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2003 Fiscal Year Final Research Report Summary

Functions of transcription elongation factor S-II for cell stress response and development

Research Project

Project/Area Number 14207097
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionThe University of Tokyo

Principal Investigator

SEKIMIZU Kazuhisa  The University of Tokyo, Graduate School of Pharmaceutical Sciences, Professor, 大学院・薬学系研究科, 教授 (90126095)

Co-Investigator(Kenkyū-buntansha) ITOH Takahiro  The University of Tokyo, Graduate School of Pharmaceutical Sciences, Research Associate, 大学院・薬学系研究科, 助手 (00323452)
Project Period (FY) 2002 – 2003
Keywordstranscription factor / S-II / RNA polymerase II / development / gene knock-out / yeast cells / yeast two hybrid method / erythro differentiation
Research Abstract

S-II was originally identified as a stimulatory factor of RNA polymerase II in vitro. We have been studying biology of this protein for more than 30 years. Nowadays, S-II is recognized as a transcription elongation factor ubiquitously present in eukaryotic cells. A number of researchers are now studying a role of S-II in eukaryotic transcription. It has been proposed that 5-11 plays an important role on the regulation of transcription in eukaryotic cells. However, there is no direct evidence supporting this notion.
The purpose of this project was to test a hypothesis that. S-II plays a role in oxidative stress response in cells. We also hypothesized that S-II is essential for development. We examined phenotypes of yeast cells and mice whose S-II genes were deleted by homologous recombination technique. We showed that yeast deletion mutant of the S-II gene showed higher sensitivity to oxidative stress. We also demonstrated that fidelity of transcription decreased in the mutant. Oxidative stress may cause oxidization of nucleotides that are substrates for RNA synthesis. Furthermore, embryos of mice deletion mutant of the S-II gene showed lethality at early stage of development. We found that the mutant showed abnormal phenotype in erythro differentiation. These results supports our hypothesis that S-II is important for tolerance to oxidative stress and is essential for development of individual bodies.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] T.Ubukata, T.Shimizu, N.Adachi, K.Sekimizu, T.Nakanishi: "Cleavage, but not read-through, stimulation activity is responsible for three biologic functions of transcription elongation factor S-II."J Biol Chem. 278. 8580-8585 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Saso, T.Ito, S.Natori, K.Sekimizu: "Identification of a novel tissue-specific transcriptional activator FESTA as a protein that interacts with the transcription elongation factor S-II."J Biochem. 133. 493-500 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Koyama, T.Ito, T.Nakanishi, N.Kawamura, K.Sekimizu: "Transcription elongation factor S-II maintains transcriptional fidelity and confers oxidative stress resistance."Genes Cells. 8. 779-788 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Nakanishi, K.Sekimizu: "SDT1/SSM1, a multicopy suppressor of S-II null mutant, encodes a novel pyrimidine 5'-nucleotidase."J Biol Chem. 277. 22103-22106 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Ubukata, T.Shimizu, N.Adachi, K.Sekimizu, T.Nakanishi: "Cleavage, but not read-through, stimulation activity is responsible for three biologic functions of transcription elongation factor S-II"J Biol Chem. 278. 8580-8585 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Saso, T.Ito, S.Natori, K.Sekimizu: "Identification of a novel tissue-specific transcriptional activator FESTA as a protein that interacts with the transcription elongation factor S-II"J Biochem (Tokyo). 133. 493-500 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Koyama, T.Ito, T.Nakanishi, N.Kawamura, K.Sekimizu: "Transcription elongation factor S-II maintains transcriptional fidelity and confers oxidative stress resistance"Genes Cells. 8. 779-788 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Nakanishi, K.Sekimizu: "SDT1/SSM1, a multicopy suppressor of S-II null mutant, encodes a novel pyrimidine 5'-nucleotidase"J Biol Chem. 277. 22103-22106 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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