2005 Fiscal Year Final Research Report Summary
Regulatory mechanisms and interactions of extracellular molecules
| Project/Area Number |
14207076
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
YANAGISHITA Masaki Tokyo Medical and Dental University, Graduate School, Biochemistry, Professor, 大学院・医歯学総合研究科, 教授 (70132793)
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| Co-Investigator(Kenkyū-buntansha) |
SHINOMURA Tamayuki Tokyo Medical and Dental University, Associate Professor, 大学院・医歯学総合研究科, 助教授 (70206118)
YOKOYAMA Miki Tokyo Medical and Dental University, Associate Professor, 大学院・医歯学総合研究科, 助教授 (70191533)
INOUE Katarzyna Anna Tokyo Medical and Dental University, Faculty of Dentistry, Educational Stuff, 歯学部, 教務職員 (90302877)
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| Project Period (FY) |
2002 – 2005
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| Keywords | extracellular matrix / proteoglycan / heparan sulfate / membrane domain / molecular interactions / degrading enzyme / complex carbohydrates |
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| Research Abstract |
In addition
to providing mechanical supports for various body structures in animals,
extracellular matrix also provides living environments for cells. Thus, it is involved in many crucial biological functions, including cell differentiation, proliferation, metabolism and regulated responses to environmental stimuli. Especially biological signals coming from outside cells are very important regulating fundamental cell functions. We have especially focused our attention on the analysis of molecular interactions between extracellular matrix molecules cellular molecules occurring at the cell surface, as a pertinent experimental system studying functional significance of extracellular matrix molecules. Specifically, a hypothesis that specialized plasma membrane domains are involved in the molecular interactions with extracellular matrix. A detergent-resistant plasma membrane preparation was made by solubilizing cell cultures with Triton X-100 followed by ultracentrifugation in a sucrose gradient. The presence of a specialized membrane domain was identified by colocalization of proteins, such as CD 38 and Lyn. A membrane domain preparation isolated from parathyroid cell sutures was examined by Western blot analyses probed with antibodies against heparan sulfate proteoglycans, which indicated significant enrichment syndecan-4 heparan sulfate proteoglycan and exclusion of syndecan-1. Further confirmation using MALDI-TOF mass spectroscopy has been on the way. Experiments also suggested that heparan sulfate proteoglycans participate in the structural stability of membrane domains. Effects of heparan sulfate degrading enzymes on the formation of membrane domains in the cell cultures in vitro are being studied. Future experiments will include detailed examination how heparan sulfate proteoglycans may be involved in the signal transduction and how they are contributing to the mechanical integrity of plasma membranes.
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[Journal Article] Versican/PG-M regulates chondrogenesis as an extracellular matrix molecule crucial for mesenchymal condensation,2006
Author(s)
Kamiya, N., Watanabe, H., Habuchi, H., Takagi, H, Shinomura, T., Shimizu, K., Kimata, K.
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Journal Title
J.Biol.Chem. 281
Pages: 2390-2400
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Syndecan-1 Expression during the Formation of Junctional Epithelium,2005
Author(s)
Dias, R.A., Shibata, S., Hashimoto-Uoshima, M., Podyma-Inoue, K.A., Ishikawa, I., Yanagishita, M.
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Journal Title
J.Periodontol. 76
Pages: 696-704
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Basement membrane matrix modifies cytokine interactions between lung cancer cells and fibroblasts.2005
Author(s)
Akashi, T., Minami, J., Ishige, Y., Eishi, Y., Takizawa, T., Koike, M., Yanagishita, M.
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Journal Title
Pathobiology 72
Pages: 250-259
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of genes preferentially expressed in Articular Cartilage by Suppression Subtractive Hybridization,2005
Author(s)
Yoshimi, M, Miyaishi, O., Nakamura, S., Shirasawa, S., Kamochi, H., Miyatani, S., Ikawa, Y., Shinomura, T.
