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2004 Fiscal Year Final Research Report Summary

Study of osmoregulation in birds

Research Project

Project/Area Number 14360165
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionNagoya University

Principal Investigator

SAITO Noboru  Nagoya University, Graduate School of Bioagricutural Sciences, Associate Professor, 大学院・生命農学研究科, 助教授 (40211924)

Co-Investigator(Kenkyū-buntansha) SHIMADA Kiyoshi  Nagoya University, Graduate School of Bioagricutural Sciences, Professor, 大学院・生命農学研究科, 教授 (40065579)
TSUKADA Akira  Nagoya University, Graduate School of Bioagricutural Sciences, Assistant Professor, 大学院・生命農学研究科, 助手 (20343212)
Project Period (FY) 2002 – 2004
Keywordsosmotic stimulation / chicken / aquaporin / vasotocin / gene expression
Research Abstract

For long time, it has been not clear about the osmoreceptor on the osmoregulation. Recently, aquaporin 4(AQP4) has been a candidate as an osmoreceptor, but there are no clear evidence about that. We cloned chicken AQP4 cDNA and determined its sequence. We analyzed the gene expression of AQP4 in the chicken hypothalamus in related with osmostimulation. AQP4 gene expression in the chicken increased by hyperosmolality. Threrefore, these results suggest that AQP4 may involve osmoreceptor in the chicken. Since there are no observations about the increase of AQP4 gene expression by hyperosmolality in the mammals, such increase is specific thing in the chicken.
Comparing arginine vasotocin(AVT) gene expression between layer and broiler strain after water deprivation, both strains chick decreased body weights and increased plasma osmolality. On AVT gene expression, AVT gene expression increased in layer but not in broiler. These results suggested that there was some different mechanism of osmoregulation between two strains.
It has been considered that neuropeptide Y(NPY) is a important role for feeding behavior. But, after water deprivation, NPY gene expression increased in the chicken. Therefore, these data suggest that NPY has a pivotal role on the water homeostasis in the chicken.

  • Research Products

    (23 results)

All 2005 2004 2003 2002

All Journal Article (23 results)

  • [Journal Article] Identification of spermatogenic cells expressiong protamine mRNA in Japanese quail by RT-PCR,2005

    • Author(s)
      Upi Chairun Nisa, Tsukada A., Takagi S., Yamamoto I., Ha Y., Hrabia A., Saito N.
    • Journal Title

      J.Poult.Sci. 42

      Pages: 70-78

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cloning of duck PRL cDNA and genomic DNA2005

    • Author(s)
      Kansaku N, Ohkubo T, Okabayashi H, Guemene D, Kuhnlein U, Zadworny D, Shimada K.
    • Journal Title

      General and Comparative Endocrinology. 141

      Pages: 39-47

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification of Spermatogenic cells expressiong protamine mRNA in Japanese quail by RT-PCR2005

    • Author(s)
      Upi Chairun Nisa, Tsukada A., Takagi S., Yamamoto I., Ha Y., Hrabia A., Saito N., Shimada K.
    • Journal Title

      J.Poult.Sci. 42

      Pages: 70-78

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cloning of duck PRL CDNA and genomic DNA.2005

    • Author(s)
      Kansaku N, Ohkubo T, Okabayashi H, Guemene D, Kuhniein U, Zadworny D, Shimada K.
    • Journal Title

      Gen Comp Endocrinol. 141

      Pages: 39-47

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Increased expression of elastolytic cysteine proteases, cathepsins S and K, in the neointima of balloon-Injured at carotird arteries2004

    • Author(s)
      Cheng XW, Kuzuya M, Sasaki T, Arakawa K, Kanda S, Sumi D, Koike T, Maeda K, Tamaya-Mori N, Shi GP, Saito N, Iguchi A.
    • Journal Title

      Am.J.Pathology 164

      Pages: 243-251

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Changes in mRNA expression of MMP-2 in the Mullerian duct of chicken embryo.2004

    • Author(s)
      Ha Y., Tsukada A., Saito N., Shimada K.
    • Journal Title

      General and Comparative Endocrinology. 139

      Pages: 131-136

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Photoinducible phase-specific light induction of Cry1 gene in the pars tuberalis of Japanese quail.2004

    • Author(s)
      Yasuo S, Watanabe M, Tsukada A, Takagi T, Iigo M, Shimada K, Ebihara S, Yoshimura T.
    • Journal Title

      Endocrinology 145

      Pages: 1612-1616

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effect of central administration of prolactin-releasing peptide on feeding in chicks2004

    • Author(s)
      Tachibana T, Saito S, Tomonaga S, Takagi T, Saito ES, Nakanishi T, Koutoku T, Tsukada A, Ohkubo T, Boswell T, Furuse M
    • Journal Title

