    • Journal Title

      J Clin Microbiol 41

      Pages: 5449-5455

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Detection of norovirus and sapovirus in fecal samples using reverse transcription single-round multiplex PCR.2003

    • Author(s)
      Yan H, Ushijima H et al.
    • Journal Title

      J Virol Methods 114

      Pages: 37-44

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Distribution of human rotaviruses, especially G9 strains in Japan from 1996 to 2000.2003

    • Author(s)
      Zhou Y, et al.
    • Journal Title

      Microbiol Immunol 47

      Pages: 591-599

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Evaluation of a newly developed immunochromatographic method for detection of Norovirus.2003

    • Author(s)
      Okame T, et al.
    • Journal Title

      Kansenshogaku Zasshi 77

      Pages: 637-639

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The development of sensitive latex agglutination tests for detecting astrovirus.2003

    • Author(s)
      Komoriya T, et al.
    • Journal Title

      JARMAN 13

      Pages: 103-114

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Prevalence of hepatitis virus types B through E and genotypic distribution of HBV and HCV in Ho Chi Minh city, Vietnam.2003

    • Author(s)
      Huy Tran TT, et al.
    • Journal Title

      Hepatology Research 26

      Pages: 275-280

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Emergence and disappearance of an immune molecule in basal Metazoa.2003

    • Author(s)
      Schroder HC, et al.
    • Journal Title

      J Biol Chem 278

      Pages: 32810-32817

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Recombination of genotype B and C in hepatitis B virus isolated from a Vietnamese patient with fulminant hepatitis.2003

    • Author(s)
      Huy Tran TT, et al.
    • Journal Title

      Jpn J Infect Dis 56

      Pages: 35-37

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Epidemiological features of rotavirus infection among hospitalized children with gastroenteritis in Ho Chi Minh city, Vietnam2003

    • Author(s)
      Doan LT, et al.
    • Journal Title

      J Med Virol 69

      Pages: 588-594

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Genetic characterization of adenovirus type 8 isolated in Hiroshima city.2003

    • Author(s)
      Adhikary AK, et al.
    • Journal Title

      J Clin Pathol 56

      Pages: 120-125

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Transient MR signal changes in the splenium of the corpus callosusm in rotavirus encephalopathy.2003

    • Author(s)
      Kobata R, et al.
    • Journal Title

      J Computor Assisted Tomography 26

      Pages: 825-828

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Detection, quantitation, and phylogenetic analysis of noroviruses in Japanese oyster.2003

    • Author(s)
      Nishida T, et al.
    • Journal Title

      Appl Environ Microbiol 69

      Pages: 5782-5786

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A food poisoning outbreak caused by purple Washington clam contaminated with norovirus and hepatitis A virus.2003

    • Author(s)
      Furuta T, et al.
    • Journal Title

      Kansenshogaku Zasshi 77

      Pages: 89-94

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Poliovirus uptake into and excretion from oysters.2003

    • Author(s)
      Fukuta M, et al.
    • Journal Title

      Kansenshogaku Zasshi 77

      Pages: 95-102

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ロタウイルス胃腸炎-新世紀の感染症学2003

    • Author(s)
      牛島 廣治
    • Journal Title

      日本臨床 61

      Pages: 156-161

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Detection of norovirus and sapovirus in fecal samples using reverse transcription single-round multiplex PCR.2003

    • Author(s)
      Yan H, et al.
    • Journal Title

      J Virol Methods 114

      Pages: 37-44

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ditribution of human rotaviruses, especially G9 strains in Japan from 1996 to 2000.2003

    • Author(s)
      Zhou Y, et al.
    • Journal Title

      Microbiol Immunol 47

      Pages: 591-599

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] MR signal changes in the splenium of the corpus callosusm in rotavirus encephalopathy.2003

    • Author(s)
      Kobata R, et al.
    • Journal Title

      J Computer Assisted Transient Tomography 26

      Pages: 825-828

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Poliovirus uptake into and excretion from oysters.2003

    • Author(s)
      Furuta T, et al.
    • Journal Title

      Kansenshogaku Zasshi 77

      Pages: 95-102

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Rotavirus gastroenteritis.2003

