2005 Fiscal Year Final Research Report Summary
Development and applications of a protozoan parasite vector using Toxoplasma gondii to express heterologous genes
| Project/Area Number |
14360172
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
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Principal Investigator |
XUAN Xuenan Obihiro University, Professor, 原虫病研究センター, 教授 (10292096)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOYAMA Naoaki Obihiro University, Assoc.Prof., 原虫病研究センター, 助教授 (80301802)
INOUE Noboru Obihiro University, Assoc.Prof., 原虫病研究センター, 助教授 (10271751)
TANAKA Tetsuya Hokkaido University, Assist.Prof., 農学部, 助手 (00322842)
IWATA Akira Nippon Institute for Biological science, Senior Researcher, 主任研究員 (70193745)
TAKASHIMA Yasuhiro Gifu University, Assoc.Prof., 応用生物科学部, 助教授 (20333552)
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| Project Period (FY) |
2002 – 2005
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| Keywords | T.gondii / N.canunim / C.parvum / NcSRS2 / CpP23 / Recombinant vaccine / Vector vaccine / Protection |
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| Research Abstract |
An improved method for the construction of recombinant Toxoplasma gondii expressing heterologous genes was developed using pyrimethamine-resistant DHFR-TS and green fluorescent protein (GFP) genes as double selection markers. This improved method allows the construction of recombinant T.gondii expressing foreign genes with great case and speed. Because the foreign genes were placed between the DHFR-TS and GFP genes, all of the drug-resistant and fluorescent clones were confirmed to be expressed foreign genes. This method was employed to construct recombinant T.gondii expressing the Neospora caninum major antigen SRS2 gene (Tg/NcSRS2) and the Cryptosporidium parvum major antigen P23 gene (Tg/CpP23). The expression levels of NcSRS2 and CpP23 in recombinant Tg/NcSRS2 and Tg/CpP23 were similar to those in N.caninum and C.parvum, respectively. The molecular weight and antigenic property of recombinant NcSRS2 and CpP23 were similar to those of native NaSRS2 and CpP23, respectively. Mice immunized with recombinant Tg/NcSRS2 were partially protected from N.caninum challenge infection. On the other hand, the mice immunized with recombinant Tg/CpP23 produced specific neutralizing antibodies against C.parvum. In addition, a bioactive molecule, such as mouse interferon gamma was successfully expressed by T.gondii vector. These results indicate that the T.gondii vector may provide a new tool not only for the production of recombinant vaccines against protozoan parasite infections in animals, but also for the production of bioactive molecules.
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Research Products
(13 results)
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[Journal Article] Identification and characterization of cross-reactive antigens from Neospora caninum and Toxoplasma gondii.2005
Author(s)
Liao, M., Xuan, X., Shirafuji, H., Fukumoto, S., Hirata, H., Suzuki, H., Fujisaki, K.
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Journal Title
Parasitol. 130
Pages: 481-488
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Expression of P23 of Cryptosporidium parvum in Toxoplasma gondii and evaluation of its protective effects.2005
Author(s)
Shirafuji, H., Xuan, X., Kimata, I., Takashima, Y., Shinya, F., Otsuka, H., Nagsdawa, H., Suzuki, H.
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Journal Title
J.Parasitol. 91
Pages: 476-479
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Natural IgM antibodies in sera from various animals but not the cat kill Toxoplasma gondii by activating complement classical pathway.2004
Author(s)
Kaneko, Y., Takashima, Y., Xuan, X., Igarashi, I., Nagasawa, H., Mikami, T., Otsuka, H.
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Journal Title
Parasitol. 128
Pages: 123-129
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characterization of Toxoplasma gondii engineered to express mouse interferon-gamma.2003
Author(s)
Nishikawa, Y., Xuan, X., Makala, L., Vielemeyer, O., Joiner, K.A., Nagasawa, H.
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Journal Title
Int.J.Parasitol 33
Pages: 1525-1535
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Recombinant feline herpesvirus type 1 expressing Toxoplasma gondii ROP2 antigen inducible protective immunity in cats.2002
Author(s)
Mishima, M., Xuan, X., Yokoyama, N., Igarashi, I., Fujisaki, K., Nagasawa, H., Mikami, T.
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Journal Title
Parasitol.Res. 88
Pages: 144-149
Description
「研究成果報告書概要(欧文)」より
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[Book] 獣医微生物学2003
Author(s)
玄学南(見上彪監修)
Total Pages
307-312
Publisher
文永堂出版
Description
「研究成果報告書概要(和文)」より