      Physiol Behav 80

      Pages: 713-719

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effect of central administration of prolactin-releasing peptide on feeding in chicks.2004

    • Author(s)
      Tachibana T, Saito S, Tomonaga S, Takagi T, Saito ES, Nakanishi T, Koutoku T, Tsukada A, Ohkubo T, Boswell T, Furuse M.
    • Journal Title

      Physiol Behav 80

      Pages: 713-719

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Changes in mRNA expression of MMP-2 in the Mullerian duct of chicken embryo2004

    • Author(s)
      Ha Y., Tsukada A., Saito N., Shimada K.
    • Journal Title

      Gen and Comp Endocrinol 139

      Pages: 131-136

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effect of endocrine disrupters on mRNA expression of vitellogenin(VTG) II and very low density lipoprotein(apoVLDL) II in the liver of quail embryos.2003

    • Author(s)
      Ichikawa K., Ha Y., Tsukada A., Saito N., Shimada K.
    • Journal Title

      J.Poult.Sci. 40

      Pages: 45-52

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Profiling of mRNA expression of genes related to sex differentiation of the gonads in the chicken embryo2003

    • Author(s)
      Yamamoto I., Tsukada A., Saito N., Shimada K.
    • Journal Title

      Poult.Sci. 82

      Pages: 1462-1467

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of protgamine mRNA in relation to spermatogenic activity in Japanese quail.2003

    • Author(s)
      Upi Chairun Nisa, Tsuakda A., Mori M., Saito N., Shimada K.
    • Journal Title

      J.Poult.Sci. 40

      Pages: 309-318

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular characterization of chicken growth hormone secretagogue receptor gene.2003

    • Author(s)
      Tanaka M, Miyazaki T, Yamamoto I, Nakai N, Ohta Y, Tsushima N, Wakita M, Shimada K.
    • Journal Title

      Gen Comp Endocrinol. 134

      Pages: 198-202

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] cDNA cloning of chicken orexin receptor and tissue distribution : sexually dimorphic expression in chicken gonads.2003

    • Author(s)
      Ohkubo T, Tsukada A, Shamoto K.
    • Journal Title

      J Mol Endocrinol. 31

      Pages: 499-508

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] cDNA cloning and mRNA expression of estrogen receptor a in Japanese quail.2003

    • Author(s)
      Ichikawa K., Yamamoto I., Tsukada A., Saito N., Shimada K.
    • Journal Title

      J.Poult.Sci. 40

      Pages: 121-129

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effect of endocrine disrupters on mRNA expression of vitellogenin(VTG) II and very low density lipoprotein (apoVLDL)II in the liver of quail embryos.2003

    • Author(s)
      Ichikawa K., Ha Y., Tsukada A., Saito N., Shimada K.
    • Journal Title

      J.Poult.Sci. 40

      Pages: 45-52

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Profiling of mRNA expression of genes related to sex differentiation of the gonads in the chicken embryo.2003

    • Author(s)
      Yamamoto I., Tsukada A., Saito N., Shimada K.
    • Journal Title

      Poultry Sci. 82

      Pages: 1462-1467

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Molecular characterization of chicken growth hormone secretagogue receptor gene.2003

    • Author(s)
      Tanaka M, Miyazaki T, Yamamoto I, Nakai N, Ohta Y, Tsushima N, Wakita M, Shimada K.
    • Journal Title

      Gen Comp Endocrinol 134

      Pages: 198-202

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Supression effect of p-nonylphenol on male-specific mRNA expression in the embryonic gonad of chickens.2002

    • Author(s)
      Sakimura M., Usami M., Hanzawa S., Tsukada A., Saito N., Ohno Y., Shimada K.
    • Journal Title

      J.Poult.Sci. 39

      Pages: 91-99

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effect of estradiol and nonylpehnol on mRNA expression of estrogen receptors α and β, and cytocherome P450 aroamtase in the gonad of chicken embryos2002

    • Author(s)
      Sakimura M., Tsukada A., Usami M., Hanzawa S., Saito N., Ohno Y., Shimada K.
    • Journal Title

      J.Poult.Sci. 39

      Pages: 302-309

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] cDNA cloning and mRNA expression of transformer 2(Tra2) in chicken embryo.2002

    • Author(s)
      Yamamoto I., Tsukada A., Saito N., Shimada K.
    • Journal Title

      Biochem.Biophy.Acta 1579

      Pages: 185-188

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effect of estradiol and nonylpehnol on mRNA expression of estrogen receptors α and β, and cytochrome P450 aroamtase in the gonad of chicken embryos.2002

    • Author(s)
      Sakimura M., Tsukada A., Usami M., Hanzawa S., Saito N., Ohno Y., Shimada K.
    • Journal Title

      J.Poult.Sci. 39

      Pages: 302-309

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2006-07-11  

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