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2004 Fiscal Year Final Research Report Summary

Study of DNA methylation and gene expression during development of male reproductive organs. -Global analysis of DNA methylation using Restriction Landmark Genomic Scanning (RLGS) method-

Research Project

Project/Area Number 14370004
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionChiba University

Principal Investigator

MORI Chisato  Chiba university, graduate school of medicine, professor, 大学院・医学研究院, 教授 (90174375)

Co-Investigator(Kenkyū-buntansha) FUKATA Hideki  Chiba university, graduate school of medicine, associate professor, 大学院・医学研究院, 助教授 (00359598)
KOMIYAMA Masatoshi  Chiba university, center for environment, health and field sciences, lecturer, 講師 (70175339)
KODA Masao  Chiba university, graduate school of medicine, assistant professor, 大学院・医学研究院, 助手 (50361449)
TODAKA Emiko  Chiba university, center for environment, health and field sciences, assistant professor, 助手 (30334212)
Project Period (FY) 2002 – 2004
Keywordsreproductive organ / development / DNA methylation / RLGS method / gene expression / endocrine disruptor / diethylstilbestrol / epigenetics
Research Abstract

Neonatal C57BL/6 mice were exposed to 3 μg/pup/day of synthetic estrogen, diethylstilbestrol(DBS). At day 30, epididymidis (from male mice) and uteri (from female mice) were teken, and their status of DNA methylation were analyzed using restriction landmark genomic scanning method (RLGS). In epididymis and uterus, the methylation of 7 and 11 loci (out of about 1000 loci) were changed, respectively. Then lower dose of DBS (0.3、0.03、0.003 μg/pup/day) were examined, and clear dose dependency of the methylation change was observed.
We analyzed the loci those methylation were changed by DBS. Four loci from epididymis and 3 loci from uterus were cloned, and the characteristics of them such as GC content, chromosomal distribution, neighbor genes were examined. Four loci from epididymis were near from zinc finger protein, RNA helicase, protein kinase associated gene, retinal disease associated gene, respectively. Three loci from uterus were not close to known genes.
The mechanism by which methylation was changed, and the effect of that change on development of male reproductive organs remained to be elucidated.

  • Research Products

    (26 results)

All 2005 2004 2003 2002

All Journal Article (24 results) Book (2 results)

  • [Journal Article] Necessity to measure PCBs and organochlorine pesticide concentrations in human umbilical cords for fetal exposure assessment.2005

    • Author(s)
      Hideki Fukata
    • Journal Title

      Environmental Health Perspectives 113

      Pages: 297-303

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Epigenetic alteration by the chemical substances, food and environmental factors.2004

    • Author(s)
      Fukata, Hideki
    • Journal Title

      Reproductive Medicine and Biology 3

      Pages: 115-121

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Long-term alteration of gene expression without morphological change in testis after neonatal exposure to genistein in mice : toxicogenomic analysis using cDNA microarray.2004

    • Author(s)
      Adachi, Tetsuya
    • Journal Title

      Food and Chemical Toxicology 42

      Pages: 445-452

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Toxicogenomic difference between diethylstilbestrol and 17β-estradiol in mouse testicular gene expression by neonatal exposure.2004

    • Author(s)
      Adachi, Tetsuya
    • Journal Title

      Molecular Reproductive Development 67

      Pages: 19-25

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Gene expression analysis of the rat testis after treatment with di(2-ethlhexyl) phthalate using cDNA microarray and real-time RT-PCR.2004

    • Author(s)
      Kijima, Kazuyasu
    • Journal Title

      Toxicology and Applied Pharmacology 200

      Pages: 103-110

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effect of neonatal exposure to diethylstilbestrol on testicular gene expression in adult mouse : comprehensive analysis with cDNA subt-raction method.2004

    • Author(s)
      Matsuno, Yoshiharu
    • Journal Title

      International Journal of Andrology 27

      Pages: 115-122

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] High-risk group and high-risk life stage : Key issues in adverse effects of environmental agents on human health.2004

    • Author(s)
      Mori, Chisato
    • Journal Title

      Reproductive Medicine and Biology 3

      Pages: 51-58

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Percoll fractionation of adult mouse spermatogonia improves germ cell transplantation.2004

