2004 Fiscal Year Final Research Report Summary
THE ANALYSIS ON MOLECULAR MECHANISMS OF PLANT RESISTANCE BREAKING BY POLYXENY-PATHOGENIC FUNGI
| Project/Area Number |
14360195
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物資源科学
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| Research Institution | IBARAKI UNIVERSITY |
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Principal Investigator |
AKUTSU Katsumi IBARAKI UNIVERSITY, COLLEGE OF AGRICULTURE, PROFESSOR, 農学部, 教授 (10151002)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Masami IBARAKI UNIVERSITY, COLLEGE OF AGRICULTURE, ASSISTANT PROFESSOR, 農学部, 助教授 (70301075)
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| Project Period (FY) |
2002 – 2004
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| Keywords | Botrytis cinerea / G protein genes / signal transduction system / transformation / pathogenisity gene family / resistance breaking / Botrytis spp. / Agrobacterium method |
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| Research Abstract |
Gα subunit genes, bcg1 and bcg2, of the gray mold fungus Botrytis cinerea have been previously cloned and characterized. We have isolated a novel Gα subunit gene (bcg3) and a Gβ subunit gene (bcgb1), only one copy each was present in the genome. The deduced amino acid sequences of both Bcg3 and Bcgb1 show the typical conserved motifs present in Gα or Gβ proteins from other filamentous fungi. Bcg3 is a member of the Gas class (III), which also includes MagA of Magnaporthe grisea and Gpa3 of Ustilag maydis. Bcgb1 displays a high degree of sequence homology (approximately 90%) to Gβ subunits Fgb1, Cpgb1, and Mgb1 from Fusarium oxysporum, Cryphonectria parasitica, and M.grisea, respectively. Reverse-transcription (RT)-PCR experiments show clearly that both genes are expressed at very early stages of the infection process. Analysis of transcript levels indicated that bcg3 expression levels are extremely low, whereas bcgb1 expression levels are similar to bcg1 during the infection process. Next, the isolation of the gene, which peculiarly appeared in the differentiation of the multicellular appressoria, was tried by Differential Display method. The result showed that there was a difference between both samples in the band pattern of PCR amplified product with the polyacrylamide electrophoresis, when sevaral primer was used. The base sequences of the bands peculiarly detected in the appressorium differentiation were decided. The amino acid sequence of which the one band was anticipated showed the high homology for the arrangement of aspartic proteinase. Aspartic proteinase is known as an enzyme peculiarly secreted in the infection in opportunistic infection fungi such as Aspergillus fumigates and Candida albicans, and it is suggested to be deeply concerned in the pathogenicity. At present, the roles in the formation of the appressorium and the infection are analyzed by disruption of the aspartic proteinase gene.
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Research Products
(26 results)
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[Journal Article] Transgenic cucumber expressing an endogenous Class III chitinase gene (CHI2) shows reduced symptoms of Botrytis cinerea2004
Author(s)
Kishimoto, K., Nishizawa, Y., Tabei, Y., Nakajima, M., Hibi, T., Akutsu, K.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Epidemiological Research on Botrytis Diseases of Tulip Plants Caused by B.tulipae and B.cinerea2004
Author(s)
Yamada, K., Aoki, T., Ikeda, C., Ichiman, Y., Kanno, Y., Suzuki, T., Nagashima, H., Nakajima, M., Akutsu, K.
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Journal Title
Acta Horticulturae 451
Pages: 157-161
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cloning of various promoters for foreigen gene expression in Erwinia ananasres2004
Author(s)
Numata, S., Ui, S., Tomiyama, M., Hasebe, A, Nakajima, M., Akutsu, K.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Response of transgenic cucumber expressing a rice class I chitinase gene to two fungal pathogens with different infectivities2003
Author(s)
Kishimoto, K, Nakajima, M., Nishizawa, Y., Tabei, Y., Hibi, T., Akutsu K.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Biological control of rice blast by the epiphytic bacterium Erwinia ananas transformed with a chitinolytic enzyme gene from an antagonistic bacterium, Serratia marcescens strain B22003
Author(s)
Someya, N., Numata, M., Nakajima, M., A.Hasebe, Hibi, T., Akutsu, K
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Induced Resistance to Rice Blast by Antagonistic Bacterium, Serratia marcescens Strain B22002
Author(s)
Someya, N., Nakajima, M., Hibi, T., Yamaguchi, I., Akutsu, K.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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