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2004 Fiscal Year Final Research Report Summary

Photosynthesis and Biomass Improvement in Rice

Research Project

Project/Area Number 14360036
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionTohoku University

Principal Investigator

MAKINO Amane  Tohoku University, Graduate School of Agricultural Science, Associate Professor, 大学院・農学研究科, 助教授 (70181617)

Project Period (FY) 2002 – 2004
KeywordsPhotosynthesis / Rice / Oryza sativa L. / Rubisco / Gas exchange / Carbon assimilation / transgenic plants / electron transport
Research Abstract

Transgenic rice (Oryza sativa L.) plants with different amounts of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) were obtained by the Agrobacterium-mediated transformation with the rice rbcS sense or antisense gene under the control of the rice rbcS promoter. Although Rubisco content was enhanced up to 150% wild type Rubisco in some sense-rbcS transformant, the analyses of the response of CO_2 assimilation to intercellular CO_2 partial pressure (A : Ci curve) did not show that enhancement of Rubisco content leads to an increase in CO_2-limited photosynthesis. In addition, several the sense transformant lines show decreased photosynthesis at 100 Pa CO_2. This was caused by partial deactivation of Rubisco in vivo.
For the anti-rbcS plants, changes in the levels of protein and rbcL and rbcS mRNA of Rubisco and in the activities other several Calvin-cycle enzymes were examined in the leaves at different leaf positions. A decrease in Rubisco content always led to decreased photosynthesis, especially under low CO_2 conditions. Although no differences the activities of other enzymes and chlorophyll content were found between genotypes, only Rubisco content in leaves at lower positions remained relatively higher levels in antisense plants. Since rbcS mRNA levels were not detected in their leaves, these results indicate that suppression of Rubisco degradation occurred in the senescent leaves of the antisense plants. Thus, the existence of a specific degradation mechanism(s) of Rubisco during leaf senescence is suggested to be in vivo.

  • Research Products

    (12 results)

All 2005 2004

All Journal Article (10 results) Book (2 results)

  • [Journal Article] Response off rice to p(Co_2) enrichment. The relationship between photosynthesis and nitrogen metabolism.2005

    • Author(s)
      Amane Makinoを含む他3名
    • Journal Title

      Journal of Crop Improvement 13

      Pages: 31-53

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The photosynthetic properties of rice leaves treated with low temperature and high irradiance.2005

    • Author(s)
      Amane Makinoを含む他3名
    • Journal Title

      Plant and Cell Physiology 46

      Pages: 1377-1383

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of nitrogen nutrition on the relationships between the levels of rbcS and rbcL mRNAs and the amount of ribulose-1, 5-bisphosphate carboxylase/oxgenase synthesized in the eighth leaves of rice from emergence through senescence.2005

    • Author(s)
      Amane Makinoを含む他3名
    • Journal Title

      Plant, Cell and Environment 28(in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Response of rice to p(CO_2) enrichment. The relationship between photosynthesis and nitrogen metabolism.2005

    • Author(s)
      S.Seneweera*, A.Makino, T.Mae, A.S.Basra
    • Journal Title

      J Crop Improvement. 13

      Pages: 31-53

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The photosynthetic properties of rice leaves treated with low temperature and high irradiance2005

    • Author(s)
      N.Hirotsu, A Makino*, S.Yokota, T.Mae
    • Journal Title

      Plant Cell Physiol. 46

      Pages: 1377-1383

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effects of nitrogen nutrition on the relationships between the levels of rbcS and rbcL mRNAs and the amount of ribulose-1,5-bisphosphate carboxylase/oxygenase synthesized in the eighth leaves of rice from emergence through senescence.2005

    • Author(s)
      K.Imai, Y.Suzuki, A.Makino, T.Mae*
    • Journal Title

      Plant Cell Environ 28(in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The amount of ribulose-1, 5-bisphoshate carboxylaseoxygenase (Rubisco) protein and levels of mRNAs of rbcS and rbcL in the leaves at different positions in transgenic rice plants with decreased content of Rubisco2004

    • Author(s)
      Amane Makinoを含む他3名
    • Journal Title

      Soil Science and Plant Nutrition 50

      Pages: 233-139

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Changes in the thermal dissipation and the electron flow in the water-water cycle in rice grown under conditions of physiologically low temperature.2004

    • Author(s)
      Amane Makinoを含む他3名
    • Journal Title

      Plant and Cell Physiology 45

      Pages: 635-644

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The amount of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) protein and levels of mRNAs of rbcS and rbcL in the leaves at different positions in transgenic rice plants with decreased content of Rubisco.2004

    • Author(s)
      M.Ishizuka, A.Makino*, Y.Suzuki, T.Mae
    • Journal Title

      Soil Sci.Plant Nutr. 50

      Pages: 233-239

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Changes in the thermal dissipation and the electron flow in the water-water cycle in rice grown under conditions of physiologically low temperature.2004

    • Author(s)
      N.Hirotsu, A.Makino*, A.Ushio, T.Mae
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 635-644

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 生物化学2005

    • Author(s)
      牧野 周を含む他14名
    • Total Pages
      279
    • Publisher
      朝倉書店
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Photosynthesis, In Biological Chemistry(Edited by Onodera et al.)2005

    • Author(s)
      A.Makino et al.
    • Total Pages
      279
    • Publisher
      Asakura-syoten
    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2007-12-13  

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