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Journal Title
J.Med.Dent.Sci. 52
Pages: 203-211
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of genes preferentially expressed in periodontal ligament : Specific expression of a novel secreted protein, FDC-SP,2005
Author(s)
Nakamura, S., Terashima, T., Yoshida, T., Iseki, S., Takano, Y., Ishikawa, I., Shinomura, T.
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Journal Title
Biochem.Biophys.Res.Commun. 338
Pages: 1197-1203
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Gene trap screening for cell surface and extracellular matrix molecules produced by chondrocytes,2005
Author(s)
Shirasawa, S., Yoshimi, M., Kamochi, H., Muneta, T., Kimura, J.H., Hook, M., Shinomura, T.
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Journal Title
J.Biochem. 137
Pages: 79-85
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Charge-based separation of detergent-resistant membranes of mouse splenic B cells.2004
Author(s)
Katsumata, O., Kimura, T., Nagatsuka, Y., Hirabayashi, Y., Sugiya, H., Furuyama, S., Yanagishita, M., Hara-Yokoyama, M.
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Journal Title
Biochem.Biophys.Res.Commun. 319
Pages: 826-831
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Carbohydrate-dependent signaling from the phosphatidylglucoside-based microdomain induces granulocytic differentiation of HL60 cells,2003
Author(s)
Yasuko Nagatsuka, Miki Hara-Yokoyama, Takeshi Kasama, Masataka Takekoshi, Fumiko Maeda, Seiji Ihara, Shigeyoshi Fujiwara, Eriko Ohshima, Kumiko Ishii, Toshihide Kobayashi, Kazufumi Shimizu, Yoshio Hirabayashi
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Journal Title
Proc.Natl.Acad.Sci.U.S.A. 100
Pages: 7454-7459
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Heparanase activities and the tumor invasion patterns inhuman squamous cell carcinoma xenografts,2003
Author(s)
Kurokawa, H., Katsube, K., Podyma, K.A., Ikuta, M., Iseki, H., Jian, D., Nakajima, M., Akashi, T., Omura, K., Yanagishita, M.
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Journal Title
Cancer Science 94
Pages: 277-285
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Novel sulfated gangliosides, high-affinity ligands for neural siglecs, inhibit NADase activity of leukocyte cell surface antigen CD38,2003
Author(s)
Hara-Yokoyama, M., Ito, H., Ueno-Noto, K., Takano, K., Ishida H., Kiso, M.
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Journal Title
Bioorganic and Medicinal Chemistry Letter 13
Pages: 3441-3445
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Distinct interaction of versican/PG-M with hyaluronan and link protein,2003
Author(s)
Matsumoto, K., Shionyu, M., Go, M., Shimizu, K., Shinomura, T., Kimata, K., Watanabe, H.
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Journal Title
J.Biol.Chem. 278
Pages: 41205-41212
Description
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[Journal Article] Oligomers of Glycamino Acid.2002
Author(s)
Suhara, Y., Yamaguchi, Y., Collins, B., Schnaar, R.L., Yanagishita, M., Hildreth, J.E.K., Shimada, I., Ichikawa, Y.
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Journal Title
Bioorganic & Medicinal Chemistry 10
Pages: 1999-2013
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effects of interleukin-4 on proteoglycan accumulation in human gingival fibroblasts.2002
Author(s)
Hashimoto-Uoshima, M., Noguchi, M., Suzuki, K., Murata, A., Yanagishita, M., Ishikawa, I.
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Journal Title
J.Periodont.Res. 37
Pages: 42-49
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characterization of an Anti-Decorin Monoclonal Antibody, and Its Utility,2002
Author(s)
Sawada, H., Shinomura, T., Kimata, K., Takeuchi, J., Tsuji, T., Watanabe, H.
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Journal Title
J.Biochem. 132
Pages: 997-1002
Description
「研究成果報告書概要(欧文)」より
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