    • Author(s)
      Koh, Kyu-Bom
    • Journal Title

      Asian Journal of Andrology 6

      Pages: 93-98

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Flutamide induces ultrastructural changes in spermatids and the ectoplasmic specialization between the Sertoli cell and spermatids in mouse testes.2004

    • Author(s)
      Anahara, Reiko
    • Journal Title

      Reproductive Toxicology 18

      Pages: 589-596

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 胎児を基準とした化学物質のリスク評価と対策の開発の試み2004

    • Author(s)
      深田秀樹
    • Journal Title

      ファルマシア 40

      Pages: 328-332

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Epigenetic alteration by the chemical substances, food and environ-mental factors.2004

    • Author(s)
      Hideki Fukata
    • Journal Title

      Reproductive Medicine and Biology 3

      Pages: 115-121

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Long-term alteration of gene expression without morphological change in testis after neonatal exposure to genistein in mice : toxicogenomic analysis using cDNA microarray.2004

    • Author(s)
      Tetsuya Adachi
    • Journal Title

      Food and Chemical Toxicology 42

      Pages: 445-452

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Toxicogenomic difference between diethylstilbestrol and 17β-estradiol in mouse testicular gene expression by neonatal exposure.2004

    • Author(s)
      Tetsuya Adachi
    • Journal Title

      Molecular Reproductive Development 67

      Pages: 19-25

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Gene expression analysis of the rat testis after treatment with di(2-ethylhexyl) phthalate using cDNA microarray and real-time RT-PCR.2004

    • Author(s)
      Kazuyasu Kijima
    • Journal Title

      Toxicology and Applied Pharmacology 200

      Pages: 103-110

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effect of neonatal exposure to diethylstilbestrol on testicular gene expression in adult mouse : comprehensive analysis with cDNA subt-raction method.2004

    • Author(s)
      Yoshiharu Matsuno
    • Journal Title

      International Journal of Andrology 27

      Pages: 115-122

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] High-risk group and high-risk life stage : Key issues in adverse effects of environmental agents on human health.2004

    • Author(s)
      Chisato Mori
    • Journal Title

      Reproductive Medicine and Biology 3

      Pages: 51-58

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Percoll fractionation of adult mouse spermatogonia improves germ cell transplantation.2004

    • Author(s)
      Kyu-Bom Koh
    • Journal Title

      Asian Journal of Andrology 6

      Pages: 93-98

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Flutamide induces ultrastructural changes in spermatids and the ectoplasmic specialization between the Sertoli cell and spermatids in mouse testes.2004

    • Author(s)
      Reiko Anahara
    • Journal Title

      Reproductive Toxicology 18

      Pages: 589-596

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Association of testicular undescent induced by prenatal flutamide treatment with thickening of the cremaster muscle in rats.2003

    • Author(s)
      Matsuno, Yoshiharu
    • Journal Title

      Reproductive Medical Biology 2

      Pages: 109-113

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Detection of mitomycin C-induced testicular toxicity by micronucleus assay in mice.2003

    • Author(s)
      Nakagawa, Soichi
    • Journal Title

      Reproductive Medicine and Biology 2

      Pages: 69-72

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Association of testicular undescent induced by prenatal flutamide treatment with thickening of the cremaster muscle in rats.2003

    • Author(s)
      Yoshiharu Matsuno
    • Journal Title

      Reproductive Medical Biology 2

      Pages: 109-113

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Detection of mitomycin C-induced testicular toxicity by micronucleus assay in mice.2003

    • Author(s)
      Soichi Nakagawa
    • Journal Title

      Reproductive Medicine and Biology 2

      Pages: 69-72

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Temporal changes in testis-weight during the last 50 years in Japan.2002

    • Author(s)
      Mori, Chisato
    • Journal Title

      Anatomical Science International 77

      Pages: 109-116

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Temporal changes in testis-weight during the last 50 years in Japan.2002

    • Author(s)
      Chisato Mori
    • Journal Title

      Anatomical Science International 77

      Pages: 109-116

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 新編精子学2005

    • Author(s)
      森 千里
    • Publisher
      東京大学出版会
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 未来世代のために -新米医学部教授の七転八倒-2004

    • Author(s)
      森 千里
    • Total Pages
      215
    • Publisher
      環境新聞社
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2006-07-11  

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