2004 Fiscal Year Final Research Report Summary
A full-life screen of mutations in internal organs in the see-through medaka
| Project/Area Number |
14209009
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
広領域
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| Research Institution | Nagoya University |
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Principal Investigator |
WAKAMATSU Yuko Nagoya University, Bioscience and Biotechnology Center, Professor, 生物機能開発利用研究センター, 教授 (20026800)
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| Co-Investigator(Kenkyū-buntansha) |
SEIKI Makoto JST, SORST Project, Kondo Research Team, Team Leader, SORSTプロジェクト・近藤研究チーム, チームリーダー
HASHIMOTO Hisashi Nagoya University, Bioscience and Biotechnology Center, Assistant Professor, 生物機能開発利用研究センター, 助手 (30359757)
OZATO Kenjiro Nagoya University, Honorary Professor, 名誉教授
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| Project Period (FY) |
2002 – 2004
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| Keywords | mutagenesis / ENU / dominant mutation / recessive mutation / enlargement in the kidney / inversion in heart structure / enlargement in the heart |
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| Research Abstract |
The see-through medaka is transparent in the body wall and internal organs can be observed from outside in adult fish without dissection. To develop disease models, we carried out ENU mutagenesis in this medaka strain STII, to screen dominant and recessive mutations in adult internal organs.
Dominant mutations : Twenty-five candidates of dominant mutations were found in the F_1 generation. Nine showed enlargement or abnormal morphology in the heart, nine an enlargement morphology in the kidney, six They showed affecabnormality in the tissue color in the liver, and one popped eyes. Seventeen of them produced F_2 progenies in the crossing with STII. By histological studies carried out in the progenies with the affected phenotypes, one mutant showing an enlargement of the kidney tissues, two with an enlarged heart in addition to tumorous tissues in the kidney and liver, one with an enlarged heart, and one with an enlarged heart showing an abnormal position of the atrium and ventricle.
Recessive mutations : Six mutations were confirmed in the Fs generation, that is, three mutations with enlarged kidney, one which lacks an eye vesicle in the one side, and two showing abnormal laterality.
From these results, it became clear that the see-through medaka is a good material to screen mutations in internal organs in adult animals. Especially, the screening of mutations in the kidney in this medaka stock was effective because the tissue was easy to observe in the living fish and individuals with mutations in this organ generally can be survive for long time. The phenotype in the polycystic kidney disease in a medaka natural mutant, pc, was described to compare that in these mutations in kidney.
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[Journal Article] Fish mesonephric model of polycystic kidney disease in medaka(Oryzias latipes) pc mutant.2005
Author(s)
Mochizuki, E., Fukuta, K., Tada, T., Harada, T., Watanabe, N., Matsuo, S., Hashimoto, H., Ozato, K., Wakamatsu, Y.
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Journal Title
Kidney International 68
Pages: 1-12
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The tyrosinase gene of the i^b albino mutant of the medaka fish carries a transposable element insertion in the promoter region.2004
Author(s)
Iida, A., Inagaki, H., Suzuki, M., Wakamatsu, Y., Hori, H., Koga, A.
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Journal Title
Pigment Cell Res. 17
Pages: 158-164
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The Tomita collection of medaka pigmentation mutants as a resource for understanding neural crest cell development.2004
Author(s)
Kelsh, R.N., Inoue, C., Momoi, A., Furutani-Seiki, M., Kondoh, R, Ozato, K., Wakamatsu, Y.
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Journal Title
Mech.Dev. 121(7-8)
Pages: 841-859
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Possible roles of zic1 and zic4, identified within the medaka Double anal fin (Da) locus, in dorsoventral patterning of the trunk-tail region (related to phenotypes of the Da mutant).2004
Author(s)
Ohtsuka, M., Kikuchi, N., Yokoi, H., Kinoshita, M., Wakamatsu, Y., Ozato, K., Wittbrodt, J., Takeda, H., Inoko, H., Kimura, M.
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Journal Title
Mech.Dev. 121(7-8)
Pages: 873-882
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Conserved function of medaka pink-eyed dilution in melanin synthesis and its divergent transcriptional regulation in gonads among vertebrates.2004
Author(s)
Fukamachi, S., Asakawa, S., Wakamatsu, Y., Shimizu, N., Mitani, H., Shima, A.
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Journal Title
Genetics 168
Pages: 1519-1527
Description
「研究成果報告書概要(欧文)」より
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