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2005 Fiscal Year Final Research Report Summary

IMPLICATION OF ANGIOGENIC FACTORS IN FEMALE REPRODUCTIVE TRACT

Research Project

Project/Area Number 14207064
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

TAKETANI Yuji  The University of Tokyo, Faculty of Medicine, Professor, 医学部附属病院, 教授 (10114539)

Co-Investigator(Kenkyū-buntansha) YANO Tetsu  The University of Tokyo, Faculty of Medicine, Associate Professor, 医学部附属病院, 助教授 (90251264)
FUJII Tomoyuki  The University of Tokyo, Faculty of Medicine, Associate Professor, 医学部附属病院, 助教授 (40209010)
KUGU Koji  The University of Tokyo, Faculty of Medicine, Lecturer, 医学部附属病院, 講師 (30322051)
MOMOEDA Mikio  The University of Tokyo, Faculty of Medicine, Lecturer, 医学部附属病院, 講師 (50221627)
OSUGA Yutaka  The University of Tokyo, Faculty of Medicine, Lecturer, 医学部附属病院, 講師 (80260496)
Project Period (FY) 2002 – 2005
Keywordsadiponectin / endometrium / endometriosis / implantation / receptor / AMPkinase
Research Abstract

Adiponectin, a pleiotropic cytokine, exerts its effects via the specific receptors, Adipo R1 and Adipo R2. Whereas circulating adiponectin concentrations decrease in women with endometriosis and endometrial cancer, possible effects of adiponectin and the presence of the receptors in the endometrium have not been determined. In this study, we examined the expression of adiponectin receptors, Adipo R1 and Adipo R2 in the human endometrium, and assessed effects of adiponectin in the endometrial cells. Expression of Adipo R1 and Adipo R2 in the endometrial tissues was evaluated by real-time quantitative PCR, in situ hybridization, and Western blotting. The effects of adiponectin on phosphorylation of AMP-activate protein kinase (AMPK), a regulator of energy homeostasis, in cultured endometrial stromal cells (ESCs) and epithelial cells (EECs) were studied by Western blotting. The effects of adiponectin on IL-1b-induced secretion of IL-6,IL-8 and MCP-1 from cultured ESCs were determined using specific ELISAs. The expression of Adipo R1 and Adipo R2 was detected in the endometrium. The expression of both genes was increased in the mid-luteal phase, the period of embryo implantation. In situ hybridization revealed that both Adipo R1 and Adipo R2 appeared to be equally expressed in the epithelial cells and in the stromal cells. Adiponectin increased phosphorylation of AMPK in ESCs and EECs. Adiponectin decreased IL-1b-induced secretion of IL-6,IL-8 and MCP-1 from ESCs. These findings suggest that adiponectin exerts energy-homeostatic and anti-inflammatory effects in the endometrium, and these effects might be relevant to pathological and physiological endometrium-related events such as implantation and endometriosis.

  • Research Products

    (13 results)

All 2006 2005 Other

All Journal Article (13 results)

  • [Journal Article] Mechanical stretch upregulates IGFBP-1 secretion from decidualized endometrial stromal cells.2006

    • Author(s)
      Harada M, Osuga Y, Takemura Y, Yoshino O, Koga K, Hirota Y, Hirata T, Morimoto C, Yano T, Taketani Y
    • Journal Title

      Am J Physiol Endocrinol Metab 290

      Pages: E268-E272

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mechanical stretch upregulates IGFBP-1 secretion from decidualized endometrial stromal cells.2006

    • Author(s)
      Harada M, Osuga Y, Takemura Y, Yoshino O, Koga K, hirata T, Morimura C, Yano T, Yano T, Taketani Y.
    • Journal Title

      Am J Physiol Endocrinol Metad 290

      Pages: E268-E272

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Concentration of adiponectin in peritoneal fluid is decreased in women with endometriosis2005

    • Author(s)
      Takemura Y, Osuga Y, Harada M, Hirata T, Koga K, Yoshino O, Hirota Y, Morimoto C, Yano T, Taketani Y
    • Journal Title

      Am J Reprod Immunol 54

      Pages: 217-221

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Serum adiponectin concentrations are decreased in women with endometriosis2005

    • Author(s)
      Takemura Y, Osuga Y, Harada M, Hirata T, Koga K, Morimoto C, Hirota Y, Yoshino O, et al.
    • Journal Title

      Hum Reprod 20

      Pages: 3510-3513

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] GnRH II as a possible cytostatic regulator in the development of endometriosis2005

    • Author(s)
      Morimoto C, Osuga Y, Yano T, Takemura Y, Harada M, Hirata T, Hirota Y, Yoshino O, Koga K, Kugu K, Taketani
    • Journal Title

      Hum Reprod 20

      Pages: 3212-3218

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Possible involvement of thrombin/protease-activated receptor I system in the pathogenesis of endometriosis2005

    • Author(s)
      Hirota Y, Osuga Y, Hirata T, Yoshino O, Koga K, Harada M, Morimoto C, Nose E, et al.
    • Journal Title

      J Clin Endocrinol Metab 90

      Pages: 3673-3679

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Activation of protease-activated receptor 2 stimulates proliferation and interleukin(IL)-6 and IL-8 secretion of endometriotic stromal cells.2005

    • Author(s)
      Hirota Y, Osuga Y, Hirata T, Harada M, Morimoto C, Yoshino O, Koga K, Yano T, Tsutsumi O, Taketani Y
    • Journal Title

      Hum Reprod 20

      Pages: 3547-3553

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Concentration of adiponectin in peritoncalb.2005

    • Author(s)
      Harada M, Osuga Y, Takemura Y, Yoshino O, Koga K, hirata T, Morimura C, Yano T, Yano T, Taketani Y.
    • Journal Title

      Am J Physiol Immunol 54

      Pages: 217-221

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Serum sdipon concontrreasim2005

    • Author(s)
      Harada M, Osuga Y, Takemura Y, Yoshino O, Koga K, hirata T, Morimura C, Yano T, Yano T, Taketani Y.
    • Journal Title

      Hum Reprtod 20

      Pages: 3510-3513

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] GnRH II as a possible cytostatio regulztor in the development of endometriosis.2005

    • Author(s)
      Harada M, Osuga Y, Takemura Y, Yoshino O, Koga K, hirata T, Morimura C, Yano T, Yano T, Taketani Y.
    • Journal Title

      Hum Reprtod 20

      Pages: 3212-3218

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Possible involvement of thrombin/protease-activated receptor I system in the pathogenesis of Endometriosis.2005

    • Author(s)
      Harada M, Osuga Y, Takemura Y, Yoshino O, Koga K, hirata T, Morimura C, Yano T, Yano T, Taketani Y.
    • Journal Title

      J Clin Endocrinol Metab 90

      Pages: 3673-3679

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Activation of protease-activated receptor 2 stimulates proliferation and interleukin (IL)-6 and IL-8 secretion of endometriotic stromai ceiis.2005

    • Author(s)
      Harada M, Osuga Y, Takemura Y, Yoshino O, Koga K, hirata T, Morimura C, Yano T, Yano T, Taketani Y.
    • Journal Title

      Hun Reprod 20

      Pages: 3547-3553

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 「研究成果報告書概要(和文)」より

    • Author(s)
      Y

URL: 

Published: 2007-12-13  

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