    • Author(s)
      Ushijima H.
    • Journal Title

      Nippon Rinsho 61

      Pages: 156-161

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Differenciation of serotypes B and E of human immunodeficiency virus type I by polymerase chain reaction using novel env gene primers.2002

    • Author(s)
      Yagyu F, et al.
    • Journal Title

      J Virol Method 10

      Pages: 11-20

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Outbreak of central nervous system disease associated with hand, foot, and mouth disease in Japan during the summer of 2000.2002

    • Author(s)
      Fujimoto T, et al.
    • Journal Title

      Microbiol Immunol 46(9)

      Pages: 621-627

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification and genotyping of Norwalk virus by stringent microplate hybridization of PCR products.2002

    • Author(s)
      Nishlo O.
    • Journal Title

      Nippon Rinsho 60(6)

      Pages: 1175-1180

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 下痢症ウイルスによる脳症-ウイルス感染に伴う脳炎・脳症-2002

    • Author(s)
      牛島 廣治
    • Journal Title

      臨床とウイルス 30(4)

      Pages: 242-248

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A群ロタウイルスの迅速診断-感染性胃腸炎-2002

    • Author(s)
      牛島 廣治
    • Journal Title

      総合臨床 51(11)

      Pages: 2954-2958

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ノーウォークウイルスの分子疫学2002

    • Author(s)
      牛島 廣治
    • Journal Title

      日本臨床 60(6)

      Pages: 1143-1147

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification and genotyping of Norwalk virus by stringent microplate hybridization of PCR products.2002

    • Author(s)
      Nishio, O et al.
    • Journal Title

      Nippon Rinsho 60(6)

      Pages: 1175-1180

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Encephalopathy caused by diarrheal viruses.2002

    • Author(s)
      Ushijima H.
    • Journal Title

      Rinsho to uirusu 30(4)

      Pages: 242-248

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Rapid diagnosis of group A rotavirus.2002

    • Author(s)
      Ushijima H.
    • Journal Title

      Sogo Rinsho 51(11)

      Pages: 2954-2958

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Molecular epidemiology of Norwalk virus.2002

    • Author(s)
      Ushijima H.
    • Journal Title

      Nippon Rinsho 60(6)

      Pages: 1143-1147

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Etiologic agents of acute gastroenteritis among Japanese infants and children : virus diversity and genetic analysis of sapovirus.

    • Author(s)
      Phan TG, et al.
    • Journal Title

      Arch Virol (In press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Characterization of polyclonal antibodies raised against five virus-like particles.

    • Author(s)
      Hansman GS, et al.
    • Journal Title

      Arch Virol (In press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Proteolytic processing of sapovirus ORF1 polyprotein.

    • Author(s)
      Oka T, et al.
    • Journal Title

      J Virol (In press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A novel RT-multiplex PCR for enteroviruses, hepatitis A and E viruses and influenza A virus among infants and children with diarrhea in Vietnam.

    • Author(s)
      Phan TG, et al.
    • Journal Title

      Arch Virol (In press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Etiologic agents of acute gastroenteritis among Japanese infants and children : virus diversity and genetic analysis of sapovirus.

    • Author(s)
      Phan TG, et al.
    • Journal Title

      Arch Virol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characterization of polyclonal antibodies raised against five virus-like particles.

    • Author(s)
      Hansman GS, et al.
    • Journal Title

      Arch Virol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Proteolytic processing of sapovirus ORF 1 polyprotein.

    • Author(s)
      Oka T, et al.
    • Journal Title

      J Virol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A novel RT-multiplex PCR for enteroviruses, hepatitis A and E viruses and influenza A virus among infants and children with diarrhea in Vietnam.

    • Author(s)
      Phan TG, et al.
    • Journal Title

      Arch Virol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 小児科学 改訂第9版2004

    • Author(s)
      五十嵐隆 編集
    • Total Pages
      1112
    • Publisher
      文光堂
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 疾患・症状別 今日の治療と看護 改訂第2版2004

    • Author(s)
      水島裕, 黒川清 総編集
    • Total Pages
      1586
    • Publisher
      南江堂
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 予防接種ノート2003

    • Author(s)
      牛島廣治 ほか
    • Total Pages
      290
    • Publisher
      診断と治療社
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2006-07-11  

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