2004 Fiscal Year Final Research Report Summary
Production and analyses of mice deficient in molecules involved in cell polarization
| Project/Area Number |
14370003
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Gunma University |
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Principal Investigator |
HARADA Akihiro Gunma Univ., Inst.Mol.Cell.Regul., Professor, 生体調節研究所, 教授 (40251441)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Kensuke Gunma Univ., Inst.Mol.Cell.Regul., Associate Professor, 生体調節研究所, 助教授 (50218567)
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| Project Period (FY) |
2002 – 2004
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| Keywords | polarized transport / cell polarity / epithelial cell / neuron / Cre-loxP / gene targeting / rab proteins / SNARE proteins |
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| Research Abstract |
Cell polarity is crutial for function of various types of cells. For cell polarity, polarized transport within cells are thought to be essential. A number of proteins (e.g, rab proteins and SNARE proteins) have been shown to be involved in polarized transport. However, their roles in the development and function of tissues and whole body of animals are largely unknown. Therefore, to uncover their roles in the development and function in the animals, we are making knockout mice for each of these proteins. As it is possible that these molecules are involved in embryonic development, simply knocking out of these genes may lead to embryonic lethality at very early stages of development. To circumvent this problem, we are making conditional knockout mice using Cre-loxP system.
During the term of grant, we have completed making conditional knockout mice of these proteins and are now analyzing their phenotypes. In addition, we analyzed AP3B knockout mice under electron microscopy and showed that the diameter of their synaptic vesicles became enlarged, which may lead to the neurological phenotype (convulsion) of this knockout mice. We also looked at the polarization process of neurons in vitro and observed that (1)axons were able to be formed from dendrites (2)axonogenesis is delayed in inhibitory neurons compared to excitatory neurons.
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[Journal Article] Defective function of GABA-containing synaptic vesicles in mice lacking the AP-3B clathrin adaptor.2004
Author(s)
Nakatsu F, Okada M, Mori F, Kumazawa N, Iwasa H, Zhu G, Kasagi Y, Kamiya H, Harada A, Nishimura K, Takeuchi A, Miyazaki T, Watanabe M, Yuasa S, Manabe T, Wakabayashi K, Kaneko S, Saito T, Ohno H.
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Journal Title
J.Cell Biol. 167(2)
Pages: 293-302
Description
「研究成果報告書概要(欧文)」より
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