2004 Fiscal Year Final Research Report Summary
Optical Recording and Analysis of Neural Activities in Odor response of Larva Silkworm Moth
| Project/Area Number |
14350437
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物・生体工学
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| Research Institution | Osaka University (2003-2004)
Hiroshima University (2002) |
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Principal Investigator |
OHTAKE Hisao Osaka University, Department of Biotechnology, Professor, 大学院・工学研究科, 教授 (10127483)
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| Co-Investigator(Kenkyū-buntansha) |
TAKIGUCHI Noboru Hiroshima University, Department of Molecular Biotechnology, Assistant, 大学院・先端物質科学研究科, 助手 (20304462)
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| Project Period (FY) |
2002 – 2004
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| Keywords | Silkworm moth / Olfactory / Mulberry tree / Electrophysiological experiments |
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| Research Abstract |
One approach to the study of massed neural activity has been to use fluorescent voltage-sensitive dyes to track the neural activity of many neurons at once. The voltage-sensitive dye is first applied to the neurons of the olfactory bulb. The bulb is then illuminated with ultraviolet light, and video images of the exposed olfactory bulb are taken through a microscope. The UV light stimulates the voltage-sensitive dye to give off light when the cell in which it is located depolarizes. By using a computer to subtract the background image from the image obtained when the odor is present. We made optical recordings in vivo in the silkworm moth brain to investigate neuronal population responses to odorants delivered to the animal. Adult male silkworm moths were used within 2-4 days after eclosion. After anesthesia by cooling at 4℃ for about 30 min, the head capsule was opened above the brain, and most of the muscles in the head were removed. The brain was desheathed by fine tweezers and stained with a voltage-sensitive dye RH414 (Molecular probe). The RH414 dye was dissolved in a physiological saline at a concentration of 3 mg/ml. Excess dye was washed off with the saline. The signals were superimposed on the bright-field image of AL to correlate the responses directly with the AL structure. Odors evoked specific spatio-temporal excitation patterns in the AL. Specific ensembles of active glomeruli are likely to represent odors in a combinatorial manner. Odor blends evoked patterns that were combinations of the single odorant responses. Odors are not only represented as spatial activity patterns, but as patterns that evolve over time.
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